Simple and robust DNA extraction method for the large-scale analysis of genotypes containing high polyphenolic content, such as landraces of Solanum tuberosum and Zea mays;Método simple y robusto de extracción de ADN para análisis a gran escala de genotipos con alto contenido de polifenoles, como son variedades nativas de Solanum tuberosum and Zea mays

Ciencia e Investigacion Agraria

Volumn 39, Issue 3, 2012, Pages 593-601

  Barra, Macarena ^a,b   Salazar, Erika ^a,c   Beltrán, María ^d   Sagredo, Boris ^a  

^a INSTITUTO DE INVESTIGACIONES AGROPECUARIAS   (Chile)

^b UNIVERSIDAD DE LA FRONTERA   (Chile)

^c PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

^d UNIVERSITY OF CONCEPCIÓN   (Chile)

Author keywords

Activated charcoal; DNA extraction; Genomic DNA; Polyphenols

Indexed keywords

SOLANACEAE; SOLANUM TUBEROSUM; ZEA MAYS;

EID: 84875989729     PISSN: 03045609     EISSN: 07181620     Source Type: Journal    
DOI: 10.4067/S0718-16202012000300019     Document Type: Article

References (21)

1. Bashalkhanov, S., and O. Rajora. 2008. Protocol: A high-throughput DNA extraction system suitable for conifers. Plant Methods 4:20.
2. Bennett, R.N., and R.M. Wallsgrove. 1994. Secondary metabolites in plant defence mechanisms. New Phytologist 127:617-633.
3. Bi, I.V., L. Harvengt, A. Chandelier, G. Mergeai, P. Du Jardin. 1996. Improved RAPD amplification of recalcitrant plant DNA by the use of activated charcoal during DNA extraction. Plant Breeding 115:205-206.
4. Boiteux, L.S., M.E.N. Fonseca, and P.W. Simon. 1999. Effects of plant tissue and DNA purification method on randomly amplified polymorphic DNA-based genetic fingerprinting analysis in Carrot. Journal of the American Society for Horticultural Science 124:32-38.
5. CIMMYT. 2005. Laboratory Protocols. CIMMYT Applied Molecular Genetics Laboratory. Third Edition. Mexico. D.F. 102 pp.
6. Friar E, A. 2005. Isolation of DNA from plants with large amounts of secondary metabolites methods in enzymology. Methods Enzymol. 395:3-14.
7. Fulton, T., J. Chunwongse, and S. Tanksley. 1995. Microprep protocol for extraction of DNA from tomato and other herbaceous plants. Plant Molecular Biology Reporter 13:207-209.
8. Hidenori, S. 2007. A technique to isolate DNA from woody and herbaceous plants by using a silicabased plasmid extraction column. Anal. Biochem. 363.166-167.
9. Hollingsworth, M. 2006. DNA fingerprinting in plants: principles, methods, and applications. Annals of Botany 97:476-477.
10. Huamán, Z., and D.M. Spooner. 2002. Reclassification of landrace populations of cultivated potatoes (Solanum sect. Petota). American Journal of Botany 89:947-965.
11. Keb-Llanes, M., G. González, B. Chi-Manzanero, D. Infante. 2002. A rapid and simple method for small-scale DNA extraction in Agavaceae and other tropical plants. Plant Molecular Biology Reporter 20:299-299.
12. Križman, M., J. Jakše, D. Baričevič, B. Javornik, and P. Mirko. 2006. Robust CTAB-activated charcoal protocol for plant DNA extraction. Acta Agriculturae Slovenica 87:427-433.
13. Miller, D.N., J.E. Bryant, E.L. Madsen, and W.C. Ghiorse. 1999. Evaluation and Optimization of DNA Extraction and Purification Procedures for Soil and Sediment Samples. Appl. Environ. Microb. 65.4715-4724.
14. Moyo, M., S.O. Amoo, M.W. Bairu, J.F. Finnie, V.S.J. Optimising. 2008. DNA isolation for medicinal plants. South African Journal of Botany 4:771-775.
15. Mullins, E., D. Milbourne, C. Petti, B.M. Doyle-Prestwich, and C. Meade. 2006. Potato in the age of biotechnology. Trends in Plant Science 11:254-260.
16. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum 15:473-497.
17. Porebski, S., L. Bailey, and B. Baum. 1997. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Molecular Biology Reporter 15:8-15.
18. Ranilla, L.G., E. Apostolidis, M.I. Genovese, F.M. Lajolo, and K. Shetty. 2009. Evaluation of indigenous grains from the Peruvian Andean Region for antidiabetes and antihypertension potential using in vitro methods. Journal of Medicinal Food 12:704-713.
19. Ranilla, L.G., M.I. Genovese, and F.M. Lajolo. 2006. Polyphenols and antioxidant capacity of seed coat and cotyledon from Brazilian and Peruvian bean cultivars (Phaseolus vulgaris L.). J. Agr. Food Chem. 55:90-98.
20. Smith, D.S., P.W. Maxwell, and S.H. De Boer. 2005. Comparison of several methods for the extraction of DNA from potatoes and potato-derived products. J. Agr. Food Chem. 53:9848-9859.
21. Smith, J.S.C., E.C.L. Chin, H. Shu, O.S. Smith, S.J. Wall, M.L. Senior, S.E. Mitchell, S. Kresovich, and J. Ziegle. 1997. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPS and pedigree. TAG Theoretical and Applied Genetics 95:163-173.