High throughput, real-time detection of Naegleria lovaniensis in natural river water using LED-illuminated Fountain Flow™ Cytometry

Journal of Applied Microbiology

Volumn 103, Issue 3, 2007, Pages 700-710

  Johnson, P E ^a,b,c   Deromedi, A J ^a,b   Lebaron, P ^c   Catala, P ^c   Havens, C ^a   Pougnard, C ^d  

^a UNIVERSITY OF WYOMING   (United States)

^b SoftRay Inc   (United States)

^c Laboratoire d Océanographie Microbienne   (France)

^d EDF R AND D   (France)

Author keywords

Cell detection; Environmental monitoring; Flow cytometry; Naegleria lovaniensis; Protozoa detection; Water monitoring

Indexed keywords

FLUORESCENCE; FOUNTAINS; POTABLE WATER; PROTOZOA; RIVERS; SIGNAL DETECTION;

CELL DETECTION; ENVIRONMENTAL MONITORING; FLUORESCENT LABELS; FOUNTAIN FLOW; NAEGLERIUM LOVANIENSIS; NATURAL RIVER; PROTOZOA DETECTION; REAL-TIME DETECTION; RIVER WATER; WATER MONITORING;

FLOW CYTOMETRY;

ANTIBODY; DRINKING WATER; FLUORESCENT DYE; INDICATOR; R PHYCOERYTHERIN; RIVER WATER; UNCLASSIFIED DRUG; WATER;

DETECTION METHOD; DIGITAL IMAGE; DRINKING WATER; FLOW CYTOMETRY; FRESHWATER ENVIRONMENT; PROTOZOAN; PUBLIC HEALTH; REAL TIME; SANITATION;

AMOEBA (LIFE CYCLE STAGE); ANTIBODY LABELING; ARTICLE; ATTENUATION; CAMERA; CONCENTRATION (PARAMETERS); ENVIRONMENT; FLOW CYTOMETRY; FLOW RATE; FLUORESCENCE; FRANCE; FUNGAL DETECTION; ILLUMINATION; INOCULATION; LIGHT EMITTING DIODE; LIQUID; NAEGLERIA; NAEGLERIA FOWLERI; NAEGLERIA LOVANIENSIS; NONHUMAN; PARASITE VIABILITY; PATHOGENESIS; PROTOZOON; PUBLIC HEALTH; RIVER; SAMPLE; SANITATION; SOLID; UNITED STATES;

EURASIA; EUROPE; FRANCE; NORTH AMERICA; UNITED STATES; WESTERN EUROPE;

NAEGLERIA FOWLERI; NAEGLERIA LOVANIENSIS; PROTOZOA;

EID: 34547885396     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2007.03307.x     Document Type: Article

References (13)

1. De Jonckheere, J. (1977) Use of an axenic medium for differentiation between pathogenic and non-pathogenic Naegleria fowleri isolates. Appl Environ Microbiol 33, 751 757.
2. Ford, T.E. (1999) Microbiological safety of drinking water: United States and global perspectives. Environ Health Perspect 107, 191 206.
3. Invitrogen Corporation (2005) The Handbook - A Guide to Fluorescent Probes and Labeling Technologies. [Online.] Available at http://probes. invitrogen.com/handbook.
4. Johnson, P.E. (2004) Apparatus and methods for high throughput analysis of samples in a translucent flowing liquid. U.S. Patent No. 6,765,656.
5. Johnson, P.E. (2006) Method and system for counting particles in a laminar flow with an imaging device. U.S. Patent Application No. 11/328,033.
6. Johnson, P.E., Votaw, A.S. Deromedi, A.J. (2002) Biodetection with flow cytometry: better, faster, cheaper. In Biodetection Technologies, Vol. 1. pp. 71 83. Massachusetts : Brookline.
7. TM Cytometry. Cytometry Part A 69A, 1212 1221.
8. Parthuisot, N., Catala, P., Lemarchand, K., Baudart, J. Lebaron, P. (2000) Evaluation of ChemChrome V6 for bacterial viability assessment in waters. J Appl Microbiol 89, 370 380.
9. Pougnard, C., Catala, P., Drocourt, J.L., Legastelois, S., Pernin, P., Pringuez, E. Lebaron, P. (2002) Rapid detection and enumeration of Naegleria fowleri in surface waters by solid-phase cytometry. Appl Environ Microbiol 68, 3102 3107.
10. Sluter, S.D., Tzipori, S. Widmer, G. (1997) Parameters affecting polymerase chain reaction detection of waterborne Cryptosporidium parvum oocysts. Appl Microbiol Biotechnol 48, 325 330.
11. Walner, G., Tillman, D., Haberer, K., Cornet, P. Drocourt, J.L. (1997) The ChemScan system: a new method for rapid microbiological testing of water. Eur J Parenter Sci 2, 123 126.
12. WHO/OEDC (2003) Assessing the Microbial Safety of Drinking Water. Geneva : World Health Organization.
13. WHO/UNICEF (2002) Global Water Supply and Sanitation Assessment 2000 Report. Geneva : World Health Organization.