Automated cell-specific laser detection and ablation of neural circuits in neonatal brain tissue

Journal of Physiology

Volumn 591, Issue 10, 2013, Pages 2393-2401

  Wang, Xueying ^a   Hayes, John A ^a   Picardo, Maria Cristina D ^a   Del Negro, Christopher A ^a  

^a The College of William and Mary   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; AUTOMATION; BRAIN STEM; CELL FUNCTION; CELL SPECIFIC LASER DETECTION; CELL SPECIFICITY; CONFOCAL MICROSCOPY; CONTROLLED STUDY; IMAGING SOFTWARE; IN VITRO STUDY; LASER; MOUSE; NERVE CELL; NERVE CELL NETWORK; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PROCEDURES CONCERNING CELLS;

ALGORITHMS; ANILINE COMPOUNDS; ANIMALS; ANIMALS, NEWBORN; BRAIN STEM; CALCIUM; FEMALE; FLUORESCENT DYES; HOMEODOMAIN PROTEINS; LASER THERAPY; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; MICROSCOPY, CONFOCAL; NEURONS; PREGNANCY; SOFTWARE; XANTHENES;

EID: 84877810745     PISSN: 00223751     EISSN: 14697793     Source Type: Journal    
DOI: 10.1113/jphysiol.2012.247338     Document Type: Article

References (33)

1. Bargmann C & Avery L (1995). Laser killing of cells in Caenorhabditis elegans. In Caenorbabditis elegans: modern biological analysis of an organism, eds. Epstein, H & Shakes, D, pp. 225-250. Elsevier, Amsterdam .
2. Danneman PJ & Mandrell TD (1997). Evaluation of five agents/methods for anesthesia of neonatal rats. Lab Anim Sci 47, 386-395.
3. Eklof-Ljunggren E, Haupt S, Ausborn J, Dehnisch I, Uhlen P, Higashijima S & El Manira A (2012). Origin of excitation underlying locomotion in the spinal circuit of zebrafish. Proc Natl Acad Sci U S A 109, 5511-5516.
4. Feldman JL, Del Negro CA & Gray PA (2013). Understanding the rhythm of breathing: so near, yet so far. Annu Rev Physiol 75, 423-452.
5. Fox J, Barthold S, Davisson M, Newcomer C, Quimby F & Smith A (eds) (2007). Volume 3: the mouse in biomedical research, 2nd edition; normative biology, husbandry, and models . Academic Press, Burlington, MA .
6. Frermann D, Keller BU & Richter DW (1999). Calcium oscillations in rhythmically active respiratory neurones in the brainstem of the mouse. J Physiol 515, 119-131.
7. Goulding M (2009). Circuits controlling vertebrate locomotion: moving in a new direction. Nat Rev Neurosci 10, 507-518.
8. Gray PA, Hayes JA, Ling GY, Llona I, Tupal S, Picardo MC, Ross SE, Hirata T, Corbin JG, Eugenin J & Del Negro CA (2010). Developmental origin of preBötzinger complex respiratory neurons. J Neurosci 30, 14883-14895.
9. Grillner S (2006). Biological pattern generation: the cellular and computational logic of networks in motion. Neuron 52, 751-766.
10. Grillner S & Jessell TM (2009). Measured motion: searching for simplicity in spinal locomotor networks. Curr Opin Neurobiol 19, 572-586.
11. Hayes JA, Wang X & Del Negro CA (2012). Cumulative lesioning of respiratory interneurons disrupts and precludes motor rhythms in vitro. Proc Natl Acad Sci U S A 109, 8286-8291.
12. Hirata T, Li P, Lanuza GM, Cocas LA, Huntsman MM & Corbin JG (2009). Identification of distinct telencephalic progenitor pools for neuronal diversity in the amygdala. Nat Neurosci 12, 141-149.
13. Hochman S, Gozal EA, Hayes HB, Anderson JT, DeWeerth SP & Chang YH (2012). Enabling techniques for in vitro studies on mammalian spinal locomotor mechanisms. Front Biosci 17, 2158-2180.
14. Koizumi H, Wilson CG, Wong S, Yamanishi T, Koshiya N & Smith JC (2008). Functional imaging, spatial reconstruction, and biophysical analysis of a respiratory motor circuit isolated in vitro. J Neurosci 28, 2353-2365.
15. Koshiya N & Smith JC (1999). Neuronal pacemaker for breathing visualized in vitro. Nature 400, 360-363.
16. Lipton P (1999). Ischemic cell death in brain neurons. Physiol Rev 79, 1431-1568.
17. Liu KS & Fetcho Jr. (1999). Laser ablations reveal functional relationships of segmental hindbrain neurons in zebrafish. Neuron 23, 325-335.
18. Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES & Zeng H (2010). A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci 13, 133-140.
19. McKay LC, Janczewski WA & Feldman JL (2005). Sleep-disordered breathing after targeted ablation of preBötzinger complex neurons. Nat Neurosci 8, 1142-1144.
20. Meldrum BS (2002). Concept of activity-induced cell death in epilepsy: historical and contemporary perspectives. Prog Brain Res 135, 3-11.
21. Miller JP & Selverston A (1979). Rapid killing of single neurons by irradiation of intracellularly injected dye. Science 206, 702-704.
22. Okada Y, Sasaki T, Oku Y, Takahashi N, Seki M, Ujita S, Tanaka KF, Matsuki N & Ikegaya Y (2012). Preinspiratory calcium rise in putative pre-Bötzinger complex astrocytes. J Physiol 590, 4933-4944.
23. Paton JF (1996). The ventral medullary respiratory network of the mature mouse studied in a working heart-brainstem preparation. J Physiol 493, 819-831.
24. Pierani A, Moran-Rivard L, Sunshine MJ, Littman DR, Goulding M & Jessell TM (2001). Control of interneuron fate in the developing spinal cord by the progenitor homeodomain protein Dbx1. Neuron 29, 367-384.
25. Ruangkittisakul A, Okada Y, Oku Y, Koshiya N & Ballanyi K (2009). Fluorescence imaging of active respiratory networks. Respir Physiol Neurobiol 168, 26-38.
26. 2+ dependence of inspiratory-related rhythm in novel "calibrated" mouse brainstem slices. Respir Physiol Neurobiol 175, 37-48.
27. +] of confocally imaged respiratory center neurons in on-line-calibrated newborn rat brainstem slices. J Neurosci 26, 11870-11880.
28. + antagonism of inspiratory centre rhythms in slices and en bloc preparations of newborn rat brainstem. J Physiol 584, 489-508.
29. Schnell C, Fresemann J & Hulsmann S (2011). Determinants of functional coupling between astrocytes and respiratory neurons in the pre-Bötzinger complex. PLoS One 6, e26309.
30. Smith JC, Ellenberger HH, Ballanyi K, Richter DW & Feldman JL (1991). Pre-Bötzinger complex: a brainstem region that may generate respiratory rhythm in mammals. Science 254, 726-729.
31. Smith JC, Greer JJ, Liu GS & Feldman JL (1990). Neural mechanisms generating respiratory pattern in mammalian brain stem-spinal cord in vitro. I. Spatiotemporal patterns of motor and medullary neuron activity. J Neurophysiol 64, 1149-1169.
32. Svoboda K & Yasuda R (2006). Principles of two-photon excitation microscopy and its applications to neuroscience. Neuron 50, 823-839.
33. Volgin DV, Rukhadze I & Kubin L (2008). Hypoglossal premotor neurons of the intermediate medullary reticular region express cholinergic markers. J Appl Physiol 105, 1576-1584.