PG-2, a potent AMP against pathogenic microbial strains, from potato (Solanum tuberosum L cv. Gogu Valley) tubers not cytotoxic against human cells

International Journal of Molecular Sciences

Volumn 14, Issue 2, 2013, Pages 4349-4360

  Kim, Jin Young ^a   Gopal, Ramamourthy ^a   Kim, Sang Young ^b   Seo, Chang Ho ^b   Lee, Hyang Burm ^c   Cheong, Hyeonsook ^a   Park, Yoonkyung ^a  

^a Chosun University   (South Korea)

^b Kongju National University   (South Korea)

^c Chonnam National University   (South Korea)

Author keywords

Antimicrobial agent; Human pathogenic; N terminal sequence; Peptide G2; RP HPLC

Indexed keywords

PEPTIDE G2; POLYPEPTIDE ANTIBIOTIC AGENT; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANTIBACTERIAL ACTIVITY; ANTIFUNGAL ACTIVITY; ANTIMICROBIAL ACTIVITY; ARTICLE; BACTERIUM; CANDIDA ALBICANS; CLAVIBACTER MICHIGANENSIS; CONTROLLED STUDY; DRUG CYTOTOXICITY; DRUG DETERMINATION; DRUG SCREENING; HEMOLYSIS; HUMAN; HUMAN CELL; MOLECULAR WEIGHT; NONHUMAN; POTATO; STAPHYLOCOCCUS AUREUS; THERMOSTABILITY; TOXICITY TESTING;

EID: 84875115374     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms14024349     Document Type: Article

References (24)

1. Osborn, W.F.; Broekaert, F.R.; Terras, B.P.; Cammue, R.W. Plant defensins: Novel antimicrobial peptides as components of the host defense system. Plant Physiol. 1995, 108, 1353-1358.
2. Hultmark, H.G.; Boman, D. Cell-free immunity in insects. Annu. Rev. Microbiol. 1987, 41, 103-126.
3. Hoffmann, J.A. Innate immunity of insects. Curr. Opin. Immunol. 1995, 7, 4-10.
4. Park, J.-K.; Lee, R.; Gopal, C.H.; Seo, H.; Cheong, Y. Isolation and Purification of a Novel Deca-Antifungal Peptide from Potato (Solanum tuberosum L cv. Jopung) Against Candida albicans. Int. J. Mol. Sci. 2012, 13, 4021-4032.
5. Simmaco, D.; Barra, M. Amphibian skin: A promising resource for antimicrbial peptides. Trends Biotechnol. 1995, 13, 205-209.
6. Ganz, R.I.; Lehrer, A.K.; Lichtenstein, T. Defensins: Antimicrobial and cytotoxic peptides of mammalian cells. Annu. Rev. Immunol. 1993, 1, 105-128.
7. Sitaram, C.; Subbalakshmi, N. Mechanism of antimicrobial action of indolicidin. FEMS Microbiol. Lett. 1998, 16, 91-96.
8. Glazebrook, J. Genes controlling expression of defense responses in Arabidopsis. Curr. Opin. Plant Biol. 1999, 2, 280-286.
9. Hahm, J.-Y.; Kim, S.Y.; Lee, S.-C.; Park, S.Y.; Shin, S.J.; Choi, Y.; Park, K.-S. Purification and antimicrobial activity studies of the N-terminal fragment of ubiquitin from human amniotic fluid. Biochim. Biophys. Acta 2007, 1774, 1221-1226.
10. Garcia-Olmedo, A.; Segura, M.; Moreno, F.; Madueno, A.; Molina, F. Snakin-1, a peptide from potato that is active against plant pathogens. Mol. Plant Microb Interact. 1999, 12, 16-23.
11. Molina, M.; Berrocal-Lobo, A.; Segura, M.; Moreno, G.; Lopez, F.; Garcia-Olmedo, A. Snakin-2, an antimicrobial peptide from potato whose gene is locally induced by wounding and responds to pathogen infection. Plant Physiol. 2002, 128, 951-961.
12. Park, M.H.; Kim, S.C.; Park, J.Y.; Kim, S.Y.; Lee, H.T.; Lim, H.; Cheong, K.S.; Hahm, Y. Purification and characterization of a heat-stable serine protease inhibitor from the tubers of new potato variety "Golden Valley". Biochem. Biophys. Res. Commun. 2006, 346, 681-686.
13. Hahm, J.Y.; Kim, S.C.; Park, M.H.; Kim, H.T.; Lim, Y.; Park, K.S. Antimicrobial activity studies on a trypsin-chymotrypsin protease inhibitor obtained from potato. Biochem. Biophys. Res. Commun. 2005, 330, 921-927.
14. Van Strien, L.C.; van Loon, E.A. The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins. Physiol. Mol. Plant Pathol. 1999, 55, 85-97.
15. Garg, V.R.; Tripathi, S.; Kumar, S.K. A study on trypsin, Aspergillus flavus and Bacillus sp. protease inhibitory activity in Cassia tora (L.) syn Senna tora (L.) Roxb. seed extract. BMC Complement Altern. Med. 2011, 11, 56.
16. Tian, S.B.; Liu, X.C.; Wang, M.J.; Shi, Y.Y.; Chen, Z.H.; Hu, W.M. Vegetative storage protein with trypsin inhibitor activity occurs in Sapindus mukorassi, a sapindaceae deciduous tree. J. Integr. Plant Biol. 2009, 51, 352-359.
17. Sano, Y.K.; Yap, Y.; Kodama, F.; Waller, K.M.; Chung, H.; Ueda, K.; Nakamura, M.; Oldsen, H.; Yoda, Y.; Yamaguchi, H. Activation of a novel transcription factor through phosphorylation by WIPK, a wound-induced mitogen-activated protein kinase in tobacco plants. Plant Physiol. 2005, 139, 127-137.
18. Wu, M.; Heinrich, I.T.; Baldwin, J. Two mitogen-activated protein kinase kinases, MKK1 and MEK2, are involved in wounding-and specialist lepidopteran herbivore Manduca sexta-induced responses in Nicotiana attenuata. J. Exp. Bot. 2011, 62, 4355-4365.
19. Domoney, A.; Clemente, M.; Carmen Marín-Manzano, E.; Jiménez, M.; Carmen Arques, C. The anti-proliferative effect of TI1B, a major Bowman-Birk isoinhibitor from pea (Pisum sativum L.), on HT29 colon cancer cells is mediated through protease inhibition. Br. J. Nutr. 2012, 108, 135-144.
20. Park, J.Y.; Kim, S.C.; Park, J.K.; Lee, S.J.; Choi, K.S.; Hahm, Y. Novel antibacterial activity of β(2)-microglobulin in human amniotic fluid. PLoS One 2012, 7, e47642.
21. Ozols, J. Amino acid analysis. In Guide to Protein Purification; Murray, P.D., Ed.; Methods in Enzymology Academic Press: London, UK, 1990; pp. 587-601.
22. Park, S.C.; Park, J.Y.; Kim, J.K.; Lee, I.; Hwang, H.; Cheong, J.W.; Nah, K.S.; Hahm, Y. Antifungal mechanism of a novel antifungal protein from pumpkin rinds against various fungal pathogens. J. Agric. Food Chem. 2009, 57, 9299-9304.
23. Park, S.; Yoo, J.Y.; Kim, S.C.; Park, Y.; do Choi, C.H.; Seo, K.S.; Hahm, Y. Effect of acidic pH on antibacterial action of peptide isolated from Korean pen shell (Atrina pectinata). J. Pept. Sci. 2011, 17, 353-357.
24. Park, S.C.; Park, J.Y.; Kim, C.; Jeong, S.; Yoo, K.S.; Hahm, Y. A plausible mode of action of pseudin-2, an antimicrobial peptide from Pseudis paradoxa. Biochim. Biophys. Acta 2011, 1808, 171-182.