Lactobacillus species isolated from vaginal secretions of healthy and bacterial vaginosis-intermediate Mexican women: A prospective study

BMC Infectious Diseases

Volumn 13, Issue 1, 2013, Pages

  Martínez Peña, Marcos Daniel ^a,b   Castro Escarpulli, Graciela ^a   Aguilera Arreola, Ma Guadalupe ^a  

^a INSTITUTO POLITÉCNICO NACIONAL   (Mexico)

^b INSTITUTO NACIONAL DE INVESTIGACIONES FORESTALES   (Mexico)

Author keywords

16S rRNA; Bacterial vaginosis; Lactobacilli; Mexican population; Species identification; Vaginal microbiota

Indexed keywords

RIBOSOME RNA; RNA 16S;

ADULT; AGED; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM ISOLATION; CONTROLLED STUDY; ENTEROCOCCUS; ETHNIC GROUP; FEMALE; HUMAN; LACTOBACILLUS; LACTOBACILLUS ACIDOPHILUS; LACTOBACILLUS BREVIS; LACTOBACILLUS BUCHNERI; LACTOBACILLUS CASEI; LACTOBACILLUS CRISPATUS; LACTOBACILLUS FERMENTUM; LACTOBACILLUS GASSERI; LACTOBACILLUS JENSENII; LACTOBACILLUS REUTERI; LACTOBACILLUS RHAMNOSUS; MEXICAN; MOLECULAR MODEL; NUCLEOTIDE SEQUENCE; NUGENT SCORE; PROSPECTIVE STUDY; RNA GENE; RNA SEQUENCE; SCORING SYSTEM; VAGINA DISCHARGE; VAGINAL SECRETION; VAGINITIS; WOMEN'S HEALTH;

ADULT; DNA, BACTERIAL; FEMALE; HUMANS; LACTOBACILLUS; MEXICO; PHYLOGENY; PROSPECTIVE STUDIES; RNA, RIBOSOMAL, 16S; VAGINA; VAGINOSIS, BACTERIAL;

EID: 84876665476     PISSN: None     EISSN: 14712334     Source Type: Journal    
DOI: 10.1186/1471-2334-13-189     Document Type: Article

References (42)

1. Ma B, Forney LJ, Ravel J. Vaginal microbiome: rethinking health and disease. Annu Rev Microbiol 2012, 66:371-389. 10.1146/annurev-micro-092611-150157, 22746335.
2. Angeles-López M, García-Cano E, Aquino SC. Hydrogen peroxide production and resistance to nonoxinol-9 in Lactobacillus spp. isolated from the vagina of reproductive age women. Rev Latinoam Microbiol 2001, 43:171-176.
3. Antonio MA, Hawes SE, Hillier SL. The identification of vaginal Lactobacillus species and the demographic and microbiologic characteristics of women colonized by these species. J Infect Dis 1999, 180:1950-1956. 10.1086/315109, 10558952.
4. Turovskiy Y, Sutyak Noll K, Chikindas ML. The aetiology of bacterial vaginosis. J Appl Microbiol 2011, 110:1105-1128. 10.1111/j.1365-2672.2011.04977.x, 3072448, 21332897.
5. Gillet E, Meys JF, Verstraelen H, Bosire C, De Sutter P, Temmerman M, Broeck DV. Bacterial vaginosis is associated with uterine cervical human papillomavirus infection: a meta-analysis. BMC Infect Dis 2011, 11:10. [Published online], 10.1186/1471-2334-11-10, 3023697, 21223574.
6. Zhou X, Brown CJ, Abdo Z, Davis CC, Hansmann MA, Joyce P, Foster JA, Forney LJ. Differences in the composition of vaginal microbial communities found in healthy Caucasian and black women. ISME J 2007, 1:121-133. 10.1038/ismej.2007.12, 18043622.
7. Vásquez A, Jakobsson T, Ahrné S, Forsum U, Molin G. Vaginal Lactobacillus flora of healthy Swedish women. J Clin Microbiol 2002, 40:2746-2749. 10.1128/JCM.40.8.2746-2749.2002, 120688, 12149323.
8. Pérez-Miravete A. Studies on the vaginal flora IX Classification of Lactobacilli of vaginal origin. Rev Latinoam Microbiol Parasitol 1967, 9:11-14.
9. Hernández-Rodríguez C, Romero-González R, Albani-Campanario M, Figueroa-Damián R, Meraz-Cruz N, Hernández-Guerrero C. Vaginal microbiota of healthy pregnant Mexican women is constituted by four Lactobacillus species and several vaginosis-associated bacteria. Infect Dis Obstet Gynecol 2011, 2011:851485. 3179877, 21960733.
10. Nigatu A. Evaluation of numerical analyses of RAPD and API 50 CH patterns to differentiate Lactobacillus plantarum, L. fermentum, L. rhamnosus, L. sake, L. parabuchneri, L. gallinarum, L. casei, Weissella minor and related taxa isolated from kocho and tef. J Appl Microbiol 2000, 89:969-978. 10.1046/j.1365-2672.2000.01202.x, 11123470.
11. Boyd MA, Antonio MA, Hillier SL. Comparison of API 50 CH strips to whole-chromosomal DNA probes for identification of Lactobacillus species. J Clin Microbiol 2005, 43:5309-5311. 10.1128/JCM.43.10.5309-5311.2005, 1248469, 16208005.
12. Srinivasan S, Fredricks DN. The human vaginal bacterial biota and bacterial vaginosis. Interdiscip Perspect Infect Dis 2008, 2008:750479. 2648628, 19282975.
13. Verhelst R, Verstraelen H, Claeys G, Verschraegen G, Van Simaey L, De Ganck C, De Backer E, Temmerman M, Vaneechoutte M. Comparison between Gram stain and culture for the characterization of vaginal microflora: definition of a distinct grade that resembles grade I microflora and revised categorization of grade I microflora. BMC Microbiol 2005, 14(5):61.
14. Santiago GL, Cools P, Verstraelen H, Trog M, Missine G, El Aila N, Verhelst R, Tency I, Claeys G, Temmerman M, Vaneechoutte M. Longitudinal study of the dynamics of vaginal microflora during two consecutive menstrual cycles. PLoS One 2011, 6(11):e28180. 10.1371/journal.pone.0028180, 3227645, 22140538.
15. Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Ng J, Munro K, Alatossava T. Identification of Lactobacillus isolates from the gastrointestinal tract, silage, and yoghurt by 16S-23S rRNA gene intergenic spacer region sequence comparisons. Appl Environ Microbiol 1999, 65:4264-4267. 99775, 10473450.
16. O'Sullivan DJ. Methods for analysis of the intestinal microflora. Curr Issues Intest Microbiol 2000, 1:39-50.
17. Ravel J, Gajer P, Abdo Z, Schneider GM, Koenig SS, McCulle SL, Karlebach S, Gorle R, Russell J, Tacket CO, Brotman RM, Davis CC, Ault K, Peralta L, Forney LJ. Vaginal microbiome of reproductive-age women. Proc Natl Acad Sci USA 2011, 108:4680-4687. 10.1073/pnas.1002611107, 3063603, 20534435.
18. Verstraelen H, Verhelst R, Claeys G, De Backer E, Temmerman M, Vaneechoutte M. Longitudinal analysis of the vaginal microflora in pregnancy suggests that L. crispatus promotes the stability of the normal vaginal microflora and that L. gasseri and/or L. iners are more conducive to the occurrence of abnormal vaginal microflora. BMC Microbiol 2009, 9:116. 10.1186/1471-2180-9-116, 2698831, 19490622.
19. Hernández-Martínez F, Hernández-García JA, Martínez-Peña MD, Muñiz-Becerril BL, Hernández-Cortez C, Castro-Escarpulli G, Aguilera-Arreola MG. Aetiology and frecuency of cervico-vaginal infections among Mexican women. AJMR 2013, 7:27-34.
20. Nugent RP, Krohn MA, Hillier SL. Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation. J Clin Microbiol 1991, 29:297-301. 269757, 1706728.
21. Vila J, Marcos MA, Jimenez de Anta MT. A comparative study of different PCR-based DNA fingerprinting techniques for typing of the Acinetobacter calcoaceticus-A. baumannii complex. J Med Microbiol 1996, 44:482-489. 10.1099/00222615-44-6-482, 8636966.
22. Borrell N, Acinas SG, Figueras MJ, Martínez-Murcia AJ. Identification of Aeromonas clinical isolates by restriction fragment length polymorphism of PCR-amplified 16S rRNA genes. J Clin Microbiol 1997, 45:1671-1674.
23. Gouy M, Guindon S, Gascuel O. SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol 2010, 27:221-224. 10.1093/molbev/msp259, 19854763.
24. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, López R, Thompson JD, Gibson FJ, Higgins DG. Clustal W and clustal X version 2 0. Bioinformatics 2007, 23:2947-2948. 10.1093/bioinformatics/btm404, 17846036.
25. Galtier N, Gouy M, Gautier C. SEAVIEW and PHYLO_WIN: two graphic tools for sequence alignment and molecular phylogeny. Comput Applic Biosci 1996, 12:543-548.
26. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4 0. Mol Biol Evol 2007, 24:1596-1599. 10.1093/molbev/msm092, 17488738.
27. Rosselló-Mora R, Amann R. The species concept for prokaryotes. FEMS Microbiol Rev 2001, 25:39-67.
28. Morgulis A, Coulouris G, Raytselis Y, Madden TL, Agarwala R, Schäffer AA. Database indexing for production megablast searches. Bioinformatics 2008, 24:1757-1764. 10.1093/bioinformatics/btn322, 2696921, 18567917.
29. Ljungh A, Wadström T. Lactobacillus Molecular Biology: From Genomics to Probiotics 2009, Norfolk, UK: Caister Academic Press.
30. Antonio MA, Rabe LK, Hillier SL. Colonization of the rectum by Lactobacillus species and decreased risk of bacterial vaginosis. J Infect Dis 2005, 192:394-398. 10.1086/430926, 15995952.
31. Slover CM, Danzinger L. Lactobacillus: a review. Clin Microbiol Newsl 2008, 30:23-27.
32. Pavlova SI, Kilic AO, Kilic SS, So JS, Nader-Macias ME, Simoes JA, Tao L. Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences. J Appl Microbiol 2002, 92:451-459. 10.1046/j.1365-2672.2002.01547.x, 11872120.
33. Falsen E, Pascual C, Sjödén B, Ohlén M, Collins MD. Phenotypic and phylogenetic characterization of a novel Lactobacillus species from human sources: description of Lactobacillus iners sp nov. Int J Syst Bacteriol 1999, 49:217-221. 10.1099/00207713-49-1-217, 10028266.
34. Petricevic L, Domig KJ, Nierscher FJ, Krondorfer I, Janitschek C, Kneifel W, Kiss H. Characterisation of the oral, vaginal and rectal Lactobacillus flora in healthy pregnant and postmenopausal women. Eur J Obstet Gynecol Reprod Biol 2012, 160:93-99. 10.1016/j.ejogrb.2011.10.002, 22088236.
35. Dimitonova SP, Bakalov BV, Aleksandrova-Georgieva RN, Danova ST. Phenotypic and molecular identification of lactobacilli isolated from vaginal secretions. J Microbiol Immunol Infect 2008, 41:469-477.
36. Anukam KC, Osazuwa EO, Ahonkhai I, Reid G. Lactobacillus vaginal microbiota of women attending a reproductive health care service in Benin city, Nigeria. Sex Transm Dis 2006, 33:59-62. 10.1097/01.olq.0000175367.15559.c4, 16385223.
37. Jespers V, Menten J, Smet H, Poradosú S, Abdellati S, Verhelst R, Hardy L, Buvé A, Crucitti T. Quantification of bacterial species of the vaginal microbiome in different groups of women, using nucleic acid amplification tests. BMC Microbiol 2012, 12:83. 10.1186/1471-2180-12-83, 3418157, 22647069.
38. Zhou X, Hansmann MA, Davis CC, Suzuki H, Brown CJ, Schütte U, Pierson JD, Forney LJ. The vaginal bacterial communities of Japanese women resemble those of women in other racial groups. FEMS Immunol Med Microbiol 2010, 58:169-181. 10.1111/j.1574-695X.2009.00618.x, 2868947, 19912342.
39. De Backer E, Verhelst R, Verstraelen H, Alqumber MA, Burton JP, Tagg JR, Temmerman M, Vaneechoutte M. Quantitative determination by real-time PCR of four vaginal Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. iners. BMC Microbiol 2007, 7:115. 10.1186/1471-2180-7-115, 2233628, 18093311.
40. Fredricks DN, Fiedler TL, Marrazzo JM. Molecular identification of bacteria associated with bacterial vaginosis. N Engl J Med 2005, 353:1899-1911. 10.1056/NEJMoa043802, 16267321.
41. Stephenson DP, Moore RJ, Allison GE. Comparison and utilization of repetitive-blement PCR techniques for typing Lactobacillus isolates from the chicken gastrointestinal tract. Appl Environ Microbiol 2009, 75:6764-6776. 10.1128/AEM.01150-09, 2772419, 19749057.
42. Spear GT, Gilbert D, Landay AL, Zariffard R, French AL, Patel P, Gillevet PM. Pyrosequencing of the genital microbiotas of HIV-seropositive and -seronegative women reveals Lactobacillus iners as the predominant Lactobacillus species. Appl Environ Microbiol 2011, 77:378-381. 10.1128/AEM.00973-10, 3019699, 21075899.