Microinjection of mRNA or morpholinos for reverse genetic analysis in the starlet sea anemone, Nematostella vectensis

Nature Protocols

Volumn 8, Issue 5, 2013, Pages 924-934

  Layden, Michael J ^a   Röttinger, Eric ^a   Wolenski, Francis S ^b   Gilmore, Thomas D ^b   Martindale, Mark Q ^a  

^a UNIVERSITY OF FLORIDA   (United States)

^b Boston University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; MESSENGER RNA; MORPHOLINO OLIGONUCLEOTIDE; POLYSTYRENE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CLINICAL PROTOCOL; EMBRYO; EXPERIMENTAL DESIGN; GENE FUNCTION; GENE IDENTIFICATION; GENE OVEREXPRESSION; GENETIC ANALYSIS; GENOME ANALYSIS; IN VITRO STUDY; MICROINJECTION; MICROSCOPY; MOLECULAR CLONING; NEMATOSTELLA VECTENSIS; NONHUMAN; PRIORITY JOURNAL; REVERSE GENETICS; RNA TRANSCRIPTION; RNA TRANSLATION;

ANIMALS; EMBRYO, NONMAMMALIAN; MICROINJECTIONS; MORPHOLINOS; REVERSE GENETICS; RNA, MESSENGER; SEA ANEMONES;

ACTINIARIA; NEMATOSTELLA; NEMATOSTELLA VECTENSIS;

EID: 84877058768     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.009     Document Type: Article

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