Quantitative detection and identification of Naegleria spp. in various environmental water samples using real-time quantitative PCR assay

Parasitology Research

Volumn 112, Issue 4, 2013, Pages 1467-1474

  Kao, Po Min ^a,b   Tung, Min Che ^a   Hsu, Bing Mu ^b   Chou, Ming Yuan ^c   Yang, Hsiu Wu ^c   She, Cheng Yu ^b   Shen, Shu Min ^b  

^a TUNGS' TAICHUNG METROHARBOR HOSPITAL   (Taiwan)

^b NATIONAL CHUNG CHENG UNIVERSITY   (Taiwan)

^c CHENG HSIN GENERAL HOSPITAL   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

DRINKING WATER;

ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; MOLECULAR PHYLOGENY; NAEGLERIA; NAEGLERIA AUSTRALIENSIS; NAEGLERIA LOVANIENSIS; NAEGLERIA SPITZBERGENIENSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE IDENTIFICATION; PRIORITY JOURNAL; PROCESS DEVELOPMENT; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RIVER; SEQUENCE ANALYSIS; TAXONOMIC IDENTIFICATION; THERMAL SPRING; WATER ANALYSIS; WATER QUALITY; WATER SAMPLING;

NAEGLERIA; PARASITE LOAD; REAL-TIME POLYMERASE CHAIN REACTION; WATER MICROBIOLOGY;

ANIMALIA; NAEGLERIA; NAEGLERIA AUSTRALIENSIS; NAEGLERIA LOVANIENSIS;

EID: 84876419234     PISSN: 09320113     EISSN: 14321955     Source Type: Journal    
DOI: 10.1007/s00436-013-3290-x     Document Type: Article

References (36)

1. Amorn L, Chantira S, Somchai B, Yaowalark S (2005) Free-living ameba contamination in natural hot springs in Thailand. Southeast Asian J Trop Med Publ Health 36:1-5
2. APHA (2005) Standard method for the examination of water and wastewater. APHA, WEF and AWWA, Washington, DC
3. Behets J, Declerck P, Delaedt Y, Verelst L, Ollevier F (2007a) A duplex real-time PCR assay for the quantitative detection of Naegleria fowleri in water samples. Water Res 41:118-126
4. Behets J, Declerck P, Delaedt Y, Verelst L, Ollevier F (2007b) Survey for the presence of specific free-living amoebae in cooling waters from Belgian power plants. Parasitol Res 100:1249-1256
5. Bej AK (2003) Molecular based methods for the detection of microbial pathogens in the environment. J Microbiol Methods 53:139-140
6. Clark CG, Cross GAM (1988) Small-subunit ribosomal RNA sequence from Naegleria gruberi supports the polyphyletic origin of amoebas. Mol Biol Evol 5:512-518
7. De Jonckheere JF (2006) Isolation and molecular identification of free-living amoebae of the genus Naegleria from Arctic and sub-Antarctic regions. Eur J Protistol 42:115-123
8. De Jonckheere JF (2011) Origin and evolution of the worldwide distributed pathogenic amoeboflagellate Naegleria fowleri. Infect Genet Evol 11:1520-1528
9. Edagawa A, Kimura A, Kawabuchi-Kurata T, Kusuhara Y, Karanis P (2009) Isolation and genotyping of potentially pathogenic Acanthamoeba and Naegleria species from tap-water sources in Osaka, Japan. Parasitol Res 105:1109-1117
10. Ettinger M, Webb S, Harris S, McIninch S, Garman G C, Brown B (2003) Distribution of free-living amoebae in James River, Virginia, USA. Parasitol Res 89:6-15
11. Guzman-Fierros E, De Jonckheere JF, Lares-Villa F (2008) Identification of Naegleria species in recreational areas in Hornos, Sonora. Rev Mex Biodiv 79:1-5
12. Hoffmann R, Michel R (2001) Distribution of free-living amebae (FLA) during preparation and supply of drinking water. Int J Hyg Environ Health 203:215-219
13. Hsu BM, Lin CL, Shih FC (2009) Survey of pathogenic free-living amoebae and Legionella spp. in mud spring recreation area. Water Res 43:2817-2828
14. Hsu BM, Huang CC, Chen JS, Chen NH, Huang JT (2011) Comparison of potentially pathogenic free-living amoeba hosts by Legionella spp. in substrate-associated biofilms and floating biofilms from spring environments. Water Res 45:5171-5183
15. Huang SW, Hsu BM (2010) Survey of Naegleria and its resisting bacteria-Legionella in hot spring water of Taiwan using molecular method. Parasitol Res 106:1395-1402
16. Huang SW, Hsu BM (2011) Survey of Naegleria from Taiwan recreational waters using culture enrichment combined with PCR. Acta Trop 119:114-118
17. Huang SW, Hsu BM, Chen NH, Huang CC, Huang KH, Chen JS, Kao PM (2011) Isolation and identification of Legionella and their host amoebae from weak alkaline carbonate spring water using a culture method combined with PCR. Parasitol Res 109:1233-1241
18. Jamerson M, Remmers K, Cabral G, Marciano-Cabral F (2008) Survey for the presence of Naegleria fowleri amebae in lake water used to cool reactors at a nuclear power generating plant. Parasitol Res 104:969-978
19. Johnson PE, Deromedi AJ, Lebaron P, Catala P, Havens C, Pougnard C (2007) High throughput, real-time detection of Naegleria lovaniensis in natural river water using LED-illuminated fountain flow cytometry. J Appl Microbiol 103:700-710
20. Jonckheere JFD (1998) Sequence variation in the ribosomal internal transcribed spacers, including the 5.8S rDNA, of Naegleria spp. Protist 149:221-228
21. Lares-Villa F, Hernández-Peña C (2010) Concentration of Naegleria fowleri in natural waters used for recreational purposes in Sonora, Mexico (November 2007-October 2008). Exp Parasitol 126:33-36
22. Leiva B, Clasdotter E, Linder E, Winiecka-Krusnell J (2008) Free-living Acanthamoeba and Naegleria spp. amebae in water sources of León, Nicaragua. Rev Biol Trop 56:439-446
23. Mackay IM (2004) Real-time PCR in the microbiology laboratory. Clin Microbiol Infect 10:190-212
24. Maclean R, Richardson D, LePardo R, Marciano-Cabral F (2004) The identification of Naegleria fowleri from water and soil samples by nested PCR. Parasitol Res 93:211-217
25. Pernin P, Pelandakis M, Rouby Y, Faure A, Siclet F (1998) Comparative recoveries of Naegleria fowleri amoebae from seeded river water by filtration and centrifugation. Appl Environ Microb 64:955-959
26. Pougnard C, Catala P, Drocourt JL, Legastelois S, Pernin P, Pringuez E, Lebaron P (2002) Rapid detection and enumeration of Naegleria fowleri in surface waters by solid-phase cytometry. Appl Environ Microb 68:3102-3107
27. Puzon GJ, Lancaster JA, Wylie JT, Plumb IJ (2009) Rapid detection of Naegleria fowleri in water distribution pipeline biofilms and drinking water samples. Environ Sci Technol 43:6691-6696
28. Robinson BS, Monis PT, Dobson PJ (2006) Rapid, sensitive, and discriminating identification of Naegleria spp. by real-time PCR and melting-curve analysis. Appl Environ Microb 72:5857-5863
29. Schild M, Gianinazzi C, Gottstein B, Muller N (2007) PCR-based diagnosis of Naegleria sp. infection in formalin-fixed and paraffin-embedded brain sections. J Clin Microbiol 45:564-567
30. Schuster FL, Visvesvara GS (2004) Free-living amoebae as opportunistic and nonopportunistic pathogens of humans and animals. Int J Parasitol 34:1001-1027
31. Sheehan KB, Fagg JA, Ferris MJ, Henson JM (2003) PCR detection and analysis of the free-living amoeba Naegleria in hot springs in Yellowstone and Grand Teton National Parks. Appl Environ Microb 69:5914-5918
32. Stockman LJ, Wright CJ, Visvesvara GS, Fields BS, Beach MJ (2011) Prevalence of Acanthamoeba spp. and other free-living amoebae in household water, Ohio, USA-1990-1992. Parasitol Res 108:621-627
33. Thomas V, Loret JF, Jousset M, Greub G (2008) Biodiversity of amoebae and amoebae-resisting bacteria in a drinking water treatment plant. Environ Microbiol 10:2728-2745
34. Visvesvara G, Schuster F (2008a) Opportunistic free-living amebae, part I. Clin Microbiol Newsl 30:151-158
35. Visvesvara G, Schuster F (2008b) Opportunistic free-living amebae, part II. Clin Microbiol Newsl 30:159-166
36. Wannasan A, Chaiwong P, Bunchoo M, Morakote N (2009) Occurrence of thermotolerant Naegleria and Acanthamoeba in some natural water sources in Chiang Mai. Chiang Mai Med J 48:117-124