Determination of gymnemagenin in rat plasma using high-performance liquid chromatography-tandem mass spectrometry: Application to pharmacokinetics after oral administration of Gymnema sylvestre extract

Biomedical Chromatography

Volumn 27, Issue 5, 2013, Pages 669-675

  Kamble, Bhagyashree ^a   Gupta, Ankur ^a   Patil, Dada ^b   Khatal, Laxman ^c   Janrao, Shirish ^c   Moothedath, Ismail ^a   Duraiswamy, Basavan ^a  

^a JSS COLLEGE OF PHARMACY   (India)

^b SYMBIOSIS INTERNATIONAL DEEMED UNIVERSITY   (India)

^c Navi Peth Maharashtra   (India)

Author keywords

Anti diabetic; Gymnema sylvestre; Gymnemic acids; HPLC ESI MS MS; Pharmacokinetics

Indexed keywords

BODY FLUIDS; EXTRACTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; RATS;

ANTIDIABETIC; GYMNEMA SYLVESTRE; GYMNEMIC ACIDS; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY; HPLC-ESI-MS/MS; HPLC-MS-MS; HPLC/ESI-MS/MS; INTERNAL STANDARDS; ORAL ADMINISTRATION; RAT PLASMA;

PHARMACOKINETICS;

GYMNEMA SYLVESTRE EXTRACT; GYMNEMAGENIN; SAPOGENIN; UNCLASSIFIED DRUG; WITHAFERIN A;

ANIMAL EXPERIMENT; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; DRUG BLOOD LEVEL; DRUG DETERMINATION; DRUG STRUCTURE; GYMNEMA SYLVESTRE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUID LIQUID EXTRACTION; MALE; MAXIMUM PLASMA CONCENTRATION; NONHUMAN; PLASMA CONCENTRATION-TIME CURVE; RAT; REACTION ANALYSIS; TANDEM MASS SPECTROMETRY;

GYMNEMA SYLVESTRE; RATTUS; TETRA;

EID: 84876319673     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.2845     Document Type: Article

References (21)

1. Baskaran K, Ahamath BK, Shanmugasundaram KR and Shanmugasundaram ERB. Antidiabetic effect of a leaf extract from Gymnema sylvestre in non-insulin dependent Diabetes mellitus patients. Journal of Ethnopharmacology 1990; 30: 295-305.
2. Cai F, Sun L, Gao S, Yanga Y, Yanga Q and Chena W. A rapid and sensitive liquid chromatography-tandem mass spectrometric method for the determination of timosaponin B-II in blood plasma and a study of the pharmacokinetics of saponin in the rat. Journal of Pharmaceutical and Biomedical Analysis 2008; 48: 1411-1416.
3. Chodisetti B, Rao K, Giri A. Phytochemical analysis of Gymnema sylvestre and evaluation of its antimicrobial activity. Nat Prod Res 2012; DOI:10.1080/14786419.2012.676548 (in press).
4. Han WJ, Xia YF and Dai Y. Development and validation of high-performance liquid chromatography/electrospray ionization mass spectrometry for assay of madecassoside in rat plasma and its application to pharmacokinetic study. Biomedical Chromatography 2012; 26: 26-32.
5. Imoto T, Yamamoto FM, Miyasaka A and Hatano H. High-performance liquid chromatography-atmospheric pressure ionization mass spectrometry of gymnemic acids. Journal of Chromatography 1991; 557: 383-389.
6. Indian Pharmacopoeia Commission. Indian Pharmacopoeia, Vol. 3. Indian Pharmacopoeia Commission, Ministry of Health & Family welfare, Government of India: Ghaziabad, 2007; 2036-2037.
7. Kamble B, Gupta A, Patil D, Janrao S, Khatal L and Duraiswamy B. Quantitative estimation of gymnemagenin in Gymnema sylvestre extract and its marketed formulations using HPLC-ESI-MS/MS Method. Phytochemical Analysis 2012; doi: 10.1002/pca.2392 (in press).
8. Kanetkar P, Singhal R and Kamat M. Gymnema sylvestre: a memoir. Journal of Clinical Biochemistry and Nutrition 2005; 41: 77-81.
9. Kang MH, Lee MS, Choi MK, Min KS and Shibamoto T. Hypoglycemic activity of Gymnema sylvestre extracts on oxidative stress and antioxidant status in diabetic rats. Journal of Agriculture and Food Chemistry 2012; 60: 2517-2524.
10. Lavie D, Glotter E and Shvo Y. Constituents of Withania somnifera Dun. Part IV. The structure of withaferin A. Journal of American Chemical Society 1965; 30: 7517-7531.
11. Rachh PR, Rachh MR, Ghadiya NR, Modi DC, Patel NM and Rupareliya MT. Antihyperlipidimic activity of Gymnema sylvestre leaf extract on rats fed with high cholesterol diet. International Journal of Pharmacology 2010; 6: 138-141.
12. Saneja A, Sharma C, Aneja KR and Pahwa R. Gymnema Sylvestre (Gurmar): a review. Der Pharmacia Lettre 2010; 2: 275-284.
13. Shakya S, Zhu H, Ding L, Du XL, Qi XM, Yang XL and Yang ZL. Determination of asperosaponin VI in dog plasma by high-performance liquid chromatography-tandem mass spectrometry and its application to a pilot pharmacokinetic study. Biomedical Chromatography 2012; 26: 109-114.
14. Shanmugasundaram ERB, Gopinath KL, Shanmugasundaram KR and Rajendran VM. Possible regeneration of the islets of Langerhans in streptozotocin-diabetic rats given Gymnema Sylvestre leaf extracts. Journal of Ethnopharmacology 1990; 30: 265-279.
15. Srikanth AV, Maricar S, Narsu ML, Kumar PR and Reddy M. Anticancer activity of Gymnema sylvestre R.Br. International Journal of Pharmaceutical Sciences and Nanotechnology 2010; 3: 897-899.
16. Takeda S, Ishihara K, Wakui Y, Amagaya S, Maruno M, Akao T and Kobashi K. Bioavailability study of glycyrrhetic acid after oral administration of glycyrrhizin in rats; relevance to the intestinal bacterial hydrolysis. Journal of Pharmacy and Pharmacology 1996; 48: 902-905.
17. Toshihiro Y, Kenzi M, Kenzo O and Osamu T. Quantitative analysis of gymnemic acids by high performance liquid chromatography. Nippon Shokuhin Kagaku Kaishi 1994; 41: 202-205.
18. Trivedi PD, Pundarikakshudu K and Shah KA. Validated reverse phase liquid chromatographic method for quantification of gymnemagenin in the Gymnema sylvestre R. Br. leaf samples, extract and market formulation. International Journal of Applied Sciences and Engineering 2011; 9: 25-31.
19. US Food and Drug Administration. FDA Guidance for Industry: Bioanalytical Method Validation. US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research: Rockville, MD, 2001.
20. Valivarthi SRR, Kannababu S and Gottumukkala VS. Standardisation of Gymnema sylvestre R. Br. by high-performance thin-layer chromatography: an improved method. Phytochemical Analysis 2006; 17: 192-196.
21. Yoshikaua K, Alimotoa K, Ariharaa S and Natsuura K. Structure studies of new antisweet constituents from Gymnema sylvestre. Tetrahedron Letters 1989; 30: 1103-1106.