Comparison of whole blood and PBMC assays for T-cell functional analysis

BMC Research Notes

Volumn 6, Issue 1, 2013, Pages

  Deenadayalan, Anbarasu ^a   Maddineni, Prabhavathi ^a   Raja, Alamelu ^a  

^a NATIONAL INSTITUTE FOR RESEARCH IN TUBERCULOSIS   (India)

Author keywords

Interferon ; Peripheral blood mononuclear cells; T lymphocytes; Tuberculosis; Whole blood assays

Indexed keywords

MYCOBACTERIUM TUBERCULOSIS;

ANTIGEN; BACTERIAL ANTIGEN; GAMMA INTERFERON; RECOMBINANT PROTEIN;

ADULT; BLOOD; CELL PROLIFERATION; COMPARATIVE STUDY; CYTOLOGY; DIAGNOSTIC TEST; HUMAN; IMMUNOLOGY; METABOLISM; MONONUCLEAR CELL; MYCOBACTERIUM TUBERCULOSIS; PROCEDURES; T LYMPHOCYTE; TUBERCULOSIS; ARTICLE; METHODOLOGY;

ADULT; ANTIGENS; ANTIGENS, BACTERIAL; CELL PROLIFERATION; DIAGNOSTIC TESTS, ROUTINE; HUMANS; INTERFERON-GAMMA; LEUKOCYTES, MONONUCLEAR; MYCOBACTERIUM TUBERCULOSIS; RECOMBINANT PROTEINS; T-LYMPHOCYTES; TUBERCULOSIS;

EID: 84875351094     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/1756-0500-6-120     Document Type: Article

References (13)

1. World Health Organization, Global Tuberculosis Control-Surveillance, Planning, Financing, WHO Report Geneva: World Health Organization 2011 www.who.int/tb/publications/global-report/2011/gtbr11-full.pdf 23537342
2. Transfer of adoptive immunity to tuberculosis in mice. Lefford MJ, Infect Immun 1975 11 1174 1181 806520
3. Immunology of tuberculosis and implications in vaccine development. Flynn JL, Tuberculosis (Edinb) 2004 84 93 101 10.1016/j.tube.2003.08.010
4. An essential role for interferon gamma in resistance to Mycobacterium tuberculosis infection. Flynn JL, Chan J, Triebold KJ, Dalton DK, Stewart TA, J Exp Med 1993 178 2249 2254 10.1084/jem.178.6.2249 7504064
5. Novel human immunodeficiencies reveal the essential role of type-I cytokines in immunity to intracellular bacteria. Ottenhoff TH, Kumararatne D, Casanova JL, Immunol Today 1998 19 491 494 10.1016/S0167-5699(98)01321-8 9818540
6. Correlation between the kinetics of Thl/Th2 cells and pathology in a murine model of experimental pulmonary tuberculosis. Hernandez-Pando R, Orozcoe H, Sampieri A, Pavon L, Immunology 1996 89 26 33 8911136
7. Disseminated tuberculosis in interferon gamma gene-disrupted mice. Cooper AM, Dalton DK, Stewart TA, Griffin JP, Russell DG, J Exp Med 1993 178 2243 2247 10.1084/jem.178.6.2243 8245795
8. Immunological outcomes of new tuberculosis vaccine trials: WHO panel recommendations. Hanekom WA, Dockrell HM, Ottenhoff TH, Doherty TM, Fletcher H, PLoS Med 2008 5 145 10.1371/journal.pmed.0050145 18597551
9. Cloning, expression, and purification of the 27 kDa (MPT51, Rv3803c) protein of Mycobacterium tuberculosis. Ramalingam B, Baulard AR, Locht C, Narayanan PR, Raja A, Protein Expr Purif 2004 36 53 60 10.1016/j.pep.2004.01.016 15177284
10. Discrepancy between mycobacterium tuberculosis-specific gamma interferon release assays using short and prolonged in vitro incubation. Eliane M, Leyten S, Sandra M, Arend, Corine Prins, Clin Vaccine Immunol 2007 14 7 880 885 10.1128/CVI.00132-07 17507543
11. Whole blood assay to access T cell-immune responses to mycobacterium tuberculosis antigens in healthy Brazilian individuals. Cruz MIO, de Janeiro R, Immunology and immunotherapy 2004 99 1 53 55
12. Comparison of cytokine production in cultures of whole human blood and purified mononuclear cells. Yaqoob P, Newsholme EA, Calder PC, Cytokine 1999 11 8 600 605 10.1006/cyto.1998.0471 10433807
13. Immunoproteomic identification of human T cell antigens of mycobacterium tuberculosis that differentiate healthy contacts from tuberculosis patients. Deenadayalan A, Aavudaiyappan Rajendiran A, Vaithilingam Velayudham B, Frederick S, Yang HL, Dobos K, John T, Belisle, Raja A, Mol Cell Proteomics 2009 9 538 549 20031926