Poly(N-isopropylacrylamide)-coated microwell arrays for construction and recovery of multicellular spheroids

Journal of Bioscience and Bioengineering

Volumn 115, Issue 6, 2013, Pages 695-699

  Shimizu, Kazunori ^a,b   Kusamori, Kosuke ^a   Nishikawa, Makiya ^a   Mizuno, Narumi ^a   Nishikawa, Tomoko ^a   Masuzawa, Akira ^a   Katano, Shingo ^b   Takahashi, Yuki ^a   Takakura, Yoshinobu ^a   Konishi, Satoshi ^a,b  

^a KYOTO UNIVERSITY   (Japan)

^b RITSUMEIKAN UNIVERSITY   (Japan)

Author keywords

BioMEMS; Microdevice; Multicellular aggregates; PNIPAAm; Thermoresponsive polymer

Indexed keywords

EFFECTS OF TEMPERATURE; LOWER CRITICAL SOLUTION TEMPERATURE; MICRO-DEVICE; MULTICELLULAR AGGREGATES; PNIPAAM; POLY-N-ISOPROPYL ACRYLAMIDE; SOLVENT CASTING METHOD; THERMORESPONSIVE POLYMER;

ACRYLIC MONOMERS; BIOMEMS; CELL CULTURE; MOLECULAR BIOLOGY; RECOVERY;

MICROCHANNELS;

ALBUMIN; POLY(N ISOPROPYLACRYLAMIDE);

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CELL ADHESION; DISSOLUTION; FUNCTIONAL ASSESSMENT; GEOMETRY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; MICROARRAY ANALYSIS; MOUSE; MULTICELLULAR SPHEROID; NONHUMAN; PHASE CONTRAST MICROSCOPY;

ACRYLAMIDES; ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; HUMANS; MICE; NIH 3T3 CELLS; POLYMERS; SPHEROIDS, CELLULAR; TEMPERATURE;

EID: 84876996682     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2012.12.017     Document Type: Article

References (32)

1. Torisawa Y.S., Takagi A., Nashimoto Y., Yasukawa T., Shiku H., Matsue T. A multicellular spheroid array to realize spheroid formation, culture, and viability assay on a chip. Biomaterials 2007, 28:559-566.
2. Kloss D., Fischer M., Rothermel A., Simon J.C., Robitzki A.A. Drug testing on 3D in vitro tissues trapped on a microcavity chip. Lab Chip 2008, 8:879-884.
3. Kunz-Schughart L.A., Freyer J.P., Hofstaedter F., Ebner R. The use of 3-D cultures for high-throughput screening: the multicellular spheroid model. J. Biomol. Screen. 2004, 9:273-285.
4. Zhang X.L., Wang W., Yu W.T., Xie Y.B., Zhang X.H., Zhang Y., Ma X.J. Development of an in vitro multicellular tumor spheroid model using microencapsulation and its application in anticancer drug screening and testing. Biotechnol. Prog. 2005, 21:1289-1296.
5. Krohn A., Song Y.H., Muehlberg F., Droll L., Beckmann C., Alt E. CXCR4 receptor positive spheroid forming cells are responsible for tumor invasion in vitro. Cancer Lett. 2009, 280:65-71.
6. Okochi M., Takano S., Isaji Y., Senga T., Hamaguchi M., Honda H. Three-dimensional cell culture array using magnetic force-based cell patterning for analysis of invasive capacity of BALB/3T3/v-src. Lab Chip 2009, 9:3378-3384.
7. Hsieh C.-H., Wang J.-L., Huang Y.-Y. Large-scale cultivation of transplantable dermal papilla cellular aggregates using microfabricated PDMS arrays. Acta Biomater. 2011, 7:315-324.
8. Mironov V., Visconti R.P., Kasyanov V., Forgacs G., Drake C.J., Markwald R.R. Organ printing: tissue spheroids as building blocks. Biomaterials 2009, 30:2164-2174.
9. Jakab K., Norotte C., Damon B., Marga F., Neagu A., Besch-Williford C.L., Kachurin A., Church K.H., Park H., Mironov V., other 3 authors Tissue engineering by self-assembly of cells printed into topologically defined structures. Tissue Eng. Part A 2008, 14:413-421.
10. Metzger W., Sossong D., Bachle A., Putz N., Wennemuth G., Pohlemann T., Oberringer M. The liquid overlay technique is the key to formation of co-culture spheroids consisting of primary osteoblasts, fibroblasts and endothelial cells. Cytotherapy 2011, 13:1000-1012.
11. Tung Y.C., Hsiao A.Y., Allen S.G., Torisawa Y.S., Ho M., Takayama S. High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array. Analyst 2011, 136:473-478.
12. Okubo H., Matsushita M., Kamachi H., Kawai T., Takahashi M., Fujimoto T., Nishikawa T., Todo S. Novel method for faster formation of rat liver cell spheroids. Artif. Organs 2002, 26:497-505.
13. Frith J.E., Thomson B., Genever P.G. Dynamic three-dimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential. Tissue Eng. Part C Methods 2010, 16:735-749.
14. Wong S.F., No D.Y., Choi Y.Y., Kim D.S., Chung B.G., Lee S.-H. Concave microwell based size-controllable hepatosphere as a three-dimensional liver tissue model. Biomaterials 2011, 32:8087-8096.
15. Karp J.M., Yeh J., Eng G., Fukuda J., Blumling J., Suh K.-Y., Cheng J., Mahdavi A., Borenstein J., Langer R., Khademhosseini A. Controlling size, shape and homogeneity of embryoid bodies using poly(ethylene glycol) microwells. Lab. Chip 2007, 7:786-794.
16. Sakai Y., Tanaka T., Fukuda J., Nakazawa K. Alkoxyresorufin O-dealkylase assay using a rat hepatocyte spheroid microarray. J. Biosci. Bioeng. 2010, 109:395-399.
17. Nakazawa K., Izumi Y., Fukuda J., Yasuda T. Hepatocyte spheroid culture on a polydimethylsiloxane chip having microcavities. J. Biomater. Sci. Polym. Ed. 2006, 17:859-873.
18. Fukuda J., Nakazawa K. Orderly arrangement of hepatocyte spheroids on a microfabricated chip. Tissue Eng. 2005, 11:1254-1262.
19. Inaba R., Khademhosseini A., Suzuki H., Fukuda J. Electrochemical desorption of self-assembled monolayers for engineering cellular tissues. Biomaterials 2009, 30:3573-3579.
20. Anada T., Masuda T., Honda Y., Fukuda J., Arai F., Fukuda T., Suzuki O. Three-dimensional cell culture device utilizing thin membrane deformation by decompression. Sens. Actuators B Chem. 2010, 147:376-379.
21. Moeller H.-C., Mian M.K., Shrivastava S., Chung B.G., Khademhosseini A. A microwell array system for stem cell culture. Biomaterials 2008, 29:752-763.
22. Tekin H., Anaya M., Brigham M.D., Nauman C., Langer R., Khademhosseini A. Stimuli-responsive microwells for formation and retrieval of cell aggregates. Lab Chip 2010, 10:2411-2418.
23. Takezawa T., Mori Y., Yoshizato K. Cell culture on a thermo-responsive polymer surface. Biotechnol. (N. Y.) 1990, 8:854-856.
24. Moran M.T., Carroll W.M., Gorelov A., Rochev Y. Intact endothelial cell sheet harvesting from thermoresponsive surfaces coated with cell adhesion promoters. J. R. Soc. Interface 2007, 4:1151-1157.
25. Shimizu K., Fujita H., Nagamori E. Oxygen Plasma-Treated Thermoresponsive polymer surfaces for cell sheet engineering. Biotechnol. Bioeng. 2010, 106:303-310.
26. Ota H., Kodama T., Miki N. Rapid formation of size-controlled three dimensional hetero-cell aggregates using micro-rotation flow for spheroid study. Biomicrofluidics 2011, 5:34105.
27. Nash M.E., Carroll W.M., Nikoloskya N., Yang R., Connell C.O., Gorelov A.V., Dockery P., Liptrot C., Lyng F.M., Garcia A., Rochev Y.A. Straightforward, one-step fabrication of ultrathin thermoresponsive films from commercially available pNIPAm for cell culture and recovery. ACS Appl. Mater. Interfaces 2011, 3:1980-1990.
28. Fujita H., Shimizu K., Nagamori E. Application of a cell sheet-polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability. Biotechnol. Bioeng. 2009, 103:370-377.
29. Shimizu K., Sasaki H., Hida H., Fujita H., Obinata K., Shikida M., Nagamori E. Assembly of skeletal muscle cells on a Si-MEMS device and their generative force measurement. Biomed. Microdevices 2010, 12:247-252.
30. Wang D., Cheng D., Guan Y., Zhang Y. Thermoreversible hydrogel for in situ generation and release of HepG2 spheroids. Biomacromolecules 2011, 12:578-584.
31. Lee J., Cuddihy M.J., Cater G.M., Kotov N.A. Engineering liver tissue spheroids with inverted colloidal crystal scaffolds. Biomaterials 2009, 30:4687-4694.
32. Malonne H., Eeckman F., Fontaine D., Otto A., Vos L.D., Moes A., Fontaine J., Amighi K. Preparation of poly(N-isopropylacrylamide) copolymers and preliminary assessment of their acute and subacute toxicity in mice. Eur. J. Pharm. Biopharm. 2005, 61:188-194.