Reexamining Michaelis-Menten enzyme kinetics for xanthine oxidase

American Journal of Physiology - Advances in Physiology Education

Volumn 37, Issue 1, 2013, Pages 37-48

  Bassingthwaighte, James B ^a   Chinn, Tamara M ^a  

^a University of Washington   (United States)

Author keywords

Bloodtissue exchange processes; Briggs haldane; Constrained parameter estimation; EC 1.17.3.2; Endothelial purine metabolism; Enzyme kinetics; JSim simulation analysis system; Michaelis Menten; Models as hypotheses; Product inhibition; Reaction reversibility; Reproducible research; Simultaneous optimization of multiple data sets; Xanthine oxidoreductase

Indexed keywords

EID: 84877659220     PISSN: 10434046     EISSN: 15221229     Source Type: Journal    
DOI: 10.1152/advan.00107.2012     Document Type: Article

References (20)

1. American Association for the Advancement of Science. Vision and Change: a Call to Action (online). http://visionandchange.org/files/2011/03/Revised-Vision-and-Change-Final-Report.pdf [29 November 2012].
2. American Association for the Advancement of Science and Howard Hughes Medical Institute. Scientific Foundations for Future Physicians (online). http://www.hhmi.org/grants/pdf/08-209_AAMC-HHMI_report. pdf [29 November 2012].
3. Bassingthwaighte JB, Chinard FP, Crone C, Goresky CA, Lassen NA, Reneman RS, Zierler KL. Terminology for mass transport and exchange. Am J Physiol Heart Circ Physiol 250: H539-H545, 1986.
4. Bassingthwaighte JB, Liebovitch L, West BJ. Fractal Physiology. Oxford: Oxford Univ. Press, 1994, p. 364.
5. Cao H, Pauff JM, Hille R. Substrate orientation and catalytic specificity in the action of xanthine oxidase. The sequential hydroxylation of hypoxanthine to uric acid. J Biol Chem 285: 28044-28053, 2010.
6. Chan IS, Goldstein AA, Bassingthwaighte JB. SENSOP: a derivativefree solver for non-linear least squares with sensitivity scaling. Ann Biomed Eng 21: 621-631, 1993.
7. Chizeck HJ, Butterworth E, Bassingthwaighte JB. Error detection and unit conversion. Automated unit balancing in modeling interface systems. IEEE Eng Med Biol Mag 28: 50-58, 2009.
8. Clement J. Model based learning as a key research area for science education. Int J Sci Educ 22: 1041-1053, 2000.
9. English BP, Min W, van Oijen AM, Lee KT, Luo G, Sun H, Cherayil BJ, Kou SC, Xie XS. Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited. Nat Chem Biol 2: 87-94, 2006.
10. Escribano J, Garcia-Canovas F, Garcia-Carmona F. A kinetic study of hypoxanthine oxidation by milk xanthine oxidase. Biochem J 254: 829-833, 1988.
11. Gardner TJ, Stewart JR, Casale AS, Downey JM. Reduction of myocardial ischemic injury with oxygen-derived free radical scavengers. Surgery 94: 423-427, 1983.
12. Hofmeyr JH, Cornish-Bowden A. The reversible Hill equation: how to incorporate cooperative enzymes into metabolic models. Comput Appl Biosci 13: 377-385, 1997.
13. Houston M, Estevez M, Chumley P, Aslan M, Marklund S, Parks D, Freeman BA. Binding of xanthine oxidase to vascular endothelium. Kinetic characterization and oxidative impairment of nitric oxide-dependent signaling. J Biol Chem 274: 4985-4994, 1999.
14. Karplus M, McCammon JA. The internal dynamics of globular proteins. CRC Crit Rev Biochem 9: 293-349, 1981.
15. Lu HP, Xun L, Xie XS. Single-molecule enzymatic dynamics. Science 282: 1877-1882, 1998.
16. Michaelis L, Menten ML. Die Kinetik der Invertinwirkung. Biochem Z 49: 333-369, 1913.
17. Platt JR. Strong inference. Science 146: 347-353, 1964.
18. Schwartz LM, Bukowski TR, Revkin JH, Bassingthwaighte JB. Cardiac endothelial transport and metabolism of adenosine and inosine. Am J Physiol Heart Circ Physiol 277: H1241-H1251, 1999.
19. van Oijen AM, Blainey PC, Crampton DJ, Richardson CC, Ellenberger T, Xie XS. Single-molecule kinetics of lambda exonuclease reveal base dependence and dynamic disorder. Science 301: 1235-1238, 2003.
20. Yang S, Cao J, Silbey RJ, Sung J. Quantitative interpretation of the randomness in single enzyme turnover times. Biophys J 101: 519-524, 2011.