Enhanced activity toward PET by site-directed mutagenesis of Thermobifida fusca cutinase-CBM fusion protein

Carbohydrate Polymers

Volumn 97, Issue 1, 2013, Pages 124-129

  Zhang, Yao ^a,b   Wang, Lei ^a   Chen, Jian ^a   Wu, Jing ^a  

^a JIANGNAN UNIVERSITY   (China)

^b SHANDONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

Cutinase CBM; Polyethylene terephthalate; Site directed mutagenesis; Thermobifida fusca

Indexed keywords

AMINO ACIDS; BINDING SITES; BINS; ENZYMES; ESCHERICHIA COLI; HYDROGEN BONDS; HYDROPHOBICITY; MUTAGENESIS; POLYETHYLENE TEREPHTHALATES; PROTEINS;

CARBOHYDRATE-BINDING MODULES; CATALYTIC EFFICIENCIES; CUTINASE-CBM; ENZYME CHARACTERIZATION; HYDROPHOBIC INTERACTIONS; POLYETHYLENE TEREPHTHALATE FIBERS; SITE DIRECTED MUTAGENESIS; THERMOBIFIDA FUSCA;

PLASTIC BOTTLES;

ESCHERICHIA COLI; THERMOBIFIDA FUSCA;

CARBOXYLESTERASE; CUTINASE; HYBRID PROTEIN; POLYETHYLENE TEREPHTHALATE;

ACTINOMYCETALES; BINDING SITE; BIOCATALYSIS; BIOSYNTHESIS; CHEMICAL PHENOMENA; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; HYDROGEN BOND; HYDROLYSIS; METABOLISM; PH; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS; TEMPERATURE;

ACTINOMYCETALES; BINDING SITES; BIOCATALYSIS; CARBOXYLIC ESTER HYDROLASES; ESCHERICHIA COLI; HYDROGEN BONDING; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; MUTAGENESIS, SITE-DIRECTED; POLYETHYLENE TEREPHTHALATES; PROTEIN STABILITY; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84878008702     PISSN: 01448617     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.carbpol.2013.04.042     Document Type: Article

References (20)

1. Alisch, M., Feuerhack, A., Muller, H., Mensak, B., Andreaus, J., & Zimmermann, W. (2004). Biocatalytic modification of polyethylene terephthalate fibres by esterases from aceinomycete isolates. Biocatalysis and Biotransformation, 22(5), 347-351. (Pubitemid 40425618)
2. Araujo, R., Casal, M., & Cavaco-Paulo, A. (2008). Application of enzymes for textile fibres processing. Biocatalysis and Biotransformation, 26(5), 332-349.
3. Araujo, R., Silva, C., O'Neill, A., Micaelo, N., Guebitz, G., Soares, C. M., et al. (2007). Tailoring cutinase activity towards polyethylene terephthalate and polyamide 6, 6 fibers. Journal of Biotechnology, 128(4), 849-857. (Pubitemid 46341488)
4. Arpigny, J. L., & Jaeger, K. E. (1999). Bacterial lipolytic enzymes: Classification and properties. Biochemical Journal, 343(Pt 1), 177-183. (Pubitemid 29473893)
5. Carla, S., & Cavaco-Paulo, A. (2008). Biotransformations in synthetic fibres. Biocatalysis and Biotransformation, 26(5), 350-356.
6. Carvalho, C. M., Aires-Barros, M. R., & Cabral, J. M. (1999). Cutinase: From molecular level to bioprocess development. Biotechnology and Bioprocess Engineering, 66(1), 17-34.
7. Chen, S., Tong, X., Woodard, R. W., Du, G., Wu, J., & Chen, J. (2008). Identification and characterization of bacterial cutinase. Journal of Biological Chemistry, 283(38), 25854-25862.
8. Degani, O., Gepstein, S., & Dosoretz, C. G. (2002). Potential use of cutinase in enzymatic scouring of cotton fiber cuticle. Applied Biochemistry and Biotechnology, 103(1-6), 277-289.
9. Din, N., Forsythe, I. J., Burtnick, L. D., Gilkes, N. R., Miller, R. C., Jr., Warren, R. A. J., et al. (1994). The cellulose-binding domain of endoglucanase A (CenA) from Cellulomonas fimi: Evidence for the involvement of tryptophan residues in binding. Molecular Microbiology, 11(4), 747-755. (Pubitemid 24197467)
10. Egmond, M. R., & de Vlieg, J. (2000). Fusarium solani pisi cutinase. Biochimie, 82(11), 1015-1021.
11. Guebitz, G. M., & Cavaco-Paulo, A. (2008). Enzymes go big: Surface hydrolysis and functionalization of synthetic polymers. Trends in Biotechnology, 26(1), 32-38. (Pubitemid 350298596)
12. Herrero Acero, E., Ribitsch, D., Steinkellner, G., Gruber, K., Eiteljoerg, I., Trotscha, E., et al. (2011). Enzymatic surface hydrolysis of PET: Effect of structural diversity on kinetic properties of cutinases from Thermobifida. Macromolecules, 44(12), 4632-4640.
13. Hiraishi, T., Hirahara, Y., Doi, Y., Maeda, M., & Taguchi, S. (2006). Effects of mutations in the substrate-binding domain of poly[(R)-3- hydroxybutyrate] (PHB) depolymerase from Ralstonia pickettii T1 on PHB degradation. Applied and Environmental Microbiology, 72(11), 7331-7338. (Pubitemid 44748450)
14. Irwin, D. C., Zhang, S., & Wilson, D. B. (2000). Cloning, expression and characterization of a family 48 exocellulase, Cel48A, from Thermobifida fusca. European Journal of Biochemistry, 267, 4988-4997. (Pubitemid 30641294)
15. Levy, I., & Shoseyov, O. (2002). Cellulose-binding domains: Biotechnological applications. Biotechnology Advances, 20(3-4), 191-213. (Pubitemid 35326477)
16. Quentin, M., Ebbelaar, M., Derksen, J., Mariani, C., & van Der Valk, H. (2002). Description of a cellulose-binding domain and a linker sequence from Aspergillus fungi. Applied Microbiology and Biotechnology, 58, 658-662. (Pubitemid 34299231)
17. Ronkvist, A. M., Xie, W., Lu, W., & Gross, R. A. (2009). Cutinasecatalyzed hydrolysis of poly(ethylene terephtalate). Macromolecules, 42, 5128-5138.
18. Silva, C., Da, S., Silva, N., Matama, T., Araujo, R., Martins, M., et al. (2011). Engineered Thermobifida fusca cutinase with increased activity on polyester substrates. Biotechnology Journal, 6(10), 1230-1239.
19. Yoon, M. Y., Kellis, J., & Poulose, A. J. (2002). Enzymatic modification of polyester. AATCC Review, 2(6), 33-36. (Pubitemid 34736113)
20. Zhang, Y., Chen, S., Xu, M., Cavoco-Paulo, A., Wu, J., & Chen, J. (2010). Characterization of Thermobifida fusca cutinase-carbohydrate-binding module fusion proteins and their potential application in bioscouring. Applied and Environmental Microbiology, 76(20), 6870-6876.