Nested culture method improves detection of fusobacterium from stool in patients with ulcerative colitis

Japanese Journal of Infectious Diseases

Volumn 66, Issue 2, 2013, Pages 109-114

  Yukawa, Toyokazu ^a   Ohkusa, Toshifumi ^a   Shibuya, Tomoyoshi ^b   Tsukinaga, Shintaro ^a   Mitobe, Jimi ^a   Takakura, Kazuki ^a   Takahara, Akitaka ^a   Odahara, Shunichi ^a   Matsudaira, Hiroshi ^a   Nagatsuma, Keisuke ^a   Kitahara, Takuya ^a   Kajihara, Mikio ^a   Uchiyama, Kan ^a   Arakawa, Hiroshi ^a   Koido, Shigeo ^a   Tajiri, Hisao ^c  

^a JIKEI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b JUNTENDO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^c JIKEI UNIVERSITY HOSPITAL   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMOXICILLIN; CRYSTAL VIOLET; GLUCOCORTICOID; IMMUNOSUPPRESSIVE AGENT; MESALAZINE; METRONIDAZOLE; NEOMYCIN; PROBIOTIC AGENT; SALAZOSULFAPYRIDINE; TETRACYCLINE;

ADULT; AGED; ANTIBIOTIC THERAPY; ARTICLE; BACTERIUM COLONY; BACTERIUM CULTURE; BACTERIUM ISOLATION; CLINICAL ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE TECHNIQUE; DISEASE ASSOCIATION; FECES ANALYSIS; FECES CULTURE; FEMALE; FUSOBACTERIUM VARIUM; HUMAN; IMMUNOHISTOCHEMISTRY; MALE; NESTED CULTURE METHOD; PATIENT CODING; POLYMERASE CHAIN REACTION; PROSPECTIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; SEROLOGY; TREATMENT DURATION; ULCERATIVE COLITIS;

ADULT; AGED; BACTERIAL TYPING TECHNIQUES; BACTERIOLOGICAL TECHNIQUES; COLITIS, ULCERATIVE; CULTURE MEDIA; FECES; FEMALE; FUSOBACTERIUM; HUMANS; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; PROSPECTIVE STUDIES; YOUNG ADULT;

EID: 84875280786     PISSN: 13446304     EISSN: 18842836     Source Type: Journal    
DOI: 10.7883/yoken.66.109     Document Type: Article

References (19)

1. Ohkusa, T., Sato, N., Ogihara, T., et al. (2002): Fusobacterium varium localized in the colonic mucosa of patients with ulcerative colitis stimulates species-specific antibody. J. Gastroenterol. Hepatol., 17, 849-853.
2. Ohkusa, T., Okayasu, I., Ogihara, T., et al. (2003): Induction of experimental ulcerative colitis by Fusobacterium varium isolated from colonic mucosa of patients with ulcerative colitis. Gut, 52, 79-83.
3. Yoshida, T., Haga, S., Numata, Y., et al. (2006): Disruption of p53-p53R2 DNA repair system in ulcerative colitis contributes to colon tumorigenesis. Int. J. Cancer, 118, 1395-1403.
4. Ohkusa, T., Nomura, T., Terai, T., et al. (2005): Effectiveness of antibiotic combination therapy in patients with active ulcerative colitis: a randomized, controlled pilot trial with long-term followup. Scand. J. Gastroenterol., 40, 1334-1342.
5. Ohkusa, T., Kato, K., Terao, S., et al. (2010): Newly developed antibiotic combination therapy for ulcerative colitis: a doubleblind placebo-controlled multicenter trial. Am. J. Gastroenterol., 105, 1820-1829.
6. Nomura, T., Ohkusa, T., Okayasu, I., et al. (2005): Mucosaassociated bacteria in ulcerative colitis before and after antibiotic combination therapy. Aliment. Pharmacol. Ther., 21, 1017-1027.
7. Strauss, J., Kaplan, G.G., Beck, P.L., et al. (2011): Invasive potential of gut mucosa-derived Fusobacterium nucleatum positively correlates with IBD status of the host. Inflamm. Bowel Dis., 17, 1971-1978.
8. Lichtiger, S., Present, D.H., Kornbluth, A., et al. (1994): Cyclosporine in severe ulcerative colitis refractory to steroid therapy. N. Engl. J. Med., 330, 1841-1845.
9. Strauss, J., White, A., Ambrose, C., et al. (2008) Phenotypic and genotypic analyses of clinical Fusobacterium nucleatum and Fusobacterium periodonticum isolates from the human gut. Anaerobe, 14, 301-309.
10. Sartor, R.B. (2008): Microbial influences in inflammatory bowel diseases. Gastroenterology, 134, 577-594.
11. Ogura, Y., Bonen, D.K., Inohara, N., et al. (2001): A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease. Nature, 411, 603-606.
12. Agnese, D.M., Calvano, J.E., Hahm, S.J., et al. (2002): Human toll-like receptor 4 mutations but not CD14 polymorphisms are associated with an increased risk of gram-negative infections. J. Infect. Dis., 186, 1522-1525.
13. Darfeuille-Michaud, A., Neut, C., Barnich, N., et al. (1998): Presence of adherent Escherichia coli strains in ileal mucosa of patients with Crohn's disease. Gastroenterology, 115, 1405-1413.
14. Petersen, A.M., Nielsen, E.M., Litrup, E., et al. (2009): A phylogenetic group of Escherichia coli associated with active leftsided inflammatory bowel disease. BMC Microbiol., 9, 171.
15. Sepehri, S., Kotlowski, R., Bernstein, C.N., et al. (2007): Microbial diversity of inflamed and noninflamed gut biopsy tissues in inflammatory bowel disease. Inflamm. Bowel Dis., 13, 675-683.
16. Frank, D.N., St Amand, A.L., Feldman, R.A., et al. (2007): Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases. Proc. Natl. Acad. Sci. USA, 104, 13780-13785.
17. Odamaki, T., Xiao, J.Z., Sakamoto, M., et al. (2008): Distribution of different species of the Bacteroides fragilis group in individuals with Japanese cedar pollinosis. Appl. Environ. Microbiol., 74, 6814-6817.
18. Minami, M., Ando, T., Okamoto, A., et al. (2009): Seroprevalence of Fusobacterium varium in ulcerative colitis patients in Japan. FEMS Immunol. Med. Microbiol., 56, 67-72.
19. Swidsinski, A, Dorffel, Y., Loening-Baucke, V., et al. (2011): Acute appendicitis is characterized by local invasion with Fusobacterium nucleatum/necrophorum. Gut, 60, 34-40.