New insight into the molecular switch mechanism of human Rho family proteins: Shifting a paradigm

Biological Chemistry

Volumn 394, Issue 1, 2013, Pages 89-95

  Ahmadian, Mohammad Reza ^a   Jaiswal, Mamta ^a   Fansa, Eyad Kalawy ^a   Dvorsky, Radovan ^a  

^a Department of Orthopedic Surgery   (Germany)

Author keywords

GTP hydrolysis; Nucleotide association; Nucleotide dissociation; Rho family; RhoD; Rif

Indexed keywords

PROTEIN RAC1B; PROTEIN RAC3; PROTEIN RHOB; PROTEIN RHOC; PROTEIN RHOD; PROTEIN RHOG; PROTEIN RHOH; PROTEIN RIF; PROTEIN RND1; PROTEIN RND2; PROTEIN RND3; PROTEIN TC10; PROTEIN TCL; PROTEIN VARIANT; RAC1 PROTEIN; RAC2 PROTEIN; RHO FACTOR; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; HUMAN; HYDROLYSIS; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN STRUCTURE; QUANTITATIVE ANALYSIS;

GUANOSINE TRIPHOSPHATE; HUMANS; MODELS, MOLECULAR; RHO GTP-BINDING PROTEINS;

EID: 84876590830     PISSN: 14316730     EISSN: 14374315     Source Type: Journal    
DOI: 10.1515/hsz-2012-0207     Document Type: Article

References (40)

1. Bishop, A.L. and Hall, A. (2000). Rho GTPases and their effector proteins. Biochem. J. 348 Pt 2, 241-255. (Pubitemid 30345195)
2. Boureux, A., Vignal, E., Faure, S., and Fort, P. (2007) Evolution of the Rho family of ras-like GTPases in eukaryotes. Mol. Biol. Evol. 24, 203-216. (Pubitemid 46026295)
3. Bourne, H.R., Sanders, D.A., and McCormick, F. (1991). The GTPase superfamily: conserved structure and molecular mechanism. Nature 349, 117-127. (Pubitemid 21912023)
4. Burridge, K. and Wennerberg, K. (2004). Rho and Rac take center stage. Cell 116, 167-179. (Pubitemid 38167310)
5. Dovas, A. and Couchman, J.R. (2005). RhoGDI: multiple functions in the regulation of Rho family GTPase activities. Biochem. J. 390, 1-9. (Pubitemid 41192225)
6. Dvorsky, R. and Ahmadian, M.R. (2004). Always look on the bright site of Rho: structural implications for a conserved intermolecular interface. EMBO Rep. 5, 1130-1136. (Pubitemid 40103605)
7. Eberth, A. and Ahmadian, M.R. (2009). In vitro GEF and GAP assays. Curr. Protoc. Cell Biol. Chapter 14, Unit 14.9.
8. Eberth, A., Dvorsky, R., Becker, C.F., Beste, A., Goody, R.S., and Ahmadian, M.R. (2005). Monitoring the real-time kinetics of the hydrolysis reaction of guanine nucleotide-binding proteins. Biol. Chem. 386, 1105-1114. (Pubitemid 41705365)
9. Etienne-Manneville, S. and Hall, A. (2002). Rho GTPases in cell biology. Nature 420, 629-635. (Pubitemid 36764489)
10. Fiegen, D., Blumenstein, L., Stege, P., Vetter, I.R., and Ahmadian, M.R. (2002). Crystal structure of Rnd3/RhoE: functional implications. FEBS Lett. 525, 100-104.
11. Fiegen, D., Haeusler, L.C., Blumenstein, L., Herbrand, U., Dvorsky, R., Vetter, I.R., and Ahmadian, M.R. (2004). Alternative splicing of Rac1 generates Rac1b, a self-activating GTPase. J. Biol. Chem. 279, 4743-4749. (Pubitemid 38199069)
12. Garavini, H., Riento, K., Phelan, J.P., McAlister, M.S., Ridley, A.J., and Keep, N.H. (2002). Crystal structure of the core domain of RhoE/Rnd3: a constitutively activated small G protein. Biochemistry 41, 6303-6310. (Pubitemid 34525994)
13. Gu, Y., Zheng, Y., and Williams, D.A. (2005). RhoH GTPase: a key regulator of hematopoietic cell proliferation and apoptosis ? Cell Cycle 4, 201-202. (Pubitemid 41365314)
14. Haeusler, L.C., Blumenstein, L., Stege, P., Dvorsky, R., and Ahmadian, M.R. (2003). Comparative functional analysis of the Rac GTPases. FEBS Lett. 555, 556-560. (Pubitemid 37532610)
15. Haeusler, L.C., Hemsath, L., Fiegen, D., Blumenstein, L., Herbrand, U., Stege, P., Dvorsky, R., and Ahmadian, M.R. (2006). Purification and biochemical properties of Rac1, 2, 3 and the splice variant Rac1b. Methods Enzymol. 406, 1-11. (Pubitemid 43207427)
16. Heasman, S.J. and Ridley, A.J. (2008). Mammalian Rho GTPases: new insights into their functions from in vivo studies. Nature Rev. Mol. Cell Biol. 9, 690-701.
17. Hemsath, L. and Ahmadian, M.R. (2005). Fluorescence approaches for monitoring interactions of Rho GTPases with nucleotides, regulators, and effectors. Methods 37, 173-182. (Pubitemid 41587426)
18. Ihara, K., Muraguchi, S., Kato, M., Shimizu, T., Shirakawa, M., Kuroda, S., Kaibuchi, K., and Hakoshima, T. (1998). Crystal structure of human RhoA in a dominantly active form complexed with a GTP analogue. J. Biol. Chem. 273, 9656-9666. (Pubitemid 28183055)
19. Jaiswal, M., Gremer, L., Dvorsky, R., Haeusler, L.C., Cirstea, I.C., Uhlenbrock, K., and Ahmadian, M.R. (2011). Mechanistic insights into specificity, activity, and regulatory elements of the regulator of G-protein signaling (RGS)-containing Rho-specific guanine nucleotide exchange factors (GEFs) p115, PDZ-RhoGEF (PRG), and leukemia-associated RhoGEF (LARG). J. Biol. Chem. 286, 18202-18212.
20. Jaiswal, M., Dubey, B.N., Koessmeier, K.T., Gremer, L., and Ahmadian, M.R. (2012). Biochemical assays to characterize Rho GTPases. Methods Mol. Biol. 827, 37-58.
21. John, J., Sohmen, R., Feuerstein, J., Linke, R., Wittinghofer, A., and Goody, R.S (1990). Kinetics of Interaction of Nucleotides with Nucleotide-free H-ras p21. Biochemistry 29, 6058-6065. (Pubitemid 20201412)
22. Joneson, T., White, M.A., Wigler, M.H., and Bar-Sagi, D. (1996). Stimulation of membrane ruffling and MAP kinase activation by distinct effectors of RAS. Science 271, 810-812. (Pubitemid 26060736)
23. Kabcenell, A.K., Goud, B., Northup, J.K., and Novick, P.J. (1990). Binding and hydrolysis of guanine nucleotides by Sec4p, a yeast protein involved in the regulation of vesicular traffic. J. Biol. Chem. 265, 9366-9372.
24. Klebe, C., Prinz, H., Wittinghofer, A., and Goody, R.S. (1995). The kinetic mechanism of Ran-nucleotide exchange catalyzed by RCC1.Biochemistry 34, 12543-12552.
25. Li, G. and Zhang, X.C. (2004). GTP hydrolysis mechanism of Ras-like GTPases. J. Mol. Biol. 340, 921-932. (Pubitemid 38968691)
26. Li, X., Bu, X., Lu, B., Avraham, H., Flavell, R.A., and Lim, B. (2002). The hematopoiesis-specific GTP-binding protein RhoH is GTPase deficient and modulates activities of other Rho GTPases by an inhibitory function. Mol. Cell. Biol. 22, 1158-1171. (Pubitemid 34094852)
27. Michaelson, D., Silletti, J., Murphy, G., D ' Eustachio, P., Rush, M., and Philips, M.R. (2001). Differential localization of Rho GTPases in live cells: regulation by hypervariable regions and RhoGDI binding. J. Cell Biol. 152, 111-126. (Pubitemid 32102441)
28. Randazzo, P.A., Terui, T., Sturch, S., Fales, H.M., Ferrige, A.G., and Kahn, R.A. (1995). The myristoylated amino terminus of ADP-ribosylation factor 1 is a phospholipid-and GTP-sensitive switch. J. Biol. Chem. 270, 14809-14815.
29. Riou, P., Villalonga, P., and Ridley, A.J. (2010). Rnd proteins: multifunctional regulators of the cytoskeleton and cell cycle progression. Bioessays 32, 986-992.
30. Rittinger, K., Walker, P.A., Eccleston, J.F., Smerdon, S.J., and Gamblin, S.J. (1997). Structure at 1.65 A of RhoA and its GTPase-activating protein in complex with a transition-state analogue. Nature 389, 758-762. (Pubitemid 27458966)
31. Rossman, K.L., Worthylake, D.K., Snyder, J.T., Cheng, L., Whitehead, I.P., and Sondek, J. (2002). Functional analysis of cdc42 residues required for guanine nucleotide exchange. J. Biol. Chem. 277, 50893-50898. (Pubitemid 36042256)
32. Scheffzek, K., Ahmadian, M.R., and Wittinghofer, A. (1998). GTPaseactivating proteins: helping hands to complement an active site. Trends Biochem. Sci. 23, 257-262. (Pubitemid 28343370)
33. Shutes, A., Berzat, A.C., Chenette, E.J., Cox, A.D., and Der, C.J. (2006). Biochemical analyses of the Wrch atypical Rho family GTPases. Methods Enzymol. 406, 11-26. (Pubitemid 43207428)
34. Simon, I., Zerial, M., and Goody, R.S. (1996). Kinetics of interaction of Rab5 and Rab7 with nucleotides and magnesium ions. J. Biol. Chem. 271, 20470-20478. (Pubitemid 26281819)
35. Spoerner, M., Herrmann, C., Vetter, I.R., Kalbitzer, H.R., and Wittinghofer, A. (2001). Dynamic properties of the Ras switch I region and its importance for binding to effectors. Proc. Natl. Acad. Sci. USA 98, 4944-4949. (Pubitemid 32397062)
36. Wei, Y., Zhang, Y., Derewenda, U., Liu, X., Minor, W., Nakamoto, R.K., Somlyo, A.V., Somlyo, A.P., and Derewenda, Z.S. (1997). Crystal structure of RhoA-GDP and its functional implications. Nat. Struct. Biol. 4, 699-703.
37. Wennerberg, K. and Der, C.J. (2004). Rho-family GTPases: it ' s not only Rac and Rho (and I like it). J. Cell Sci. 117, 1301-1312. (Pubitemid 38559808)
38. White, M.A., Nicolette, C., Minden, A., Polverino, A., Van Aelst, L., Karin, M., and Wigler, M.H. (1995). Multiple Ras functions can contribute to mammalian cell transformation. Cell 80, 533-541.
39. Wittinghofer, A. and Vetter, I.R. (2011). Structure-function relationships of the G domain, a canonical switch motif. Annu. Rev. Biochem. 80, 943-971.
40. Zhang, B., Zhang, Y., Wang, Z., and Zheng, Y. (2000). The role of Mg 2 + cofactor in the guanine nucleotide exchange and GTP hydrolysis reactions of Rho family GTP-binding proteins. J. Biol. Chem. 275, 25299-25307.