Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments

BMC Biotechnology

Volumn 13, Issue , 2013, Pages

  Ohmuro Matsuyama, Yuki ^a,b,c   Chung, Chan I ^a   Ueda, Hiroshi ^a,c  

^a UNIVERSITY OF TOKYO   (Japan)

^b JAPAN SOCIETY FOR THE PROMOTION OF SCIENCE   (Japan)

^c TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Bioluminescence; Firefly luciferase; In vitro diagnostics; Protein fragment complementation assay; Protein protein interaction; Thermostability

Indexed keywords

FIREFLY LUCIFERASE; IN-VITRO DIAGNOSTIC; PROTEIN FRAGMENT COMPLEMENTATION ASSAYS; PROTEIN-PROTEIN INTERACTIONS; THERMOSTABILITY;

ASSAYS; BIOLUMINESCENCE; ENERGY TRANSFER; ESCHERICHIA COLI; PROBES; PURIFICATION; SIGNAL DETECTION;

PROTEINS;

FIREFLY LUCIFERASE; GREEN FLUORESCENT PROTEIN; PROTEIN MDM2; PROTEIN P53; RAPAMYCIN;

ARTICLE; BIOASSAY; ESCHERICHIA COLI; FLUORESCENCE RESONANCE ENERGY TRANSFER; MOLECULAR PROBE; PROTEIN EXPRESSION; PROTEIN FRAGMENT COMPLEMENTATION ASSAY; PROTEIN PROTEIN INTERACTION; THERMOSTABILITY;

ANIMALS; ESCHERICHIA COLI; FIREFLIES; FLUORESCENCE RESONANCE ENERGY TRANSFER; LUCIFERASES, FIREFLY; PROTEIN INTERACTION MAPPING; PROTEIN INTERACTION MAPS; PROTO-ONCOGENE PROTEINS C-MDM2; RECOMBINANT FUSION PROTEINS; SIGNAL-TO-NOISE RATIO; SIROLIMUS; TACROLIMUS BINDING PROTEIN 1A; TUMOR SUPPRESSOR PROTEIN P53;

EID: 84875291004     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-13-31     Document Type: Article

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