High glucose-induced apoptosis in cultured podocytes involves TRPC6-dependent calcium entry via the RhoA/ROCK pathway

Biochemical and Biophysical Research Communications

Volumn 434, Issue 2, 2013, Pages 394-400

  Yang, He ^a   Zhao, Bo ^a   Liao, Chang ^a   Zhang, Rui ^a   Meng, Kexin ^a   Xu, Jia ^a   Jiao, Jundong ^a  

^a HARBIN MEDICAL UNIVERSITY   (China)

Author keywords

Apoptosis; Podocytes; RhoA GTPase; ROS; TRPC6

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; GLUCOSE; HYDROGEN PEROXIDE; REACTIVE OXYGEN METABOLITE; RHO KINASE; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; SMALL INTERFERING RNA; TRANSIENT RECEPTOR POTENTIAL CHANNEL 1; TRANSIENT RECEPTOR POTENTIAL CHANNEL 5; TRANSIENT RECEPTOR POTENTIAL CHANNEL 6;

APOPTOSIS; ARTICLE; ENZYME ACTIVITY; ENZYME INHIBITION; GLUCOSE BLOOD LEVEL; HUMAN; HUMAN CELL; PODOCYTE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

AMIDES; APOPTOSIS; CALCIUM; CALCIUM SIGNALING; CELLS, CULTURED; ENZYME ACTIVATION; GENE KNOCKDOWN TECHNIQUES; GLUCOSE; HUMANS; IN SITU NICK-END LABELING; PODOCYTES; PYRIDINES; REACTIVE OXYGEN SPECIES; RHOA GTP-BINDING PROTEIN; RNA, MESSENGER; RNA, SMALL INTERFERING; TRPC CATION CHANNELS;

EID: 84877017382     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2013.03.087     Document Type: Article

References (26)

1. Pagtalunan M.E., Miller P.L., Jumping-Eagle S., Nelson R.G., Myers B.D., Rennke H.G., Coplon N.S., Sun L., Meyer T.W. Podocyte loss and progressive glomerular injury in type II diabetes. J. Clin. Invest. 1997, 99:342-348.
2. Meyer T.W., Bennett P.H., Nelson R.G. Podocyte number predicts long-term urinary albumin excretion in Pima Indians with Type II diabetes and microalbuminuria. Diabetologia 1999, 42:1341-1344.
3. Eid A.A., Gorin Y., Fagg B.M., Maalouf R., Barnes J.L., Block K., Abboud H.E. Mechanisms of podocyte injury in diabetes: role of cytochrome P450 and NADPH oxidases. Diabetes 2009, 58:1201-1211.
4. Susztak K., Raff A.C., Schiffer M., Bottinger E.P. Glucose-induced reactive oxygen species cause apoptosis of podocytes and podocyte depletion at the onset of diabetic nephropathy. Diabetes 2006, 55:225-233.
5. Wang W., Wang Y., Long J., Wang J., Haudek S.B., Overbeek P., Chang B.H., Schumacker P.T., Danesh F.R. Mitochondrial fission triggered by hyperglycemia is mediated by ROCK1 activation in podocytes and endothelial cells. Cell Metab. 2012, 15:186-200.
6. Dryer S.E., Reiser J. TRPC6 channels and their binding partners in podocytes: role in glomerular filtration and pathophysiology. Am. J. Physiol. Renal Physiol. 2010, 299:F689-F701.
7. Reiser J., Polu K.R., Moller C.C., Kenlan P., Altintas M.M., Wei C., Faul C., Herbert S., Villegas I., Avila-Casado C., McGee M., Sugimoto H., Brown D., Kalluri R., Mundel P., Smith P.L., Clapham D.E., Pollak M.R. TRPC6 is a glomerular slit diaphragm-associated channel required for normal renal function. Nat. Genet. 2005, 37:739-744.
8. Moller C.C., Wei C., Altintas M.M., Li J., Greka A., Ohse T., Pippin J.W., Rastaldi M.P., Wawersik S., Schiavi S., Henger A., Kretzler M., Shankland S.J., Reiser J. Induction of TRPC6 channel in acquired forms of proteinuric kidney disease. J. Am. Soc. Nephrol. 2007, 18:29-36.
9. Hofmann T., Obukhov A.G., Schaefer M., Harteneck C., Gudermann T., Schultz G. Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol. Nature 1999, 397:259-263.
10. Worley P.F., Zeng W., Huang G.N., Yuan J.P., Kim J.Y., Lee M.G., Muallem S. TRPC channels as STIM1-regulated store-operated channels. Cell Calcium 2007, 42:205-211.
11. Kim E.Y., Anderson M., Dryer S.E. Sustained activation of N-methyl-d-aspartate receptors in podoctyes leads to oxidative stress, mobilization of transient receptor potential canonical 6 channels, nuclear factor of activated T cells activation, and apoptotic cell death. Mol. Pharmacol. 2012, 82:728-737.
12. Graham S., Ding M., Ding Y., Sours-Brothers S., Luchowski R., Gryczynski Z., Yorio T., Ma H., Ma R. Canonical transient receptor potential 6 (TRPC6), a redox-regulated cation channel. J. Biol. Chem. 2010, 285:23466-23476.
13. Tian D., Jacobo S.M., Billing D., Rozkalne A., Gage S.D., Anagnostou T., Pavenstaedt H., Hsu H.H., Schlondorff J., Ramos A., Greka A. Antagonistic regulation of actin dynamics and cell motility by TRPC5 and TRPC6 channels. Sci. Signal. 2010, 3:ra77.
14. Greka A., Mundel P. Balancing calcium signals through TRPC5 and TRPC6 in podocytes. J. Am. Soc. Nephrol. 2011, 22:1969-1980.
15. Zhu L., Jiang R., Aoudjit L., Jones N., Takano T. Activation of RhoA in podocytes induces focal segmental glomerulosclerosis. J. Am. Soc. Nephrol. 2011, 22:1621-1630.
16. Wang L., Ellis M.J., Gomez J.A., Eisner W., Fennell W., Howell D.N., Ruiz P., Fields T.A., Spurney R.F. Mechanisms of the proteinuria induced by Rho GTPases. Kidney Int. 2012, 81:1075-1085.
17. Zhang H., Ding J., Fan Q., Liu S. TRPC6 up-regulation in Ang II-induced podocyte apoptosis might result from ERK activation and NF-kappaB translocation. Exp. Biol. Med. (Maywood) 2009, 234:1029-1036.
18. Chen S., He F.F., Wang H., Fang Z., Shao N., Tian X.J., Liu J.S., Zhu Z.H., Wang Y.M., Wang S., Huang K., Zhang C. Calcium entry via TRPC6 mediates albumin overload-induced endoplasmic reticulum stress and apoptosis in podocytes. Cell Calcium 2011, 50:523-529.
19. Zhang R., Sun H., Liao C., Yang H., Zhao B., Tian J., Dong S., Zhang Z., Jiao J. Chronic hypoxia in cultured human podocytes inhibits BKCa channels by upregulating its beta4-subunit. Biochem. Biophys. Res. Commun. 2012, 420:505-510.
20. Liao C., Yang H., Zhang R., Sun H., Zhao B., Gao C., Zhu F., Jiao J. The upregulation of TRPC6 contributes to Ca(2)(+) signaling and actin assembly in human mesangial cells after chronic hypoxia. Biochem. Biophys. Res. Commun. 2012, 421:750-756.
21. Thilo F., Liu Y., Loddenkemper C., Schuelein R., Schmidt A., Yan Z., Zhu Z., Zakrzewicz A., Gollasch M., Tepel M. VEGF regulates TRPC6 channels in podocytes. Nephrol. Dial. Transplant. 2012, 27:921-929.
22. Kim E.Y., Anderson M., Dryer S.E. Insulin increases surface expression of TRPC6 channels in podocytes: role of NADPH oxidases and reactive oxygen species. Am. J. Physiol. Renal Physiol. 2012, 302:F298-F307.
23. Wang Z., Wei X., Zhang Y., Ma X., Li B., Zhang S., Du P., Zhang X., Yi F. NADPH oxidase-derived ROS contributes to upregulation of TRPC6 expression in puromycin aminonucleoside-induced podocyte injury. Cell. Physiol. Biochem. 2009, 24:619-626.
24. Dietrich A., Mederos Y.S., Gollasch M., Gross V., Storch U., Dubrovska G., Obst M., Yildirim E., Salanova B., Kalwa H., Essin K., Pinkenburg O., Luft F.C., Gudermann T., Birnbaumer L. Increased vascular smooth muscle contractility in TRPC6-/- mice. Mol. Cell Biol. 2005, 25:6980-6989.
25. Li Z., Xu J., Xu P., Liu S., Yang Z. Wnt/beta-catenin signalling pathway mediates high glucose induced cell injury through activation of TRPC6 in podocytes. Cell Prolif. 2013, 46:76-85.
26. Wang L., Chang J.H., Paik S.Y., Tang Y., Eisner W., Spurney R.F. Calcineurin (CN) activation promotes apoptosis of glomerular podocytes both in vitro and in vivo. Mol. Endocrinol. 2011, 25:1376-1386.