Use of uridine auxotrophy (ura3) for markerless transformation of the mycoinsecticide Beauveria bassiana

Applied Microbiology and Biotechnology

Volumn 97, Issue 7, 2013, Pages 3017-3025

  Ying, Sheng Hua ^a,b   Feng, Ming Guang ^a   Keyhani, Nemat O ^b  

^a ZHEJIANG UNIVERSITY   (China)

^b UNIVERSITY OF FLORIDA   (United States)

Author keywords

Insect biological control; Keyword: Beauveria bassiana; Markerless transformation; Uridine auxotrophy

Indexed keywords

AUXOTROPHY; BEAUVERIA BASSIANA; BIOLOGICAL CONTROLS; ENTOMOPATHOGENIC FUNGUS; MARKERLESS; SCREENING PROTOCOL; TRANSFORMATION PROTOCOLS; TRANSFORMATION SYSTEMS;

GENETIC ENGINEERING; RESTORATION;

GENES;

INSECTICIDE; MYCOINSECTICIDE; OROTIDINE 5' PHOSPHATE DECARBOXYLASE; UNCLASSIFIED DRUG; URIDINE;

BIOLOGICAL CONTROL; ENTOMOPATHOGENIC ORGANISM; FUNGUS; GENETIC ENGINEERING; GENETICALLY MODIFIED ORGANISM; INSECTICIDE; MUTATION; NUCLEIC ACID; PLASMID; SCAVENGING (CHEMISTRY); TRANSFORMATION; VIRULENCE;

ARTICLE; ASEXUAL SPORE; AUTOTROPHY; AUXOTROPHY; BEAUVERIA BASSIANA; BIOASSAY; BIOLOGICAL PEST CONTROL; DELETION MUTANT; EXPRESSED SEQUENCE TAG; FUNGAL STRAIN; FUNGAL VIRULENCE; FUNGUS ISOLATION; GENE DELETION; GENE DISRUPTION; MYCELIUM; NONHUMAN; PHENOTYPE; PLASMID; SOUTHERN BLOTTING; VEGETATIVE GROWTH; WILD TYPE;

BEAUVERIA; GENE DELETION; GENE TRANSFER TECHNIQUES; GENETIC ENGINEERING; GENETIC VECTORS; GENETICS, MICROBIAL; MOLECULAR BIOLOGY; PLASMIDS; SELECTION, GENETIC; TRANSFORMATION, GENETIC; URIDINE;

CORDYCEPS BASSIANA; FUNGI; HEXAPODA;

EID: 84876680905     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4426-0     Document Type: Article

References (36)

1. Berges T, Barreau C (1991) Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes. Curr Genet 19:359-365
2. Cho EM, Boucias D, Keyhani NO (2006a) EST analysis of cDNA libraries from the entomopathogenic fungus Beauveria (Cordyceps) bassiana. II. Fungal cells sporulating on chitin and producing oosporein. Microbiology 152:2855-2864
3. Cho EM, Liu L, Farmerie W, Keyhani NO (2006b) EST analysis of cDNA libraries from the entomopathogenic fungus Beauveria (Cordyceps) bassiana. I. Evidence for stage-specific gene expression in aerial conidia, in vitro blastospores and submerged conidia. Microbiology 152:2843-2854
4. Cole MF, Bowen WH, Zhao XJ, Cihlar RL (1995) Avirulence of Candida albicans auxotrophic mutants in a rat model of oropharyngeal candidiasis. FEMS Microbiol Lett 126:177-180
5. D'Enfert C, Diaquin M, Delit A, Wuscher N, Debeaupuis JP, Huerre M, Latge JP (1996) Attenuated virulence of uridine-uracil auxotrophs of Aspergillus fumigatus. Infect Immun 64:4401-4405
6. Fan Y, Borovsky D, Hawkings C, Ortiz-Urquiza A, Keyhani NO (2012a) Exploiting host molecules to augment mycoinsecticide virulence. Nat Biotechnol 30:35-37. doi: 10.1038/nbt.2080
7. Fan Y, Pereira RM, Kilic E, Casella G, Keyhani NO (2012b) Pyrokinin β-neuropeptide affects necrophoretic behavior in fire ants (S. invicta), and expression of β-NP in a mycoinsecticide increases its virulence. PLoS One 7:e26924. doi: 10.1371/journal.pone.0026924
8. Fan Y, Zhang S, Kruer N, Keyhani NO (2011) High-throughput insertion mutagenesis and functional screening in the entomopathogenic fungus Beauveria bassiana. J Invertebr Pathol 106:274-279
9. Fang W, Zhang Y, Yang X, Zheng X, Duan H, Li Y, Pei Y (2004) Agrobacterium tumefaciens-mediated transformation of Beauveria bassiana using an herbicide resistance gene as a selection marker. J Invertebr Pathol 85:18-24
10. Fang WG, Feng J, Fan YH, Zhang YJ, Bidochka MJ, Leger RJS, Pei Y (2009) Expressing a fusion protein with protease and chitinase activities increases the virulence of the insect pathogen Beauveria bassiana. J Invertebr Pathol 102:155-159
11. Fernandes EKK, Rangel DEN, Moraes AML, Bittencourt VREP, Roberts DW (2007) Variability in tolerance to UV-B radiation among Beauveria spp. isolates. J Invertebr Pathol 96:237-243
12. Glare T, Caradus J, Gelernter W, Jackson T, Keyhani N, Kohl J, Marrone P, Morin L, Stewart A (2012) Have biopesticides come of age? Trends Biotechnol 30:250-258. doi: 10.1016/j.tibtech.2012.01.003
13. Gouka RJ, Hessing JG, Stam H, Musters W, van den Hondel CA (1995) A novel strategy for the isolation of defined pyrG mutants and the development of a site-specific integration system for Aspergillus awamori. Curr Genet 27:536-540
14. Hajek AE, Delalibera I (2010) Fungal pathogens as classical biological control agents against arthropods. Biocontrol 55:147-158
15. Hartl L, Seiboth B (2005) Sequential gene deletions in Hypocrea jecorina using a single blaster cassette. Curr Genet 48:204-211. doi: 10.1007/s00294-005-0011-8
16. Holder DJ, Keyhani NO (2005) Adhesion of the entomopathogenic fungus Beauveria (Cordyceps) bassiana to substrata. Appl Environ Microbiol 71:5260-5266
17. Jackson MA, Dunlap CA, Jaronski ST (2010) Ecological considerations in producing and formulating fungal entomopathogens for use in insect biocontrol. Biocontrol 55:129-145
18. Jaronski ST (2010) Ecological factors in the inundative use of fungal entomopathogens. Biocontrol 55:159-185
19. Jin K, Zhang YJ, Fang WG, Luo ZB, Zhou YH, Pei Y (2010) Carboxylate transporter gene JEN1 from the entomopathogenic fungus Beauveria bassiana is involved in conidiation and virulence. Appl Environ Microbiol 76:254-263
20. Keyhani NO (2012) Using host molecules to increase fungal virulence for biological control of insects. Virulence 3(4):415-417
21. Luo X, Keyhani NO, Yu X, He Z, Luo Z, Pei Y, Zhang Y (2012) The MAP kinase Bbslt2 controls growth, conidiation, cell wall integrity, and virulence in the insect pathogenic fungus Beauveria bassiana. Fungal Genet Biol 49:544-555. doi: 10.1016/j.fgb.2012.05.002
22. Mantilla JG, Galeano NF, Gaitan AL, Cristancho MA, Keyhani NO, Góngora CE (2012) Transcriptome analysis of the entomopathogenic fungus Beauveria bassiana grown on cuticular extracts of the coffee berry borer (Hypothenemus hampei). Microbiology 158:1826-1842. doi: 10.1099/mic.0.051664-0
23. Odds FC, Gow NAR, Brown AJP (2001) Fungal virulence studies come of age. Genome Biol 2(3)
24. Pell JK, Hannam JJ, Steinkraus DC (2010) Conservation biological control using fungal entomopathogens. Biocontrol 55:187-198
25. Retallack DM, Heinecke EL, Gibbons R, Deepe GS, Woods JP (1999) The URA5 gene is necessary for Histoplasma capsulatum growth during infection of mouse and human cells. Infect Immun 67:624-629
26. St Leger R, Joshi L, Bidochka MJ, Roberts DW (1996) Construction of an improved mycoinsecticide overexpressing a toxic protease. Proc Natl Acad Sci USA 93:6349-6354
27. St Leger RJ, Wang CS (2010) Genetic engineering of fungal biocontrol agents to achieve greater efficacy against insect pests. Appl Microbiol Biotechnol 85:901-907
28. Takeno S, Sakuradani E, Murata S, Inohara-Ochiai M, Kawashima H, Ashikari T, Shimizu S (2004) Cloning and sequencing of the ura3 and ura5 genes, and isolation and characterization of uracil auxotrophs of the fungus Mortierella alpina 1S-4. Biosci Biotechnol Biochem 68:277-285
29. Tseng MN, Chung PC, Tzean SS (2011) Enhancing the stress tolerance and virulence of an entomopathogen by metabolic engineering of dihydroxynaphthalene melanin biosynthesis genes. Appl Environ Microbiol 77:4508-4519
30. Wang CS, St Leger RJ (2007) A scorpion neurotoxin increases the potency of a fungal insecticide. Nat Biotechnol 25:1455-1456
31. Xiao G, Ying SH, Zheng P, Wang ZL, Zhang S, Xie XQ, Shang Y, St Leger RJ, Zhao GP, Wang C, Feng MG (2012) Genomic perspectives on the evolution of fungal entomopathogenicity in Beauveria bassiana. Sci Rep 2:483. doi: 10.1038/srep00483
32. Xie XQ, Li F, Ying SH, Feng MG (2012) Additive contributions of two manganese-cored superoxide dismutases (MnSODs) to antioxidation, UV tolerance and virulence of Beauveria bassiana. PLoS One 7(1):e30298. doi: 10.1371/journal.pone.0030298
33. Ying SH, Feng MG (2006) Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana. Appl Microbiol Biotechnol 72:206-210
34. Zhang S, Fan Y, Xia YX, Keyhani NO (2010) Sulfonylurea resistance as a new selectable marker for the entomopathogenic fungus Beauveria bassiana. Appl Microbiol Biotechnol 87:1151-1156. doi: 10.1007/s00253-010-2636-x
35. Zhang S, Widemann E, Bernard G, Lesot A, Pinot F, Pedrini N, Keyhani NO (2012) CYP52X1, representing new cytochrome P450 subfamily, displays fatty acid hydroxylase activity and contributes to virulence and growth on insect cuticular substrates in entomopathogenic fungus Beauveria bassiana. J Biol Chem 287:13477-13486. doi: 10.1074/jbc.M111.338947
36. Zhang SZ, Xia YX, Keyhani NO (2011) Contribution of the gas1 gene of the entomopathogenic fungus Beauveria bassiana, encoding a putative glycosylphosphatidylinositol-anchored β-1,3-glucanosyltransferase, to conidial thermotolerance and virulence. Appl Environ Microbiol 77:2676-2684