Development and application of a resazurin-based biomass activity test for activated sludge plant management

Water Research

Volumn 41, Issue 1, 2007, Pages 127-133

  McNicholl, Bridget P ^a   McGrath, John W ^b   Quinn, John P ^b  

^a Bretland House   (United Kingdom)

^b QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

Author keywords

Activated sludge; Biomass activity measurement; Resazurin

Indexed keywords

BIOMASS; COLORIMETRY; COST EFFECTIVENESS; METABOLISM; OPTIMIZATION; WASTEWATER TREATMENT;

BIOMASS ACTIVITY MEASUREMENT; RESAZURIN;

ACTIVATED SLUDGE PROCESS;

RESAZURIN;

ACTIVATED SLUDGE PROCESS; BIOMASS; COLORIMETRY; COST EFFECTIVENESS; METABOLISM; OPTIMIZATION; WASTEWATER TREATMENT;

ACTIVATED SLUDGE; BIOMASS; DYE; METABOLISM; REDUCTION;

ACTIVATED SLUDGE; AEROBIC BACTERIA; ARTICLE; BACTERIAL STRAIN; BIOMASS; COLORIMETRY; CONCENTRATION RESPONSE; CONTROLLED STUDY; EFFLUENT; METABOLIC RATE; NONHUMAN; OXYGEN CONSUMPTION; PRIORITY JOURNAL; PROCESS CONTROL; WASTE WATER MANAGEMENT;

EID: 33751540916     PISSN: 00431354     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.watres.2006.10.002     Document Type: Article

References (23)

1. Awong J., Bitton G., and Koopman B. ATP, oxygen-uptake rate and INT-dehydrogenase activity of actinomycete foams. Water Res. 19 (1985) 917-921
2. Bensaid A., Thierie J., and Penninckx M. The use of the tetrazolium salt XTT for the estimation of biological activity of activated sludge cultivated under steady state and transient regimes. J. Microbiol. Methods 40 (2000) 255-263
3. Bigalke D.L. Methods used for monitoring the microbiological quality of raw milk. Part I. Dairy Food Sanit. 4 5 (1984) 189-190
4. Dalzell D.J.B., Alte S., Aspichueta E., de la Sota A., Etxebarria J., Gutierrez M., Hoffmann C.C., Sales D., Obst U., and Christofi N. A comparison of five rapid direct toxicity assessment methods to determine toxicity of pollutants to activated sludge. Chemosphere 47 (2002) 535-545
5. de Bel M., Stokes l., Upton J., and Watts J. Applications of a respirometry based toxicity monitor. Water Sci. Technol. 33 (1996) 289-296
6. Erb R.E., and Ehlers M.H. Resazurin reducing time as an indicator of bovine semen capacity. J. Dairy Sci. 33 (1950) 853-864
7. Evans M.R., Jordinson G.M., Rawson D.M., and Rogerson J.G. Biosensors for the measurement of toxicity of wastewater to activated sludge. Pestic. Sci. 54 (1998) 447-452
8. Fung D.Y.C., and Miller R.D. Effect of dyes on bacterial growth. Appl. Microbiol. 25 (1973) 793-799
9. Guerin T.F., Mondido M., McClenn B., and Peasley B. Application of resazurin for estimating abundance of contaminant-degrading microorganisms. Lett. Appl. Microbiol. 32 (2001) 340-345
10. Jo K.H., and Silverstein J. Acclimation of activated sludge to degrade toxic levels of 2,4-dinitrophenol. Water Sci. Technol. 50 (2004) 45-50
11. Kim C.W., Koopman B., and Bitton G. INT-dehydrogenase activity test for assessing chlorine and hydrogen peroxide inhibition of filamentous pure cultures and activated sludge. Water Res. 28 (1994) 1117-1121
12. Liu D., and Strachan W.M.J. Characterization of microbial activity in sediment by resazurin reduction. Arch. Hydrobiol. Beih. 12 (1979) 24-31
13. Liu D. A rapid biochemical test for measuring chemical toxicity. Bull. Environ. Contam. Toxicol. 26 (1981) 145-149
14. Liu D. Resazurin reduction method for activated sludge process control. Environ. Sci. Technol. 17 (1983) 407-411
15. Liu D. Resazurin reduction method for toxicity assessment of water soluble and insoluble chemicals. Toxicity Assess.: Int. Q. 1 2 (1986) 253-258
16. McCluskey C., Quinn J.P., and McGrath J.W. An evaluation of three new-generation tetrazolium salts for the measurement of respiratory activity in activated sludge microorganisms. Microb. Ecol. 49 (2005) 379-387
17. Nirmalakhandan N., Peace J., Egemen E., Mohsin M., Sun B., and Hall E. Estimating toxicity of mixtures of organic chemicals to activated sludge using surrogate test cultures. Water Sci. Technol. 34 (1996) 87-92
18. O'Brien J., Wilson I., Orton T., and Pognan F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur. J. Biochem. 267 (2000) 5421-5426
19. Packard T.T. Measurement of electron transport activity of microplankton. In: Jannasch H.W., and Williams P.J. (Eds). Advances in Aquatic Microbiology (1985), Academic Press Inc. Ltd, London 207-262
20. Rasmussen E.S. Use of fluorescent redox indicators to evaluate cell proliferation and viability. In Vitro Mol. Toxicol. 12 (1999) 47-58
21. Roslev P., and King G.M. Application of a tetrazolium salt with a water-soluble formazan as an indicator of viability in respiring bacteria. Appl. Environ. Microbiol. 59 (1993) 2891-2896
22. Strotmann U.J., Butz B., and Bias W.R. A dehydrogenase assay with resazurin-practical performance as a monitoring-system and pH-dependent toxicity of phenolic-compounds. Ecotoxic Environ. Safe 25 (1993) 372
23. Wang J.L. Effect of di-n-butyl phthalate (DBP) on activated sludge. Process Biochem. 39 (2004) 1831-1836