Characterisation of a thermo-alkali-stable lipase from oil-contaminated soil using a metagenomic approach

Systematic and Applied Microbiology

Volumn 36, Issue 3, 2013, Pages 197-204

  Zheng, Jianhua ^a   Liu, Cuina ^b   Liu, Liguo ^a   Jin, Qi ^a  

^a INSTITUTE OF PATHOGEN BIOLOGY   (China)

^b RENMIN UNIVERSITY OF CHINA   (China)

Author keywords

Expression; Gene cloning; Lipase; Metagenomic library; Pichia pastoris

Indexed keywords

BIODIESEL; CALCIUM; CARBON; MANGANESE; METHANOL; OLIVE OIL; TRIACYLGLYCEROL; TRIACYLGLYCEROL LIPASE;

ALKALINITY; ARTICLE; ELECTROPHORESIS; ENZYME ACTIVITY; ENZYME SPECIFICITY; GENE; LIPZ01 GENE; METAGENOMICS; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PICHIA PASTORIS; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SOIL POLLUTION; TEMPERATURE; TRANSESTERIFICATION;

ALKALIES; CLONING, MOLECULAR; ENVIRONMENT; ENZYME ACTIVATION; ENZYME STABILITY; GENE EXPRESSION; GENE LIBRARY; HYDROGEN-ION CONCENTRATION; LIPASE; METAGENOMICS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SOIL MICROBIOLOGY; SUBSTRATE SPECIFICITY; TEMPERATURE;

PICHIA PASTORIS;

EID: 84876313970     PISSN: 07232020     EISSN: 16180984     Source Type: Journal    
DOI: 10.1016/j.syapm.2012.12.008     Document Type: Article

References (34)

1. Bunterngsook B., Kanokratana P., Thongaram T., Tanapongpipat S., Uengwetwanit T., Rachdawong S., Vichitsoonthonkul T., Eurwilaichitr L. Identification and characterization of lipolytic enzymes from a peat-swamp forest soil metagenome. Biosci. Biotechnol. Biochem. 2010, 74:1848-1854.
2. Cieslinski H., Bialkowskaa A., Tkaczuk K., Dlugolecka A., Kur J., Turkiewicz M. Identification and molecular modeling of a novel lipase from an Antarctic soil metagenomic library. Pol. J. Microbiol. 2009, 58:199-204.
3. Cote A., Shareck F. Expression and characterization of a novel heterologous moderately thermostable lipase derived from metagenomics in Streptomyces lividans. J. Ind. Microbiol. Biotechnol. 2010, 37:883-891.
4. Cregg J.M., Cereghino J.L., Shi J., Higgins D.R. Recombinant protein expression in Pichia pastoris. Mol. Biotechnol. 2000, 16:23-52.
5. 4 DNA polymerase replacement synthesis for specific labeling of plasmid-cloned inserts. Anal. Biochem. 1983, 135:456-465.
6. Duong F., Soscia C., Lazdunski A., Murgier M. The Pseudomonas fluorescens lipase has a C-terminal secretion signal and is secreted by a three-component bacterial ABC-exporter system. Mol. Microbiol. 1994, 11:1117-1126.
7. Glogauer A., Martini V.P., Faoro H., Couto G.H., Muller-Santos M., Monteiro R.A., Mitchell D.A., de Souza E.M., Pedrosa F.O., Krieger N. Identification and characterization of a new true lipase isolated through metagenomic approach. Microb. Cell Fact. 2011, 10:54.
8. Henne A., Schmitz R.A., Bomeke M., Gottschalk G., Daniel R. Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli. Appl. Environ. Microbiol. 2000, 66:3113-3116.
9. Hide W.A., Chan L., Li W.H. Structure and evolution of the lipase superfamily. J. Lipid Res. 1992, 33:167-178.
10. Jeon J.H., Kim J.T., Kim Y.J., Kim H.K., Lee H.S., Kang S.G., Kim S.J., Lee J.H. Cloning and characterization of a new cold-active lipase from a deep-sea sediment metagenome. Appl. Microbiol. Biotechnol. 2009, 81:865-874.
11. Jin H., Liu G., Dai K., Wang H., Li Z., Shi Z. Improvement of porcine interferon-alpha production by recombinant Pichia pastoris via induction at low methanol concentration and low temperature. Appl. Biochem. Biotechnol. 2011, 165:559-571.
12. Kim H.K., Park S.Y., Lee J.K., Oh T.K. Gene cloning and characterization of thermostable lipase from Bacillus stearothermophilus L1. Biosci. Biotechnol. Biochem. 1998, 62:66-71.
13. Kouker G., Jaeger K.E. Specific and sensitive plate assay for bacterial lipases. Appl. Environ. Microbiol. 1987, 53:211-213.
14. Krsek M., Wellington E.M. Comparison of different methods for the isolation and purification of total community DNA from soil. J. Microbiol. Methods 1999, 39:1-16.
15. Lan D.M., Yang N., Wang W.K., Shen Y.F., Yang B., Wang Y.H. A novel cold-active lipase from Candida albicans: cloning, expression and characterization of the recombinant enzyme. Int. J. Mol. Sci. 2011, 12:3950-3965.
16. Lee M.H., Lee C.H., Oh T.K., Song J.K., Yoon J.H. Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacterial lipases. Appl. Environ. Microbiol. 2006, 72:7406-7409.
17. Lee Y.P., Chung G.H., Rhee J.S. Purification and characterization of Pseudomonas fluorescens SIK W1 lipase expressed in Escherichia coli. Biochim. Biophys. Acta 1993, 1169:156-164.
18. Li G., Wang K., Liu Y.H. Molecular cloning and characterization of a novel pyrethroid-hydrolyzing esterase originating from the metagenome. Microb. Cell Fact. 2008, 7:38.
19. Lorenz P., Liebeton K., Niehaus F., Eck J. Screening for novel enzymes for biocatalytic processes: accessing the metagenome as a resource of novel functional sequence space. Curr. Opin. Biotechnol. 2002, 13:572-577.
20. Luo Y., Zheng Y., Jiang Z., Ma Y., Wei D. A novel psychrophilic lipase from Pseudomonas fluorescens with unique property in chiral resolution and biodiesel production via transesterification. Appl. Microbiol. Biotechnol. 2006, 73:349-355.
21. Martin-Laurent F., Philippot L., Hallet S., Chaussod R., Germon J.C., Soulas G., Catroux G. DNA extraction from soils: old bias for new microbial diversity analysis methods. Appl. Environ. Microbiol. 2001, 67:2354-2359.
22. Meilleur C., Hupe J.F., Juteau P., Shareck F. Isolation and characterization of a new alkali-thermostable lipase cloned from a metagenomic library. J. Ind. Microbiol. Biotechnol. 2009, 36:853-861.
23. Minning S., Serrano A., Ferrer P., Sola C., Schmid R.D., Valero F. Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. J. Biotechnol. 2001, 86:59-70.
24. Ogawa J., Shimizu S. Microbial enzymes: new industrial applications from traditional screening methods. Trends Biotechnol. 1999, 17:13-21.
25. Quyen D.T., Schmidt-Dannert C., Schmid R.D. High-level formation of active Pseudomonas cepacia lipase after heterologous expression of the encoding gene and its modified chaperone in Escherichia coli and rapid in vitro refolding. Appl. Environ. Microbiol. 1999, 65:787-794.
26. Roh C., Villatte F. Isolation of a low-temperature adapted lipolytic enzyme from uncultivated micro-organism. J. Appl. Microbiol. 2008, 105:116-123.
27. Rosenau F., Jaeger K. Bacterial lipases from Pseudomonas: regulation of gene expression and mechanisms of secretion. Biochimie 2000, 82:1023-1032.
28. San Clemente C.L., Vadehra D.V. Instrumental assay of microbial lipase at constant pH. Appl. Microbiol. 1967, 15:110-113.
29. Shi B., Zeng L., Song H., Shi Q., Wu S. Cloning and expression of Aspergillus tamarii FS132 lipase gene in Pichia pastoris. Int. J. Mol. Sci. 2010, 11:2373-2382.
30. Stratton J., Chiruvolu V., Meagher M. High cell-density fermentation. Methods Mol. Biol. 1998, 103:107-120.
31. Taher H., Al-Zuhair S., Al-Marzouqi A.H., Haik Y., Farid M.M. A review of enzymatic transesterification of microalgal oil-based biodiesel using supercritical technology. Enzyme Res. 2011, 2011:468292.
32. Tebbe C.C., Vahjen W. Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast. Appl. Environ. Microbiol. 1993, 59:2657-2665.
33. Yang J., Zhang B., Yan Y. Cloning and expression of Pseudomonas fluorescens 26-2 lipase gene in Pichia pastoris and characterizing for transesterification. Appl. Biochem. Biotechnol. 2009, 159:355-365.
34. Yeates C., Gillings M.R., Davison A.D., Altavilla N., Veal D.A. Methods for microbial DNA extraction from soil for PCR amplification. Biol. Proced. Online 1998, 1:40-47.