Activity-enhancing mutations in an E3 ubiquitin ligase identified by high-throughput mutagenesis

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 14, 2013, Pages

  Starita, Lea M ^a   Pruneda, Jonathan N ^b   Lo, Russell S ^a   Fowler, Douglas M ^a   Kim, Helen J ^a   Hiatt, Joseph B ^a   Shendure, Jay ^a   Brzovic, Peter S ^b   Fields, Stanley ^a   Klevit, Rachel E ^b  

^a UNIVERSITY OF WASHINGTON   (United States)

^b UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

Deep mutational scanning; NMR; Phage display; Protein stability; Ubiquitin E3 ligase

Indexed keywords

PROTEIN P53; PROTEIN VARIANT; UBIQUITIN PROTEIN LIGASE; UBIQUITINATION FACTOR E4B; UNCLASSIFIED DRUG;

ALLOSTERISM; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; GENE MUTATION; MOLECULAR MECHANICS; MOUSE; MUTAGENESIS; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; UBIQUITINATION;

ALLOSTERIC REGULATION; AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CELL SURFACE DISPLAY TECHNIQUES; HIGH-THROUGHPUT SCREENING ASSAYS; IMMUNOPRECIPITATION; MICE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; OLIGONUCLEOTIDES; SEQUENCE ALIGNMENT; UBIQUITIN; UBIQUITIN-PROTEIN LIGASES;

MURINAE;

EID: 84875870975     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1303309110     Document Type: Article

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