Monoclonal antibody heterogeneity analysis and deamidation monitoring with high-performance cation-exchange chromatofocusing using simple, two component buffer systems

Journal of Chromatography A

Volumn 1283, Issue , 2013, Pages 89-97

  Kang, Xuezhen ^a   Kutzko, Joseph P ^a   Hayes, Michael L ^a   Frey, Douglas D ^b  

^a SANOFI   (France)

^b University of Marylandw Baltimore County   (United States)

Author keywords

Charge variants; Chromatofocusing; Isoelectric point; Monoclonal antibody

Indexed keywords

ACID-BASE EQUILIBRIA; CATION EXCHANGES; CHARGE VARIANTS; CHROMATOFOCUSING; HIGH RESOLUTION; ISO-ELECTRIC POINTS; ISOELECTRIC FOCUSING; SPECIES CONCENTRATION;

CHEMICAL REACTIONS; CHROMATOGRAPHY; ELECTROPHORESIS; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; POSITIVE IONS; SURFACE PLASMON RESONANCE;

PH;

AMPHOLYTE; BUFFER; CITRIC ACID; DIETHANOLAMINE; ETHANOLAMINE; MONOCLONAL ANTIBODY; POLYAMPHOLYTE; TRIETHANOLAMINE; UNCLASSIFIED DRUG;

ACIDITY; ADSORPTION; ALKALINITY; ARTICLE; CHROMATOFOCUSING; COMPUTER SIMULATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DEAMINATION; DRUG STABILITY; HIGH PERFORMANCE CATION EXCHANGE CHROMATOFOCUSING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOELECTRIC FOCUSING; ISOELECTRIC POINT; MASS SPECTROMETRY; MOLECULAR SIZE; PH; PRIORITY JOURNAL; PURIFICATION; SURFACE CHARGE;

ANTIBODIES, MONOCLONAL; BUFFERS; CATIONS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; CITRIC ACID; COMPUTER SIMULATION; HYDROGEN-ION CONCENTRATION; ISOELECTRIC POINT; PROTEIN ISOFORMS; PROTEIN STABILITY; SODIUM HYDROXIDE;

EID: 84875371804     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2013.01.101     Document Type: Article

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