Molecular identification of nematode larvae different from those of the Trichinella genus detected by muscle digestion

Veterinary Parasitology

Volumn 194, Issue 2-4, 2013, Pages 117-120

  Marucci, Gianluca ^a   Interisano, Maria ^a   La Rosa, Giuseppe ^a   Pozio, Edoardo ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

12S mtDNA; 18S rDNA; COI; Digestion; ITS1; Metastrongylus; Molecular identification; Nematodes; Panagrolaimus; Toxocara; Trichinella

Indexed keywords

ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; BADGER; CROW; DOMESTIC PIG; FALSE POSITIVE RESULT; FILARIOIDEA; GENUS; HEN; LARVA; MEAT; METASTRONGYLUS; MOLECULAR GENETICS; MUSCLE DIGESTION; NEMATODE; NONHUMAN; PANAGROLAIMUS; PARASITE IDENTIFICATION; PARASITE ISOLATION; TOXOCARA CANIS; TOXOCARA CATI; TRICHINELLA; WILD BOAR; ANIMAL; BIRD; BIRD DISEASES; CHEMISTRY; CLASSIFICATION; DIFFERENTIAL DIAGNOSIS; DNA SEQUENCE; GENETICS; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; MUSCLE; MUSTELIDAE; PARASITOLOGY; POLYMERASE CHAIN REACTION; SWINE; SWINE DISEASES; TRICHINELLOSIS; VETERINARY;

CORVIDAE; FALCO TINNUNCULUS; FILARIOIDEA; METASTRONGYLUS; NEMATODA; PANAGROLAIMUS; SUS SCROFA; SUS SCROFA DOMESTICA; TOXOCARA; TOXOCARA CANIS; TOXOCARA CATI; TRICHINELLA; VERTEBRATA;

CYTOCHROME C OXIDASE; HELMINTH DNA; MITOCHONDRIAL DNA; RIBOSOMAL SPACER DNA; RIBOSOME DNA; RIBOSOME RNA; RNA 18S; RNA, RIBOSOMAL, 12S;

ANIMALS; BIRD DISEASES; BIRDS; DIAGNOSIS, DIFFERENTIAL; DNA, HELMINTH; DNA, MITOCHONDRIAL; DNA, RIBOSOMAL; DNA, RIBOSOMAL SPACER; ELECTRON TRANSPORT COMPLEX IV; FALSE POSITIVE REACTIONS; LARVA; MUSCLES; MUSTELIDAE; NEMATODA; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL; RNA, RIBOSOMAL, 18S; SEQUENCE ANALYSIS, DNA; SUS SCROFA; SWINE; SWINE DISEASES; TRICHINELLA; TRICHINELLOSIS;

EID: 84877699220     PISSN: 03044017     EISSN: 18732550     Source Type: Journal    
DOI: 10.1016/j.vetpar.2013.01.034     Document Type: Article

References (10)

1. Blaxter M.L. The promise of a DNA taxonomy. Philos. Trans. R. Soc. Lond. B: Biol. Sci. 2004, 359:669-679.
2. Blaxter M.L., De Ley P., Garey J.R., Liu L.X., Scheldeman P., Vierstraete A., Vanfleteren J.R., Mackey L.Y., Dorris M., Frisse L.M., Vida J.T., Thomas W.K. A molecular evolutionary framework for the phylum Nematoda. Nature 1998, 392:71-75.
3. Bowles J., McManus D.P. Genetic characterization of the Asian Taenia, a newly described taeniid cestode of humans. Am. J. Trop. Med. Hyg. 1994, 50:33-44.
4. Casiraghi M., Bain O., Guerrero R., Martin C., Pocacqua V., Gardner S.L., Franceschi A., Bandi C. Mapping the presence of Wolbachia pipientis on the phylogeny of filarial nematodes: evidence for symbiont loss during evolution. Int. J. Parasitol. 2004, 34:191-203.
5. De Ley P., De Ley I.T., Morris K., Abebe E., Mundo-Ocampo M., Yoder M., Heras J., Waumann D., Rocha-Olivares A., Jay Burr A.H., Baldwin J.G., Thomas W.K. An integrated approach to fast and informative morphological vouchering of nematodes for applications in molecular barcoding. Philos. Trans. R. Soc. Lond. B: Biol. Sci. 2005, 360:1945-1958.
6. Ferri E., Barbuto M., Bain O., Galimberti A., Uni S., Guerrero R., Ferté H., Bandi C., Martin C., Casiraghi M. Integrated taxonomy: traditional approach and DNA barcoding for the identification of filarioid worms and related parasites (Nematoda). Front. Zool. 2009, 7:1.
7. Hebert P.D., Cywinska A., Ball S.L., deWaard J.R. Biological identifications through DNA barcodes. Proc. Biol. Sci. 2003, 270:313-321.
8. Kang S., Tahera S., Loktev V.B., Wongratanacheewin S., Sohn W.M., Eom K.S., Park J.K. Molecular identification and phylogenetic analysis of nuclear rDNA sequences among three opisthorchid liver fluke species (Opisthorchiidae: Trematoda). Parasitol. Int. 2008, 57:191-197.
9. Lewis S.C., Dyal L.A., Hilburn C.F., Weitz S., Liau W.S., Lamunyon C.W., Denver D.R. Molecular evolution in Panagrolaimus nematodes: origins of parthenogenesis, hermaphroditism and the Antarctic species P. davidi. BMC Evol. Biol. 2009, 16:9-15.
10. Pozio E., Hoberg E., La Rosa G., Zarlenga D.S. Molecular taxonomy, phylogeny and biogeography of nematodes belonging to the Trichinella genus. Infect. Genet. Evol. 2009, 9:606-616.