Structural analysis of flagellar axonemes from inner arm dynein knockdown strains of Trypanosoma brucei

Biocell

Volumn 36, Issue 3, 2012, Pages 133-141

  Zukas, Randi ^a   Chang, Alex J ^a   Rice, Marian ^b   Springer, Amy L ^a  

^a AMHERST COLLEGE   (United States)

^b MOUNT HOLYOKE COLLEGE   (United States)

Author keywords

Central pair; Flagellar motility; Transmission electron microscopy

Indexed keywords

AXONEMAL DYNEIN; DYNEIN F; PROTOZOAL DNA; PROTOZOAL PROTEIN; RNA; UNCLASSIFIED DRUG;

ARTICLE; AXONEME; CONTROLLED STUDY; GENE SILENCING; NONHUMAN; PROTEIN FUNCTION; RNA INTERFERENCE; STRUCTURE ANALYSIS; TRANSMISSION ELECTRON MICROSCOPY; TRYPANOSOMA BRUCEI;

ANIMALS; AXONEME; CELL CYCLE; CELL MOVEMENT; DYNEINS; FLAGELLA; MICROSCOPY, ELECTRON, TRANSMISSION; MODELS, BIOLOGICAL; RNA INTERFERENCE; TRYPANOSOMA BRUCEI BRUCEI;

MASTIGOPHORA (FLAGELLATES); PROTOZOA; TRYPANOSOMA BRUCEI;

EID: 84876759976     PISSN: 03279545     EISSN: 16675746     Source Type: Journal    
DOI: None     Document Type: Article

References (35)

1. Abramoff MD, Magalhães PJ, Ram SJ (2004). Image processing with ImageJ. Biophotonics International 11: 36-42.
2. Baron DM, Kabututu ZP, Hill KL (2007a). Stuck in reverse: loss of LC1 in Trypanosoma brucei disrupts outer dynein arms and leads to reverse flagellar beat and backward movement. Journal of Cell Science 120: 1513-1520.
3. Baron DM, Ralston KS, Kabututu ZP, Hill KL (2007b). Functional genomics in Trypanosoma brucei identifies evolutionarily conserved components of motile flagella. Journal of Cell Science 120: 478-491.
4. Bastin P, Matthews KR, Gull K (1996). The paraflagellar rod of kinetoplastida: solved and unsolved questions. Parasitology Today 12: 302-307.
5. Bower R, VanderWaal K, O'Toole E, et al. (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Molecular Biology of the Cell 20: 3055-3063.
6. Branche C, Kohl L, Toutirais G, Buisson, J, Cosson J, Bastin P (2006) Conserved and specific functions of axoneme components in trypanosome motility. Journal of Cell Science 119: 3443-3455.
7. Broadhead R, Dawe HR, Farr H, Griffiths S, Hart SR, Portman N, Shaw MK, Ginger ML, Gaskell SJ, McKean PG, Gull K (2006) Flagellar motility is required for the viability of the bloodstream trypanosome. Nature 440: 224-227.
8. Brun R, Schönenberger, M (1979) Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei in a semidefined medium. Acta Tropica 36: 289-292.
9. Bui KH, Sakakibara H, Movassagh T, Oiwa K, Ishikawa T (2008) Molecular architecture of inner dynein arms in situ in Chlamydomonas reinhardtii flagella. Journal of Cell Biology 183: 923-932.
10. Chandler J, Vandoros AV, Mozeleski B, Klingbeil MM (2008) Stemloop silencing reveals that a third mitochondrial DNA polymerase, POLID, is required for kinetoplast DNA replication in trypanosomes. Eukaryotic Cell 7: 2141-2146.
11. Daniel WW (2000) Applied Nonparametric Statistics. Duxbury Press, Pacific Grove, CA.
12. Gadelha C, Wickstead B, de Souza W, Gull K, Cunha-e-Silva N (2005) Cryptic paraflagellar rod in endosymbiont-containing kinetoplastid protozoa. Eukaryotic Cell 4: 516-525.
13. Garcia A, Courtin D, Solano P, Koffi M, Jamonneau V (2006) Human African trypanosomiasis: connecting parasite and host genetics. Trends in Parasitology 22: 405-409.
14. Gennerich A, Vale RD (2009) Walking the walk: how kinesin and dynein coordinate their steps. Current Opinion in Cell Biology 21: 59-67.
15. Gull K (2003) Host-parasite interactions and trypanosome morphogenesis: a flagellar pocketful of goodies. Current Opinion in Microbiology 6: 365-370.
16. Holzbaur ELF, Vallee RB (1994) Dyneins: Molecular Structure and Cellular Function. Annual Review of Cell Biology 10: 339-372.
17. Ishikawa T, Sakakibara H, Oiwa K (2007) The architecture of outer dynein arms in situ. Journal of Molecular Biology 368: 1249-1258.
18. Kamiya R, Kurimoto E, Muto E (1991) Two types of Chlamydomonas flagellar mutants missing different components of innerarm dynein. The Journal of Cell Biology 112: 441-447.
19. Kotani N, Sakakibara H, Burgess SA, Kojima H, Oiwa K (2007) Mechanical properties of inner-arm dynein-f (dynein I1) studied with in vitro motility assays. Biophysical Journal 93: 886-894.
20. Lindemann CB, Lesich KA (2010) Flagellar and ciliary beating: the proven and the possible. Journal of Cell Science 123: 519-528.
21. Myster SH, Knott JA, O'Toole E, Porter ME (1997) The Chlamydomonas Dhc1 gene encodes a dynein heavy chain subunit required for assembly of the I1 inner arm complex. Molecular Biology of the Cell 8: 607-620.
22. Oberholzer M, Lopez MA, Ralston KS, Hill KL (2009) Approaches for functional analysis of flagellar proteins in African trypanosomes. Methods in Cell Biology 93: 21-57.
23. Porter ME, Sale WS (2000) The 9 + 2 axoneme anchors multiple inner arm dyneins and a network of kinases and phosphatases that control motility. Journal of Cell Biology 151: F37-42.
24. Ralston KS, Hill KL (2008) The flagellum of Trypanosoma brucei: new tricks from an old dog. International Journal for Parasitology 38: 869-884.
25. Ralston KS, Kabututu ZP, Melehani JH, Oberholzer M, Hill KL (2009) The Trypanosoma brucei flagellum: moving parasites in new directions. Annual Review of Microbiology 63: 335-362.
26. Ralston KS, Lerner AG, Diener DR, Hill KL (2006) Flagellar motility contributes to cytokinesis in Trypanosoma brucei and is modulated by an evolutionarily conserved dynein regulatory system. Eukaryotic Cell 5: 696-711.
27. Robinson D, Beattie P, Sherwin T, Gull K (1991) Microtubules, tubulin, and microtubule-associated proteins of trypanosomes. Methods in Enzymology 196: 285-299.
28. Rodríguez JA, Lopez MA, Thayer MC, Zhao Y, Oberholzer M, Chang DD, Kisalu NK, Penichet ML, Helguera G, Bruinsma, R, Hill KL, Miao J (2009) Propulsion of African trypanosomes is driven by bihelical waves with alternating chirality separated by kinks. Proceedings of the National Academy of Sciences (USA) 106: 19322-19327.
29. Springer AL, Bruhn DF, Kinzel KW, Rosenthal NF, Zukas R, Klingbeil MM (2011) Silencing of a putative inner arm dynein heavy chain results in flagellar immotility in Trypanosoma brucei. Molecular and Biochemical Parasitology 175: 68-75.
30. Steverding D (2010) The development of drugs for treatment of sleeping sickness: a historical review. Parasites & Vectors 3: 15.
31. Wickstead B, Gull K (2007) Dyneins across eukaryotes: a comparative genomic analysis. Traffic 8: 1708-1721.
32. Wild CJ, Seber GAF (1999) Chance Encounters: A First Course in Data Analysis and Inference. John Wiley, New York.
33. Wilson-Leedy JG, Ingermann RL (2007) Development of a novel CASA system based on open source software for characterization of zebrafish sperm motility parameters. Theriogenology 67: 661-672.
34. Wirschell M, Hendrickson T, Sale WS (2007) Keeping an eye on I1: I1 dynein as a model for flagellar dynein assembly and regulation. Cell Motility and the Cytoskeleton 64: 569-579.
35. Wirtz E, Leal S, Ochatt C, Cross GA (1999) A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei. Molecular and Biochemical Parasitology 99: 89-101.