Silence of ezrin modifies migration and actin cytoskeleton rearrangements and enhances chemosensitivity of lung cancer cells in vitro

Molecular and Cellular Biochemistry

Volumn 377, Issue 1-2, 2013, Pages 207-218

  Chen, Qing Yong ^a   Xu, Wei ^b   Jiao, De Min ^a   Wu, Li Jun ^a   Song, Jia ^a   Yan, Jie ^a   Shi, Jian Guo ^a  

^a The 117th Hospital of Chinese PLA   (China)

^b ZHEJIANG CHINESE MEDICAL UNIVERSITY   (China)

Author keywords

Actin cytoskeleton; Chemosensitivity; Ezrin; Lung cancer cells; Migration and invasion; RNA interference

Indexed keywords

CD44V ANTIGEN; CHOLECYSTOKININ OCTAPEPTIDE; CISPLATIN; DOCETAXEL; EZRIN; GEMCITABINE; PIRARUBICIN; SHORT HAIRPIN RNA; UVOMORULIN;

ACTIN FILAMENT; APOPTOSIS; ARTICLE; CANCER CELL; CANCER INHIBITION; CARCINOGENICITY; CELL CYCLE; CELL FUNCTION; CELL GROWTH; CELL INVASION; CELL MIGRATION; CELL MOTILITY; CELL PROLIFERATION; CHEMOSENSITIVITY; CONFOCAL MICROSCOPY; DOWN REGULATION; FLOW CYTOMETRY; FOCAL ADHESION; GENE EXPRESSION; GENE SILENCING; HUMAN; HUMAN CELL; LUNG CANCER; METASTASIS; NUCLEOTIDE SEQUENCE; PSEUDOPODIUM; STRESS FIBER; TUMOR GROWTH; WESTERN BLOTTING; WHOLE CELL;

ACTIN CYTOSKELETON; ANTIGENS, CD44; ANTINEOPLASTIC AGENTS; APOPTOSIS; CADHERINS; CELL CYCLE; CELL LINE, TUMOR; CELL MOVEMENT; CELL PROLIFERATION; CISPLATIN; CYTOSKELETAL PROTEINS; DRUG RESISTANCE, NEOPLASM; GENE EXPRESSION; GENE KNOCKDOWN TECHNIQUES; HUMANS; RNA, SMALL INTERFERING;

LENTIVIRUS;

EID: 84875671343     PISSN: 03008177     EISSN: 15734919     Source Type: Journal    
DOI: 10.1007/s11010-013-1586-x     Document Type: Article

References (30)

1. Nesbitt JC, Putnam JB Jr, Walsh GL, Roth JA, Mountain CF (1995) Survival in early-stage non-small cell lung cancer. Ann Thorac Surg 60:466-472
2. Makitie T, Carpen O, Vaheri A, Kivela T (2001) Ezrin as a prognostic indicator and its relationship to tumor characteristics in uveal malignant melanoma. Invest Ophthalmol Vis Sci 42:2442-2449
3. Ohtani K, Sakamoto H, Rutherford T, Chen Z, Satoh K, Naftolin F (1999) Ezrin, a membrane-cytoskeletal linking protein, is involved in the process of invasion of endometrial cancer cells. Cancer Lett 147:31-38
4. Geiger KD, Stoldt P, Schlote W, Derouiche A (2000) Ezrin immunoreactivity is associated with increasing malignancy of astrocytic tumors but is absent in oligodendrogliomas. Am J Pathol 157:1785-1793. doi: 10.1016/S0002-9440(10)64816- X
5. Kang YK, Hong SW, Lee H, Kim WH (2010) Prognostic implications of ezrin expression in human hepatocellular carcinoma. Mol Carcinog 49:798-804. doi: 10.1002/mc.20653
6. Meng Y, Lu Z, Yu S, Zhang Q, Ma Y, Chen J (2010) Ezrin promotes invasion and metastasis of pancreatic cancer cells. J Transl Med 8:61. doi: 10.1186/1479-5876-8-61
7. Moilanen J, Lassus H, Leminen A, Vaheri A, Butzow R, Carpen O (2003) Ezrin immunoreactivity in relation to survival in serous ovarian carcinoma patients. Gynecol Oncol 90:273-281
8. Tokunou M, Niki T, Saitoh Y, Imamura H, Sakamoto M, Hirohashi S (2000) Altered expression of the ERM proteins in lung adenocarcinoma. Lab Invest 80:1643-1650
9. Chen QY, Yan J, Hu HZ, Chen FY, Song J, Jiang ZY, Jiao DM, Wu YQ (2012) Expression of ezrin in human non-small cell lung cancer and its relationship with metastasis and prognosis. Zhonghua Zhong Liu Za Zhi 34:436-440
10. Khanna C, Khan J, Nguyen P, Prehn J, Caylor J, Yeung C, Trepel J, Meltzer P, Helman L (2001) Metastasis-associated differences in gene expression in a murine model of osteosarcoma. Cancer Res 61:3750-3759
11. Li Q, Wu M, Wang H, Xu G, Zhu T, Zhang Y, Liu P, Song A, Gang C, Han Z, Zhou J, Meng L, Lu Y, Wang S, Ma D (2008) Ezrin silencing by small hairpin RNA reverses metastatic behaviors of human breast cancer cells. Cancer Lett 261:55-63. doi: 10.1016/j.canlet.2007.11.018
12. Martin TA, Harrison G, Mansel RE, Jiang WG (2003) The role of the CD44/ezrin complex in cancer metastasis. Crit Rev Oncol Hematol 46:165-186
13. Pujuguet P, Del Maestro L, Gautreau A, Louvard D, Arpin M (2003) Ezrin regulates E-cadherin-dependent adherens junction assembly through Rac1 activation. Mol Biol Cell 14:2181-2191. doi: 10.1091/mbc.E02-07-0410
14. Kim SY, Jung SH, Kim HS (2005) Curcumin is a potent broad spectrum inhibitor of matrix metalloproteinase gene expression in human astroglioma cells. Biochem Biophys Res Commun 337:510-516. doi: 10.1016/j.bbrc.2005.09.079
15. Ji C, Cao C, Lu S, Kivlin R, Amaral A, Kouttab N, Yang H, Chu W, Bi Z, Di W, Wan Y (2008) Curcumin attenuates EGF-induced AQP3 up-regulation and cell migration in human ovarian cancer cells. Cancer Chemother Pharmacol 62:857-865. doi: 10.1007/s00280-007-0674-6
16. Chen QY, Jiao DM, Yao QH, Yan J, Song J, Chen FY, Lu GH, Zhou JY (2012) Expression analysis of Cdc42 in lung cancer and modulation of its expression by curcumin in lung cancer cell lines. Int J Oncol 40:1561-1568. doi: 10.3892/ijo.2012.1336
17. Han Z, Xu G, Zhou J, Xing H, Wang S, Wu M, Zhang Y, Lu Y, Ma D (2005) Inhibition of motile and invasive properties of ovarian cancer cells by ASODN against Rho-associated protein kinase. Cell Commun Adhes 12:59-69. doi: 10.1080/15419060500383168
18. Underhill C (1992) CD44: the hyaluronan receptor. J Cell Sci 103(Pt 2):293-298
19. Yonemura S, Hirao M, Doi Y, Takahashi N, Kondo T, Tsukita S (1998) Ezrin/radixin/moesin (ERM) proteins bind to a positively charged amino acid cluster in the juxta-membrane cytoplasmic domain of CD44, CD43, and ICAM-2. J Cell Biol 140:885-895
20. Khanna C, Wan X, Bose S, Cassaday R, Olomu O, Mendoza A, Yeung C, Gorlick R, Hewitt SM, Helman LJ (2004) The membrane-cytoskeleton linker ezrin is necessary for osteosarcoma metastasis. Nat Med 10:182-186. doi: 10.1038/nm982
21. Zhang Y, Hu MY, Wu WZ, Wang ZJ, Zhou K, Zha XL, Liu KD (2006) The membrane-cytoskeleton organizer ezrin is necessary for hepatocellular carcinoma cell growth and invasiveness. J Cancer Res Clin Oncol 132:685-697. doi: 10.1007/s00432-006-0117-5
22. Akisawa N, Nishimori I, Iwamura T, Onishi S, Hollingsworth MA (1999) High levels of ezrin expressed by human pancreatic adenocarcinoma cell lines with high metastatic potential. Biochem Biophys Res Commun 258:395-400. doi: 10.1006/bbrc.1999.0653
23. Yu Y, Khan J, Khanna C, Helman L, Meltzer PS, Merlino G (2004) Expression profiling identifies the cytoskeletal organizer ezrin and the developmental homeoprotein Six-1 as key metastatic regulators. Nat Med 10:175-181. doi: 10.1038/nm966
24. Horita Y, Ohashi K, Mukai M, Inoue M, Mizuno K (2008) Suppression of the invasive capacity of rat ascites hepatoma cells by knockdown of Slingshot or LIM kinase. J Biol Chem 283:6013-6021. doi: 10.1074/jbc.M706538200
25. Turunen O, Wahlstrom T, Vaheri A (1994) Ezrin has a COOH-terminal actin-binding site that is conserved in the ezrin protein family. J Cell Biol 126:1445-1453
26. Park HR, Jung WW, Bacchini P, Bertoni F, Kim YW, Park YK (2006) Ezrin in osteosarcoma: comparison between conventional high-grade and central low-grade osteosarcoma. Pathol Res Pract 202:509-515. doi: 10.1016/j.prp.2006.01.015
27. Hunter KW (2004) Ezrin, a key component in tumor metastasis. Trends Mol Med 10:201-204. doi: 10.1016/j.molmed.2004.03.001
28. Shang X, Wang Y, Zhao Q, Wu K, Li X, Ji X, He R, Zhang W (2012) siRNAs target sites selection of ezrin and the influence of RNA interference on ezrin expression and biological characters of osteosarcoma cells. Mol Cell Biochem 364:363-371. doi: 10.1007/s11010-012-1238-6
29. Yang MH, Zhao MY, Wang Z, Kang R, He YL, Yin XC, Liu LY, Yang LC, Zhan CX, Wu XS, Tang DL, Cao LZ (2011) WAVE1 regulates P-glycoprotein expression via ezrin in leukemia cells. Leuk Lymphoma 52:298-309. doi: 10.3109/10428194.2010. 538776
30. Kano T, Wada S, Morimoto K, Kato Y, Ogihara T (2011) Effect of knockdown of ezrin, radixin, and moesin on P-glycoprotein function in HepG2 cells. J Pharm Sci 100:5308-5314. doi: 10.1002/jps.22718