A flow cytometry based assay for the enumeration of regulatory T cells in whole blood

Journal of Immunological Methods

Volumn 390, Issue 1-2, 2013, Pages 121-126

  Hardy, Melinda Y ^a   Vari, Frank ^a,b   Rossetti, Tony ^a   Hart, Derek N ^a,c   Prue, Rebecca L ^a  

^a MATER MEDICAL RESEARCH INSTITUTE   (Australia)

^b QIMR BERGHOFER MEDICAL RESEARCH INSTITUTE   (Australia)

^c Department of Microbiology and Infectious Diseases   (Australia)

Author keywords

Regulatory T cells; TruCOUNT ; Whole blood

Indexed keywords

INTERLEUKIN 7 RECEPTOR; TRANSCRIPTION FACTOR FOXP3;

ANTIGEN EXPRESSION; ARTICLE; BLOOD; CD4+ CD25+ T LYMPHOCYTE; CD4+ T LYMPHOCYTE; CELL ASSAY; CELL COUNT; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MEASUREMENT PRECISION; NORMAL HUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; REGULATORY T LYMPHOCYTE; RELIABILITY; REPRODUCIBILITY;

BLOOD SPECIMEN COLLECTION; FLOW CYTOMETRY; FORKHEAD TRANSCRIPTION FACTORS; HUMANS; INTERLEUKIN-2 RECEPTOR ALPHA SUBUNIT; INTERLEUKIN-7 RECEPTOR ALPHA SUBUNIT; LEUKOCYTES, MONONUCLEAR; LYMPHOCYTE COUNT; REPRODUCIBILITY OF RESULTS; T-LYMPHOCYTES, REGULATORY;

EID: 84875268821     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2012.07.004     Document Type: Article

References (18)

1. + T-regulatory cells: toward therapy for human diseases. Immunol. Rev. 2008, 223:391.
2. Bremm M., Huenecke S., Lehrnbecher T., Ponstingl E., Mueller R., Heinze A., Bug G., Quaiser A., Kapinsky M., Brehm C., Bader P., Schneider G., Klingebiel T., Koehl U. Advanced flowcytometric analysis of regulatory T cells: CD127 downregulation early post stem cell transplantation and altered Treg/CD3(+)CD4(+)-ratio in severe GvHD or relapse. J. Immunol. Methods 2011, 373:36.
3. Brocklebank A.M., Sparrow R.L. Enumeration of CD34+ cells in cord blood: a variation on a single-platform flow cytometric method based on the ISHAGE gating strategy. Cytometry 2001, 46:254.
4. Fazekas de St Groth B., Zhu E., Asad S., Lee L. Flow cytometric detection of human regulatory T cells. Methods Mol. Biol. 2011, 707:263.
5. Fecci P.E., Mitchell D.A., Whitesides J.F., Xie W., Friedman A.H., Archer G.E., Herndon J.E., Bigner D.D., Dranoff G., Sampson J.H. Increased regulatory T-cell fraction amidst a diminished CD4 compartment explains cellular immune defects in patients with malignant glioma. Cancer Res. 2006, 66:3294.
6. + regulatory T cells. Nat. Immunol. 2003, 4:330.
7. + regulatory T cells express lower levels of the IL-7 receptor alpha chain (CD127), allowing consistent identification and sorting of live cells. J. Immunol. Methods 2007, 319:41.
8. Hori S., Nomura T., Sakaguchi S. Control of regulatory T cell development by the transcription factor Foxp3. Science 2003, 299:1057.
9. Lin Y., Gustafson M.P., Bulur P.A., Gastineau D.A., Witzig T.E., Dietz A.B. Immunosuppressive CD14+HLA-DR(low)/- monocytes in B-cell non-Hodgkin lymphoma. Blood 2011, 117:872.
10. + T reg cells. J. Exp. Med. 2006, 203:1701.
11. Moniuszko M., Bodzenta-Lukaszyk A., Dabrowska M. Effects of oral glucocorticoid therapy on CD4+CD25+CD127- and CD4+CD25high T cell levels in asthmatic patients. Inflammation 2010, 33:415.
12. Piper K.P., Karanth M., McLarnon A., Kalk E., Khan N., Murray J., Pratt G., Moss P.A. Chronic lymphocytic leukaemia cells drive the global CD4+ T cell repertoire towards a regulatory phenotype and leads to the accumulation of CD4+ forkhead box P3+ T cells. Clin. Exp. Immunol. 2011, 166:154.
13. Seddiki N., Santner-Nanan B., Martinson J., Zaunders J., Sasson S., Landay A., Solomon M., Selby W., Alexander S.I., Nanan R., Kelleher A., de St Fazekas, Groth B. Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J. Exp. Med. 2006, 203:1693.
14. Szczepanik A.M., Siedlar M., Sierzega M., Goroszeniuk D., Bukowska-Strakova K., Czupryna A., Kulig J. T-regulatory lymphocytes in peripheral blood of gastric and colorectal cancer patients. World J. Gastroenterol. 2011, 17:343.
15. Vergati M., Cereda V., Madan R.A., Gulley J.L., Huen N.Y., Rogers C.J., Hance K.W., Arlen P.M., Schlom J., Tsang K.Y. Analysis of circulating regulatory T cells in patients with metastatic prostate cancer pre- versus post-vaccination. Cancer Immunol. Immunother. 2011, 60:197.
16. Vuckovic S., Gardiner D., Field K., Chapman G.V., Khalil D., Gill D., Marlton P., Taylor K., Wright S., Pinzon-Charry A., Pyke C.M., Rodwell R., Hockey R.L., Gleeson M., Tepes S., True D., Cotterill A., Hart D.N. Monitoring dendritic cells in clinical practice using a new whole blood single-platform TruCOUNT assay. J. Immunol. Methods 2004, 284:73.
17. - T cells. J. Clin. Invest. 2003, 112:1437.
18. + T cells. Eur. J. Immunol. 2007, 37:129.