Isolation and characterization of a novel oxidant- and surfactant-stable extracellular alkaline protease from e xiguobacterium profundum BK-P23

Biotechnology and Applied Biochemistry

Volumn 60, Issue 2, 2013, Pages 155-161

  Anbu, Periasamy ^a   Hur, Byung Ki ^a   Gyun Lee, Choul ^a  

^a Inha University   (South Korea)

Author keywords

alkaline protease; Exiguobacterium profundum; properties of enzymes; purification

Indexed keywords

ALKALINE PROTEASE; AMMONIUM SULFATE PRECIPITATION; DETERGENT INDUSTRY; EXIGUOBACTERIUM PROFUNDUM; ISOLATION AND CHARACTERIZATION; NONAQUEOUS MEDIA; PEPTIDE SYNTHESIS; PHENYLMETHYLSULFONYL FLUORIDE;

AMINO ACIDS; ENZYME ACTIVITY; ORGANIC SOLVENTS; OXIDATION; PURIFICATION; SURFACE ACTIVE AGENTS;

ALKALINITY;

ALKALINE PROTEINASE; AMMONIUM SULFATE; BACTERIAL ENZYME; BENZYLSULFONYL FLUORIDE; ORGANIC SOLVENT; OXIDIZING AGENT; POLYSORBATE 80; PROTEINASE INHIBITOR; RNA 16S; SERINE PROTEINASE; SURFACTANT; TRITON X 100;

ARTICLE; COLUMN CHROMATOGRAPHY; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME STABILITY; EXIGUOBACTERIUM PROFUNDUM; GENE SEQUENCE; GRAM POSITIVE BACTERIUM; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION;

BACILLUS; BACTERIAL PROTEINS; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; ENZYME STABILITY; HOT TEMPERATURE; HYDROGEN-ION CONCENTRATION; OXIDANTS; SURFACE-ACTIVE AGENTS;

EXIGUOBACTERIUM;

EID: 84876461733     PISSN: 08854513     EISSN: 14708744     Source Type: Journal    
DOI: 10.1002/bab.1059     Document Type: Article

References (36)

1. Miyaji, T., Otta, Y., Shibata, T., Mitsui, K., Nakagawa, T., Watanabe, T., Niimura, Y., and, Tomizuka, N., (2005) Lett. Appl. Microbiol. 41, 253-257.
2. Ng, T. K., and, Kenealy, W. F., (1986) In Thermophiles. General Molecular and applied microbiology (, Brock T. D., ed.). pp. 197-205, John Wiley, New York.
3. Amoozegara, M. A., Fatemia, A. Z., Ksrbalaei-Heidarib, H. R., and, Razavic, M. R. R., (2007) Microbiol. Res. 162, 369-377.
4. Phadatare, S. U., Deshpande, V. V., and, Srinivasan, M. C., (1993) Enzyme Microb. Technol. 15, 72-76.
5. Godfrey, T., and, West, S,. (1996) Industrial Enzymology. pp. 3-10, 4th ed., Macmillan, New York.
6. Bayoudh, A., Gharsallah, N., Chamkha, M., Dhouib, A., Ammar, S., and, Nasri, M., (2000) J. Ind. Microbiol. Biotechnol. 24, 291-295.
7. Kazan, D., Denizci, A. A., Kerimak Oner, M. N., and, Erarslan, A., (2005) J. Ind. Microbiol. Biotechnol. 32, 335-344.
8. Tremacoldi, C. R., Monti, R., Selistre-De-Araujo, H. S., and, Carmona, E. C., (2007) World J. Microbiol. Biotechnol. 23, 295-299.
9. Kumar, C. G., and, Takagi, H., (1999) Biotechnol. Adv. 17, 561-594.
10. Anwar, A., and, Saleemuddin, M., (2000) Biotechnol. Appl. Biochem. 31, 85-89.
11. Banerjee, U. C., Sani, R. K., Azmi, W., and, Soni, R., (1999) Process Biochem. 35, 213-219.
12. Manachini, P. L., and, Fortina, M. G., (1998) Biotechnol. Lett. 20, 565-568.
13. Gupta, A., Roy, I., Patel, R. K., Singh, S. P., Khare, S. K., and, Gupta, M. N., (2005) J. Chromatogr. A 1075, 103-108.
14. Kamoun, A. S., Haddar, A., Ali, N. E. H., Frikha, B. G., Kanoun, S., and, Nasri, M., (2008) Microbiol. Res. 163, 299-306.
15. Maurer, K. H., (2004) Curr. Opin. Biotechnol. 15, 330-334.
16. Sen, S., and, Satyanarayana, T., (1993) Ind. J. Micribiol. 33, 43-47.
17. Paliwal, N., Singh, S. P., and, Garg, S. K., (1994) Bioresour. Technol. 50, 209-211.
18. Najafi, M. F., Deobagkar, D., and, Deobagkar, D., (2005) Electron. J. Biotechnol. 8, 197-203.
19. Kembhavi, A. A., Kulkarni, A., and, Pant, A., (1993) Appl. Biochem. Biotechnol. 38, 83-92.
20. Bradford, M. M. A., (1976) Anal. Biochem. 72, 248-254.
21. Laemmli, U. K., (1970) Nature 227, 680-685.
22. Bernal, C., Vidal, L., Valdivieso, E., and, Coello, N., (2003) World J. Microbiol. Biotechnol. 19, 255-261.
23. Kohlmann, K. L., Nielsen, S. S., Steenson, L. R., and, Ladisch, M. R., (1991) J. Dairy Sci. 74, 3275-3283.
24. Oh, Y. S., Shih, I. L., Tzeng, Y. M., and, Wang, S. L., (2000) Enzyme Microb. Technol. 27, 3-10.
25. Kasana, R. C., and, Yadav, S. K., (2007) Curr. Microbiol. 54, 224-229.
26. Zhu, W., Cha, D., Cheng, G., Peng, Q., and, Shen, P., (2007) Enzyme Microb. Technol. 40, 1592-1597.
27. Romero, F. J., Garcia, L. A., Salas, J. A., Diaz, M., and, Quiros, L. M., (2001) Process Biochem. 36, 507-515.
28. Sookkheo, B., Sinchaikul, S., Phutrakul, S., and, Chen, S. T., (2000) Protein Expr. Purif. 20, 142-151.
29. Yossan, S., Reungsang, A., and, Yasuda, M., (2006) Sci. Asia 32, 377-383.
30. Moreira, K. A., Porto, T. S., Teixeira, M. F. S., Porto, A. L. F., and, Filho, J. L. L., (2003) Process Biochem. 39, 67-72.
31. Tunga, R., Shrivastava, B., and, Banerjee, R., (2003) Process Biochem. 38, 1553-1558.
32. Fujiwara, N., Masui, A., and, Imanaka, T., (1993) J. Biotechnol. 30, 245-256.
33. Nascimento, W. C. A., and, Martins, M. L. L., (2004) Braz. J. Microbiol. 35, 91-96.
34. North, M. J., (1982) Microbiol. Rev. 46, 308-340.
35. Wang, S. L., Chio, Y. H., Yen, Y. H., and, Wang, C. L., (2007) Enzyme Microb. Technol. 40, 1213-1220.
36. Haddar, A., Bougatef, A., Agrebi, R., Kamoun, A. S., and, Nasri, M., (2009) Process Biochem. 44, 29-35.