Radiation damage in macromolecular crystallography: What is it and why do we care?

NATO Science for Peace and Security Series A: Chemistry and Biology

Volumn , Issue , 2013, Pages 69-77

  Garman, Elspeth F ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Cryocrystallography; Diffraction; Dose; Radiation damage

Indexed keywords

EID: 84877040567     PISSN: 18746489     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-94-007-6232-9_7     Document Type: Article

References (23)

1. Blake C, Phillips DC (1962) Effects of X-irradiation on single crystals of myoglobin in proceedings of the symposium on the biological effects of ionizing radiation at the molecular level. International Atomic Energy Agency, Vienna, pp 183-191
2. Blundell T, Johnson LN (1976) Protein crystallography. Academic, New York
3. Burmeister WP (2000) Structural changes in a cryo-cooled protein crystal owing to radiation damage. Acta Crystallogr D 56:328-341
4. Cosier J, Glazer AM (1986) A nitrogen-gas-stream cryostat for general X-ray diffraction studies. J Appl Crystallogr 19:105-107
5. Crick FHC, Magdoff BS (1956) The theory of the method of isomorphous replacement for protein crystals. I. Acta Crystallogr 9:901-908
6. Diederichs K (2006) Some aspects of quantitative analysis and correction of radiation damage. Acta Crystallogr D 62:96-101
7. Garman E (1999) Cool data: quantity AND quality. Acta Crystallogr D 55:1641-1653
8. Garman EF (2010) Radiation damage in macromolecular crystallography: what is it and why should we care? Acta Crystallogr D 66:339-351
9. Garman EF, Schneider TR (1997) Macromolecular cryocrystallography. J Appl Crystallogr 30:211-237
10. Helliwell JR (1988) Protein crystal perfection and the nature of radiation damage. J Crystallogr Growth 90:259-272
11. Holton JM (2009) A beginner's guide to radiation damage. J Synchrotron Rad 16:133-142
12. Kmetko J, Husseini NS, Naides M, Kalinin Y, Thorne RE (2006) Quantifying X-ray radiation damage in protein crystals at cryogenic temperatures. Acta Crystallogr D 62:1030-1038
13. Meents A, Gutmann S, Wagner A, Schulze-Briese C (2010) Origin and temperature dependence of radiation damage in biological samples at cryogenic temperatures. Proc Natl Acad Sci U S A 107:1094-1099
14. Murray JM, Garman EF (2002) Investigation of possible free-radical scavengers and metrics for radiation damage in protein cryocrystallography. J Synchrotron Rad 9(6):347-354
15. Murray JW, Garman EF, Ravelli RBG (2004) X-ray absorption by macromolecular crystals: the effects of wavelength and crystal composition on absorbed dose. J Appl Crystallogr 37: 513-522
16. Nave C, Garman EF (2005) Towards an understanding of radiation damage in cryocooled macromolecular crystals. J Synchrotron Rad 12:257-260
17. Owen RL, Rudino-Pinera E, Garman EF (2006) Experimental determination of the radiation dose limit for cryocooled protein crystals. Proc Natl Acad Sci U S A 103:4912-4917
18. Paithankar KS, Owen RL, Garman EF (2009) Absorbed dose calculations for macromolecular crystals: improvements to RADDOSE. J Synchrotron Rad 16:152-162
19. Ravelli RBG, Garman E (2006) Radiation damage in macromolecular cryocrystallography. Curr Opin Struct Biol 16:24-629
20. Ravelli RBG, McSweeney S (2000) The 'fingerprint' that X-rays can leave on structures. Structure 8:315-328
21. Rodgers DW (1997) Practical cryocrystallography. Methods Enzymol 276:183-203
22. Teng T-Y (1990) Mounting of crystals for macromolecular crystallography in a freestanding thin-film. J Appl Crystallogr 23:387-391
23. Weik M, Ravelli RBG, Kryger G, McSweeney S, Raves ML, Harel M, Gros P, Silman I, Kroon J, Sussman JL (2000) Specific chemical and structural damage to proteins produced by synchrotron radiation. Proc Natl Acad Sci U S A 97:623-628