Polymerase chain reaction-based screening for the ceftriaxone-resistant Neisseria gonorrhoeae F89 strain

Eurosurveillance

Volumn 18, Issue 14, 2013, Pages

  Goire, N ^a,b   Lahra, M M ^c   Ohnishi, M ^d   Hogan, T ^c   Liminios, A E ^c   Nissen, M D ^a,b,e   Sloots, T P ^a,b,e   Whiley, D M ^a,b  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b Sir Albert Sakzewski Virus Research Centre   (Australia)

^c PRINCE OF WALES HOSPITAL   (Australia)

^d NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^e Central Queensland Pathology Laboratory   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CEFTRIAXONE; PENICILLIN BINDING PROTEIN;

ANTIBIOTIC RESISTANCE; ARTICLE; DISEASE SURVEILLANCE; HIGH RESOLUTION MELTING ANALYSIS; LIMIT OF QUANTITATION; MINIMUM INHIBITORY CONCENTRATION; NEISSERIA GONORRHOEAE; NONHUMAN; NUCLEIC ACID AMPLIFICATION; NUCLEOTIDE SEQUENCE; PREVALENCE; REAL TIME POLYMERASE CHAIN REACTION;

EID: 84876502232     PISSN: 1025496X     EISSN: 15607917     Source Type: Journal    
DOI: 10.2807/1560-7917.ES2013.18.14.20444     Document Type: Article

References (13)

1. Ohnishi M, Golparian D, Shimuta K, Saika T, Hoshina S, Iwasaku K, et al. Is Neisseria gonorrhoeae initiating a future era of untreatable gonorrhea?: detailed characterization of the first strain with high-level resistance to ceftriaxone. Antimicrob Agents Chemother. 2011;55(7): 3538-45. http://dx.doi.org/10.1128/AAC.00325-11 PMid:21576437 PMCid:3122416
2. Unemo M, Golparian D, Nicholas R, Ohnishi M, Gallay A, Sednaoui P. High-level cefixime-and ceftriaxone-resistant N. gonorrhoeae in France: novel penA mosaic allele in a successful international clone causes treatment failure. Antimicrob Agents Chemother. 2012;56(3):1273-80. http://dx.doi.org/10.1128/AAC.05760-11 PMid:22155830 PMCid:3294892
3. Cámara J, Serra J, Ayats J, Bastida T, Carnicer-Pont D, Andreu A, et al. Molecular characterization of two high-level ceftriaxoneresistant Neisseria gonorrhoeae isolates detected in Catalonia, Spain. J Antimicrob Chemother. 2012;67(8):1858-60. http://dx.doi.org/10.1093/jac/dks162 PMid:22566592
4. Takahata S, Senju N, Osaki Y, Yoshida T, Ida T. Amino acid substitutions in mosaic penicillin-binding protein 2 associated with reduced susceptibility to cefixime in clinical isolates of Neisseria gonorrhoeae. Antimicrob Agents Chemother. 2006;50(11):3638-45. http://dx.doi.org/10.1128/AAC.00626-06 PMid:16940068 PMCid:1635225
5. Whiley DM, Limnios EA, Ray S, Sloots TP, Tapsall JW. Diversity of penA alterations and subtypes in Neisseria gonorrhoeae strains from Sydney, Australia, that are less susceptible to ceftriaxone. Antimicrob Agents Chemother. 2007;51(9):3111-16. http://dx.doi.org/10.1128/AAC.00306-07 PMid:17591846 PMCid:2043202
6. Tomberg J, Unemo M, Davies C, Nicholas RA. Molecular and structural analysis of mosaic variants of penicillin-binding protein 2 conferring decreased susceptibility to expandedspectrum cephalosporins in Neisseria gonorrhoeae: role of epistatic mutations. Biochemistry. 2010;49(37):8062-70. http://dx.doi.org/10.1021/bi101167x PMid:20704258 PMCid:2939205
7. Ohnishi M, Watanabe Y, Ono E, Takahashi C, Oya H, Kuroki T, et al. Spread of a chromosomal cefixime-resistant penA gene among different Neisseria gonorrhoeae lineages. Antimicrob Agents Chemother. 2010;54(3):1060-7. http://dx.doi. org/10.1128/AAC.01010-09 PMid:20028823 PMCid:2826007
8. Goire N, Ohnishi M, Limnios AE, Lahra MM, Lambert SB, Nimmo GR, et al. Enhanced gonococcal antimicrobial surveillance in the era of ceftriaxone resistance: a real-time PCR assay for direct detection of the Neisseria gonorrhoeae H041 strain. J Antimicrob Chemother. 2011;67(4):902-5. http://dx.doi. org/10.1093/jac/dkr549 PMid:22207596
9. Goire N, Freeman K, Lambert SB, Nimmo GR, Limnios AE, Lahra MM, et al. The influence of target population on nonculturebased detection of markers of Neisseria gonorrhoeae antimicrobial resistance. Sex Health. 2012;9(5):422-9. http://dx.doi.org/10.1071/SH12026 PMid:23036167
10. Ochiai S, Ishiko H, Yasuda M, Deguchi T. Rapid detection of the mosaic structure of the Neisseria gonorrhoeae penA Gene, which is associated with decreased susceptibilities to oral cephalosporins. J Clin Microbiol. 2008;46(5):1804-10. http://dx.doi.org/10.1128/JCM.01800-07 PMid:18367575 PMCid:2395096
11. Whiley DM, Jacob K, Nakos J, Bletchly C, Nimmo GR, Nissen MD, et al. Improved detection of genetic markers of antimicrobial resistance by hybridization probe-based melting curve analysis using primers to mask proximal mutations: examples include the influenza H275Y substitution. J Antimicrob Chemother. 2012;67(6):1375-9. http://dx.doi.org/10.1093/jac/dks040 PMid:22334604
12. Goire N, Nissen MD, LeCornec GM, Sloots TP, Whiley DM. A duplex Neisseria gonorrhoeae real-time polymerase chain reaction assay targeting the gonococcal porA pseudogene and multicopy opa genes. Diagn Microbiol Infect Dis. 2008;61(1):6-12. http://dx.doi.org/10.1016/j.diagmicrobio.2007.12.007 PMid:18248938
13. Tapsall JW. Neisseria gonorrhoeae and emerging resistance to extended spectrum cephalosporins. Curr Opin Infect Dis. 2009;22(1):87-91. http://dx.doi.org/10.1097/ QCO.0b013e328320a836 PMid:19532086