Improved detection of genetic markers of antimicrobial resistance by hybridization probe-based melting curve analysis using primers to mask proximal mutations: Examples include the influenza H275Y substitution

Journal of Antimicrobial Chemotherapy

Volumn 67, Issue 6, 2012, Pages 1375-1379

  Whiley, David M ^a,b   Jacob, Kevin ^a,b   Nakos, Jennifer ^c   Bletchly, Cheryl ^c   Nimmo, Graeme R ^c,d   Nissen, Michael D ^a,b,c   Sloots, Theo P ^a,b,c  

^a Queensland Children's Medical Research Institute   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

^c Central Queensland Pathology Laboratory   (Australia)

^d GRIFFITH UNIVERSITY   (Australia)

Author keywords

Influenza; PCR; Variation

Indexed keywords

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL STRAIN; DNA SEQUENCE; GENE MUTATION; GENETIC MARKER; HYBRIDIZATION PROBE BASED MELTING CURVE ANALYSIS; INFLUENZA VIRUS A; INFLUENZA VIRUS A H1N1; INFLUENZA VIRUS A H275Y; MELTING POINT; NEISSERIA GONORRHOEAE; NONHUMAN; NUCLEIC ACID ANALYSIS; NUCLEIC ACID BASE SUBSTITUTION; SINGLE NUCLEOTIDE POLYMORPHISM; VIRUS DETECTION; VIRUS STRAIN;

DRUG RESISTANCE, BACTERIAL; DRUG RESISTANCE, VIRAL; HUMANS; INFLUENZA A VIRUS, H1N1 SUBTYPE; MICROBIAL SENSITIVITY TESTS; MOLECULAR DIAGNOSTIC TECHNIQUES; NEISSERIA GONORRHOEAE; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE PROBES; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; TRANSITION TEMPERATURE;

EID: 84861108425     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dks040     Document Type: Article

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