Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana

Applied Microbiology and Biotechnology

Volumn 72, Issue 1, 2006, Pages 206-210

  Ying, Sheng Hua ^a   Feng, Ming Guang ^a,b  

^a ZHEJIANG UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BEAUVERIA BASSIANA; PHOSPHINOTHRICIN RESISTANCE; PLASMID VECTORING BAR; PROTEIN GENES;

ABSORPTION; BIODIVERSITY; DNA; FUNGI; GENETIC ENGINEERING; GLYCEROL;

PROTEINS;

ENHANCED GREEN FLUORESCENT PROTEIN; PHOSPHINOTHRICIN; PLASMID VECTOR;

BIOCONTROL AGENT; FLUORESCENCE; FUNGUS; HERBICIDE; PLASMID; PROTEIN; SPORE; TRANSFORMATION;

ABSORPTION; ARTICLE; BAR GENE; BEAUVERIA; BIOLOGICAL CONTROL AGENT; BLASTOSPORE; CELL SUSPENSION; CELL TRANSFORMATION; CULTURE MEDIUM; EGFP GENE; FUNGUS CULTURE; FUNGUS SPORE; GENE; GENE EXPRESSION; GENE TRANSFER; GENETIC MANIPULATION; LOW TEMPERATURE; NONHUMAN;

AMINOBUTYRIC ACIDS; ANTIFUNGAL AGENTS; CORDYCEPS; DRUG RESISTANCE, MULTIPLE, FUNGAL; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HYPOCREALES; PLASMIDS; SPORES, FUNGAL; TRANSFORMATION, GENETIC;

CORDYCEPS BASSIANA;

EID: 33747114763     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0447-x     Document Type: Article

References (26)

1. Andaluz E, Ciudad T, Larriba G (2002) An evaluation of the role of LIG4 in genomic instability and adaptive mutagenesis in Candida albicans. FEMS Yeast Res 2:341-348
2. Barreto CC, Alves LC, Aragao FJ, Rech E, Schrank A, Vainstein MH (1997) High frequency gene transfer by microprojectile bombardment of intact conidia from the entomopathogenic fungus Paecilomyces fumosoroseus. FEMS Microbiol Lett 156:95-99
3. Bidochka MJ, Pfeifer TA, Khachatourians GG (1987) Development of entomopathogenic fungus Beauveria bassiana in liquid culture. Mycopathologia 99:77-83
4. Braga GUL, Flint SD, Miller CD, Anderson AJ, Roberts DW (2001a) Both solar UVA and UVB radiation impair conidial culturability and delay germination in the entomopathogenic fungus, Metarhizium anisopliae. Photochem Photobiol 74:734-739
5. Braga GUL, Flint SD, Miller CD, Anderson AJ, Roberts DW (2001b) Variability in response to UV-B among species and strains of Metarhizium isolated from sites at latitudes from 61 °N to 54 °S. J Invertebr Pathol 78:98-108
6. Brown JS, Aufauvre-Brown A, Holden DW (1998) Insertional mutagenesis of Aspergillus fumigatus. Mol Gen Genet 259:327-335
7. Daboussi MJ, Djeballi A, Gerlinger C, Blaiseau PL, Bouvier I, Cassan M, Lebrun MH, Parisot D, Brygoo Y (1989) Transformation of seven species of filamentous fungi using the nitrate reductase gene of Aspergillus nidulans. Curr Genet 15:453-456
8. de Groot MJA, Bundock P, Hooykaas PJJ, Beijersbergen AGM (1998) Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat Biotechnol 16:839-842
9. dos Reis MC, Fungaro MHP, Duarte RTD, Furlaneto L, Furlaneto MC (2004) Agrobacterium tumefaciens-mediated genetic transformation of the entomopathogenic fungus Beauveria bassiana. J Microbiol Methods 58:197-202
10. Fang WG, Leng B, Xiao YH, Jin K, Ma JC, Fan YH, Feng J, Yang XY, Zhang YJ, Pei Y (2005) Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence. Appl Environ Microbiol 71:363-370
11. Feng MG, Johnson JB, Kish LP (1990) Survey of entomopathogenic fungi naturally infecting cereal aphids in irrigated cereal crops in southwestern Idaho. Environ Entomol 19:1534-1542
12. Feng MG, Poprawski TJ, Khachatourians GG (1994) Production, formulation and application of the entomopathogenic fungus Beauveria bassiana for insect control: current status. Biocontrol Sci Technol 4:3-34
13. Fincham JRS (1989) Transformation in fungi. Microbiol Rev 53:148-170
14. Fungaro MHP, Rech E, Mohlen GS, Vainstein MH, Pascon RC, de Queiroz MV, Pizzirani-Kleiner AA, de Azevedo JL (1995) Transformation of Aspergillus nidulans by microprojectile bombardment on intact conidia. FEMS Microbiol Lett 125:293-298
15. Koukaki M, Giannoutsou E, Karagouni A, Diallinas G (2003) A novel improved method for Aspergillus nidulans transformation. J Microbiol Methods 55:687-695
16. Pfeifer TA, Khachatourians GG (1992) Beauveria bassiana protoplast regeneration and transformation using electroporation. Appl Microbiol Biotechnol 38:376-381
17. Pribylova L, Sychrova H (2003) Efficient transformation of the osmotolerant yeast Zygosaccharomyces rouxii by electroporation. J Microbiol Methods 55:481-484
18. Rangel DEN, Braga GUL, Anderson AJ, Roberts DW (2005) Variability in conidial thermotolerance of Metarhizium anisopliae, isolates from different geographic origins. J Invertebr Pathol 88:116-125
19. Reader U, Broda P (1985) Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol 1:17-20
20. Roberts DW, St. Leger RJ (2004) Metarhizium spp., cosmopolitan insect-pathogenic fungi: mycological aspects. Adv Appl Microbiol 54:1-70
21. Sandhu SS, Unkles SE, Rajak RC, Kinghorn JR (2001) Generation of benomyl resistant Beauveria bassiana strains and their infectivity against Helicoverpa armigera. Biocontrol Sci Technol 11:250-254
22. Schiestl RH, Gietz RD (1989) High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr Genet 16:339-346
23. Wraight SP, Jackson MA, de Kock SL (2001) Production, stabilization and formulation of fungal biological agents. In: Butt TM, Jackson C, Magan N (eds) Fungal biocontrol agents-progress, problems and potential. CAB International, Wallingford, pp 253-287
24. Ye SD, Dun YH, Feng MG (2005) Time and concentration dependent interactions of Beauveria bassiana with sublethal rates of imidacloprid against the aphid pests Macrosiphoniella sanborni and Myzus persicae. Ann Appl Biol 146:459-468
25. Ying SH, Feng MG (2004) Relationship between thermotolerance and hydrophobin-like proteins in aerial conidia of Beauveria bassiana and Paecilomyces fumosoroseus as fungal biocontrol agents. J Appl Microbiol 97:323-331
26. Ypsilos IK, Magan N (2005) Characterisation of optimum cultural environmental conditions for the production of high numbers of Metarhizium anisopliae blastospores with enhanced ecological fitness. Biocontrol Sci Technol 15:683-699