Evaluation of antibacterial potential of plant extracts using Resazurin based microtiter dilution assay

International Journal of Pharmacy and Pharmaceutical Sciences

Volumn 5, Issue 2, 2013, Pages 372-376

  Gahlaut, Anjum ^b   Chhillar, Anil K ^a  

^a MAHARSHI DAYANAND UNIVERSITY   (India)

^b NONE

Author keywords

Microtiter dilution assay; Resazurin

Indexed keywords

EMBLICA OFFICINALIS EXTRACT; PICRORHIZA KURROA EXTRACT; PLANT EXTRACT; RESAZURIN; TINOSPORA CORDIFOLIA EXTRACT;

ACACIA CATECHU; ANTIBACTERIAL ACTIVITY; ARTICLE; BACILLUS SUBTILIS; BACTERIUM CULTURE; BERBERIS ARISTATA; CISSUS QUADRANGULARIS; CONTROLLED STUDY; DATURA METEL; EMBLICA OFFICINALIS; ESCHERICHIA COLI; KLEBSIELLA PNEUMONIAE; MICROTITER PLATE ASSAY; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PICRORHIZA KURROA; PONGAMIA PINNATA; PSEUDOMONAS AERUGINOSA; SALMONELLA TYPHI; SENNA TORA; STAPHYLOCOCCUS AUREUS; TINOSPORA CORDIFOLIA;

EID: 84875941178     PISSN: None     EISSN: 09751491     Source Type: Journal    
DOI: None     Document Type: Article

References (43)

1. Gahlaut A, Pawar SD, Mandal TK, Dabur R. Biochemical analysis of lithiasis patients and treatment study using Bryophyllum pinnatum salisb. Int J Pharmacy and Pharma Sci 2012; 4: 505-507.
2. Koppula S, Ammani K, Bobbarala V, Bramhachari PV. Antibacterial activity screening of few medicinal plants from the southern region of India. Journal of Pharma Res 2010; 3: 2453-2456.
3. Clark AM. Natural Products as a resource for new drugs. Pharma Res 1996; 13: 1133-1141.
4. Jain SP, Verma DM, Singh SC, Singh JS, Kumar S. Flora of Haryana. Central Institute of Medicinal and Aromatic plants. Lucknow, India 2000.
5. Rathee D, Rathee P, Rathee S, Rathee D. Phytochemical screening and antimicrobial activity of Picrorrhiza kurroa, an Indian traditional plant used to treat chronic diarrhea. Arab J of Chem 2012, Article in Press.
6. Rajaprabhu D, Rajesh R, Jeyakumar R, Buddhan S, Ganesan B, Anandan R. Protective effect of Picrorhiza kurroa on antioxidant defense status in adriamycin-induced cardiomyopathy in rats. J of Med Plant Res 2007; 1: 080-085.
7. Husain GM, Singh PN, Kumar V. Antidiabetic activity of standardized extract of Picrorhiza kurroa in rat model of NIDDM. Drug Discov Ther 2009; 3: 88-92.
8. Dabur R, Diwedi SK, Yadav V, Mishra V, Singh R, Singh H, Sharma GL. Efficacy of 2-(3,4-dimethyl-2,5-dihydro-1H-pyrrole-2-yl)-1-methylethyl pentanoate in a murine model of invasive aspergillosis. Antimicrob Agen and Chemotherap 2005; 49: 4365-4367.
9. Dabur R, Chhillar AK, Yadav V, Kamal PK, Gupta J, Sharma GL. In vitro antifungal activity of 2-(3,4-dimethyl-2,5-dihydro-1H-pyrrol-2-yl)-1-methylethyl pentanoate, a dihydropyrrole derivative. J of Med Microbio 2005; 54: 549-552.
10. Rajesh, Sharma GL. Studies on antimycotic properties of Datura metel. J of Ethnopharmaco 2002; 80: 193-197.
11. Negi BS, Dave BP. In Vitro Antimicrobial Activity of Acacia catechu and Its Phytochemical Analysis. Indian J Microbiol 2010; 50: 369-374.
12. Ismail S, Asad M. Immunomodulatory activity of Acacia catechu. Indian J Physiol Pharmacol 2009; 53: 25-33.
13. Lakshmi. T, Roy A, Geetha RV. "Acacia catechu Willd-A gift from ayurveda to mankind"-A review. The Pharma Res 2011; 5: 273-293.
14. Nagaraja TG, Sarang SV, Jambhale DC. Evaluation of anti-mycotic activity of Acacia catechu Willd. (Mimosaceae). J of Biopesticides 2008; 1: 197-198.
15. Udupa KN, Prasad GC, Sen SP. The effect of phytogenic steroid in the accleration of fracture repair. Life science 1965; 4: 317.
16. Jainu M, Devi CSS. Effect of Cissus quadrangularis on gastric mucosal defensive factors in experimentally induced gastric ulcer-A comparative study with sucralfate. J Med Food 2004; 7: 372-376.
17. Singh LM, Udupa KN. Studies of C. quadrangularis in fracture by using phosphorus. Ind J Med Sci 1962; 76: 926-931.
18. Luseba D, Elgorashi EE, Ntloedibe DT, Staden JV. Antibacterial, anti-inflammatory and mutagenic effects of some medicinal plants used in South Africa for the treatment of wounds and retained placenta in livestock. South African J of Bot2007; 73: 378-83.
19. Rajpal V. Standardization of Botanicals. Eastern Publishers 2005; 1: 77-81.
20. Tiwari P, Kumar K, Panik R, et al. Hepatoprotective effects of Cassia tora whole plant. Inter Jof Pharma and Tech 2011; 3: 2798-2806.
21. Yen GC, Wu CH. Antigenotoxic properties of Cassia tea (Cassia tora L.): Mechanism of action and the influence of roasting process. Life Sci 2004; 76: 85-101.
22. Hyun SK, Lee H, Kang SS, Chung HY, Choi JS. Inhibitory activities of Cassia tora and its anthraquinone constituents on angiotensin converting enzyme. Phytother Res 2009; 23: 178-184.
23. Patel RP, Patel KC. Antibacterial activity of Cassia tora and Cassia obovata. Ind J of Pharma 1957; 19: 70-73.
24. Pal M, Mukherjee PK, Saha K, Saha BP. Antifungal activities of the leaf extract of Cassia tora Linn. (Fam. Leguminosae). Phytother Res 1996; 10: 521-522.
25. Sharma C, Aneja KR, Kasera R. Screening of Berberis aristata for antimicrobial potential against the pathogens causing ear infection. Int J of Pharmacol 2011; 7: 536-541.
26. Shahid M, Rahim T, Shahzad A, Tajuddin, Latif A, Fatma T, Rashid M, Raza A, Mustafa S. Ethnobotanical studies on Berberis aristata DC. root extracts. Af J of Biotechnol 2009; 8: 556-563.
27. Das S, Das MK, Mazumder PM, Das S, Basu SP. Cytotoxic Activity of Methanolic Extract of Berberis aristata DC on Colon Cancer. Glob J of Pharma 2009; 3: 137-140.
28. Srinivasan K, Muruganandan S, Lal J. Evaluation of antiinflammatory activity of Pongamia pinnata leaves in rats. J Ethnopharmacology 2001; 78: 151-157.
29. Mahli SS, Basu SP, Sinha KP, Banarjee NC. Pharmacological effects of Karanjin and Pongamol. Ind J of Animal Sc 1989; 59: 657-660.
30. Chandrashekar KS, Prasanna KS. Antimicrobial activity of Pongamia pinnata leaves. Int J Med Res 2010; 1: 18-20.
31. Javale P, Sabnis S. Antimicrobial properties and phytochemical analysis of Emblica officinalis. Asian J Exp Biol Sci 2010: 91-95.
32. Prathapana A, Lijo Cheriana, O, Nampoothiria SV, Minib S, Raghu KG. In vitro antiperoxidative, free radical scavenging and xanthine oxidase inhibitory potentials of ethyl acetate fraction of Saraca ashoka flowers. Nat Product Res 2011; 25: 298-309.
33. Seetharam N, Sujeeth H, Jyothishwaran G, Barad A, Sharanabasappa G, Shabana P. Antibacterial activity of Saraca asoka bark. Ind J P Sci 2003; 4: 658-659.
34. Varghese CD, Satish NC, Pannikar KR. Potential anticancer activity of Saraca asoca extracts towards transplantable tumours in mice. Ind J P Sci 1992; 54: 37-40.
35. Gahlaut A, Taneja P, Shirolkar A, Nale A, Hooda V, Dabur R. Principal component and partial least square discriminant based analysis of methanol extracts of bark and re-generated bark of Saraca Asoca. Int J of Pharma and Pharmaceut Sci 2012; 4: 331-335.
36. Dabur R, Gupta A, Mandal TK, Singh DD, Bajpai V, Gurav AM, Lavekar GS. Antimicrobial activity of some medicinal plants. Af J of Trad, Complement and Alt Med 2007; 4: 313-318.
37. Shirolkar A, Gahlaut A, Chhillar A, Dabur R. Quantitative analysis of catechins in Saraca asoca and correlation with antimicrobial activity. J of Pharma Anal 2013. http://dx.doi.org/10.1016/j.jpha.2013.01.007.
38. Kedar NP, William CC, Bipin K. Multiple antioxidants in prevention and treatment of Parkinson's disease. J of Am Coll of Nutr 1999; 18: 413-23.
39. Gahlaut A, Gothwal A, Dabur R. TLC based analysis of allelopathic effects on tinosporoside contents in Tinospora cordifolia. J of Chem and Pharma Res 2012; 4: 3082-3088.
40. Shirolkar A, Gahlaut A, HoodaV, Dabur R. Phytochemical composition changes in untreated stem juice of Tinospora cordifolia (W) Mier during refrigerated storage. J of Pharma Res 2013. http://dx.doi.org/10.1016/j.jopr.2013.01.018.
41. Sarkar DS, Lutfun N, Kumara Y. Microtitre plate-based antibacterial assay incorporating rezazurin as an indicator of cell growth and its application in the in vitro antibacterial screening of phytochemicals. Methods 2007; 42: 321-4.
42. McNicholl BP, McGrath JW, Quinn JP. Development and application of a resazurin-based biomass activity test for activated sludge plant management. Water Res. 2006; 41: 127-133.
43. Bigalke DL. Methods used for monitoring the microbiological quality of raw milk. Dairy Food Sanit 1984; 4: 189-190.