First report of the protozoan parasite Perkinsus marinus in South America, infecting mangrove oysters Crassostrea rhizophorae from the Paraíba River (NE, Brazil)

Journal of Invertebrate Pathology

Volumn 113, Issue 1, 2013, Pages 96-103

  da Silva, Patricia Mirella ^a   Vianna, Rogério Tubino ^b   Guertler, Cristhiane ^b   Ferreira, Liana Pinho ^c   Santana, Lucas Nunes ^a   Fernández Boo, Sergio ^d   Ramilo, Andrea ^d   Cao, Asunción ^d   Villalba, Antonio ^d  

^a FEDERAL UNIVERSITY OF PARAÍBA   (Brazil)

^b FEDERAL UNIVERSITY OF SANTA CATARINA   (Brazil)

^c FEDERAL UNIVERSITY OF CEARÁ   (Brazil)

^d CENTRO DE INVESTIGACIÓNS MARIÑAS   (Spain)

Author keywords

Crassostrea rhizophorae; In vitro culture; Mangrove oyster; PCR; Perkinsus marinus; rDNA ITS

Indexed keywords

ALVEOLATA; ANIMAL; ARTICLE; BRAZIL; DNA SEQUENCE; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR CLONING; OYSTER; PARASITOLOGY; PHYLOGENY; PHYSIOLOGY; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

ALVEOLATA; ANIMALS; BRAZIL; CLONING, MOLECULAR; OSTREIDAE; PHYLOGENY; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SEQUENCE ANALYSIS, DNA;

EID: 84874943325     PISSN: 00222011     EISSN: 10960805     Source Type: Journal    
DOI: 10.1016/j.jip.2013.02.002     Document Type: Article

References (48)

1. Abollo, E., Casas, S.M., Ceschia, G., Villalba, A., Differential diagnosis of Perkinsus species by polymerase chain reaction–restriction fragment length polymorphism assay. Mol. Cell. Probes. 20 (2006), 323–329.
2. Boudry, P., Huertebise, S., Collet, B., Cornette, F., Gérard, A., Differentiation between populations of the Portuguese oyster Crassostrea angulata (Lamark) and the Pacific oyster Crassostrea gigas (Thunberg) revealed by mtDNA RFLP analysis. J. Exp. Mar. Biol. Ecol. 226 (1998), 279–291.
3. Brown, G.D., Hudson, K., Reece, K.S., Multiple polymorphic sites at the ITS and ATAN loci in cultured isolates of Perkinsus marinus. J. Eukariot. Microbiol. 51:3 (2004), 312–320.
4. Burreson, E.M., Reece, K.S., Dungan, C.F., Molecular, morphological, and experimental evidence support the synonymy of Perkinsus chesapeaki and Perkinsus andrewsi. J. Eukariot. Microbiol. 52:5 (2005), 258–270.
5. Bush, A.O., Lafferty, K.D., Lotz, J.M., Shostak, A.W., Parasitology meets ecology on its own terms: Margolis et al. revisited. J. Parasitol. 83 (1997), 575–583.
6. Bushek, D., Scarpa, J., Laramore, S.E., Susceptibility of the Caribbean oyster Crassostrea rhizophorae to Perkinsus marinus (abstract). J. Shellfish Res. 21 (2002), 371–372.
7. Cáceres-Martínez, J., Vásquez-Yeomans, R., Padilla-Lardizábal, G., del Río Portilla, M.A., Perkinsus marinus in pleasure oyster Crassostrea corteziensis from Nayarit, Pacific coast of Mexico. J. Invertebr. Pathol. 99 (2008), 66–73.
8. Calvo, G.W., Luckenbach, M.W., Allen, S.K., Burreson, E.M., A comparative field study of Crassostrea ariakensis (Fujita 1913) and Crassostrea virginica (Gmelin 1791) in relation to salinity in Virginia. J. Shellfish Res. 20 (2001), 221–229.
9. Casas, S.M., La Peyre, J.F., Reece, K.S., Azevedo, C., Villalba, A., Continuous in vitro culture of the carpet shell clam Tapes decussatus protozoan parasite Perkinsus atlanticus. Dis. Aquat. Org. 52 (2002), 217–231.
10. Casas, S.M., Villalba, A., Reece, K.S., Study of perkinsosis in the carpet shell clam Tapes decussatus in Galicia (NW Spain). I. Identification of the aetiological agent and in vitro modulation of zoosporulation by temperature and salinity. Dis. Aquat. Org. 50 (2002), 51–65.
11. Casas, S.M., Grau, A., Reece, K.S., Apakupakul, K., Azevedo, C., Villalba, A., Perkinsus mediterraneus n. sp., a protistan parasite of the European flat oyster Ostrea edulis from the Balearic Island. Mediterranean Sea. Dis. Aquat. Org. 58 (2004), 231–244.
12. Choi, K.S., Park, K.I., 2010. Review on the protozoan parasite Perkinsus olseni (Lester and Davis 1981) infection in Asian waters. In: Ishimatsu, A., Lie, H.J. (Eds.), Coastal Environmental and Ecosystem Issues of the East China Sea. Terrapub and Nagasaki University, pp. 269–281.
13. Coss, C.A., Robledo, J.A.F., Ruiz, G.M., Vasta, G.R., Description of Perkinsus andrewsi n. sp. isolated from the Baltic clam (Macoma balthica) by characterization of the ribosomal RNA locus, and development of a species-specific PCR-based diagnostic assay. J. Eukaryot. Microbiol. 48 (2001), 52–61.
14. Cremonte, F., Balseiro, P., Figueras, A., Occurrence of Perkinsus olseni (Protozoa: Apicomplexa) and other parasites in the venerid commercial clam Pitar rostrata from Uruguay southwestern Atlantic coast. Dis. Aquat. Org. 64 (2005), 85–90.
15. da Silva, P.M., Magalhães, A.R.M., Barracco, M.A., Pathologies in commercial bivalve species from Santa Catarina State southern Brazil. J. Mar. Biol. Assoc. UK. 92 (2012), 571–579.
16. de Melo, A.G.C., Varela, E.S., Beasley, C.R., Schneider, H., Sampaio, I., Gaffney, P.M., Reece, K.S., Tagliaro, C.H., Molecular identification, phylogeny and geographic distribution of Brazilian mangrove oysters (Crassostrea). Genet. Mol. Biol. 33 (2010), 564–572.
17. Dungan, F.C., Reece, K.S., In vitro propagation of two Perkinsus spp. parasites from Japanese Manila clams Venerupis philippinarum and description of Perkinsus honshuensis n. sp. J. Eukariot. Microbiol. 53:5 (2006), 316–326.
18. Enriquez-Espinoza, T.L., Grijalva-Chon, J.M., Castro-Longoria, R., Ramos-Paredes, J., Perkinsus marinus in Crassostrea gigas in the Gulf of California. Dis. Aquat. Org. 89 (2010), 269–273.
19. Gauthier, J., Vasta, G., In vitro culture of the eastern oyster parasite Perkinsus marinus: optimization of the methodology. J. Invertebr. Pathol. 66 (1995), 156–158.
20. Hall, T.A., BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41 (1999), 95–98.
21. Huelsenbeck, J.P., Ronquist, F., MRBAYES: Bayesian inference of phylogeny. Bioinformatics 17 (2001), 754–755.
22. Ignacio, B.L., Absher, T.M., Lazoski, C., Solé-Cava, A.M., Genetic evidence of the presence of two species of Crassostrea (Bivalvia: Ostreidae) on the coast of Brazil. Mar. Biol. 136 (2000), 987–991.
23. Kessing, B., Croom, H., Martin, A., McIntosh, C., Owen, M., Palumbi, S., The simple fool's guide to PCR. 1989, Department of Zoology, University of Hawaii, Havaí.
24. La Peyre, J.F., Faisal, M., Burreson, E.M., In vitro propagation of the protozoan Perkinsus marinus, a pathogen of the eastern oyster, Crassostrea virginica. J. Eukaryot. Microbiol. 40 (1993), 304–310.
25. Lapègue, S., Boutet, I., Leitão, A., Heurtebise, S., Garcia, P., Thiriot-Quiévreux, C., Boudry, P., Trans-Atlantic distribution of a mangrove oyster species revealed by 16S mtDNA and karyological analyses. Biol. Bull. 202 (2002), 232–242.
26. Lazoski, C., 2004. Sistemática molecular e genética populacional de ostras brasileiras (Crassostrea spp.). Ph.D. Thesis, Universidade Federal do Rio de Janeiro. Rio de Janeiro, Brazil.
27. Littlewood, D.T.J., First report of the Protozoan Perkinsus cf. marinus in the mangrove oyster Crassostrea rhizophorae (Guilding). Caribbean J. Sci. 36 (2000), 153–154.
28. Mackin, J.G., Histopathology of infection of Crassostrea virginica (Gmelin) by Dermocystidium marinum Mackin, Owen and Collier. B. Mar. Sci. Gulf Caribbean. 1 (1951), 72–87.
29. Ministério da Pesca e Aquicultura, 2012. Boletim Estatístico da Pesca e aquicultura: Brasil 2010. Brasília, DF.
30. Moss, J.A., Burreson, E.M., Reece, K.S., Advanced Perkinsus marinus infections in Crassostrea ariakensis maintained under laboratory conditions. J. Shellfish Res. 25 (2006), 65–72.
31. Moss, J.A., Xiao, J., Dungan, C.F., Reece, K.S., Description of Perkinsus beihaiensis n. sp., a new Perkinsus sp. parasite in oysters of southern China. J. Eukaryot. Microbiol. 55 (2008), 117–130.
32. OIE, 2012. Infection with Perkinsus marinus. In: Manual of Diagnostic Tests for Aquatic Animals. May 24, 2012. < http://www.oie.int/fileadmin/Home/eng/Health_standards/aahm/2010/2.4.05_P_MARINUS.pdf>.
33. Park, K.I., Ngo, T.T.T., Choi, S.D., Cho, M., Choi, K.S., Occurrence of Perkinsus olseni in the Venus clam Protothaca jedoensis in Korean waters. J. Invertebr. Pathol. 93 (2006), 81–87.
34. Pecher, W.T., Alawi, M.R., Schott, E.J.J., Robledo, A.F., Roth, L., et al. Assessment of the northern distribution range of selected Perkinsus species in eastern oysters (Crassostrea virginica) and hard clams (Mercenaria mercenaria) with the use of PCR-based detection assays. J. Parasitol. 94 (2008), 410–422.
35. Pie, M.R., Ribeiro, R.O., Boeger, W.A., Ostrensky, A., Falleiros, R.M., Angelo, L., A simple PCR–RFLP method for the discrimination of native and introduced oyster species (Crassostrea brasiliana. C. rhizophorae and C. gigas; Bivalvia. Ostreidae) cultured in Southern Brazil. Aquac. Res. 37 (2006), 1598–1600.
36. Posada, D., Crandall, K.A., MODELTEST: testing the model of DNA substitution. Bioinformatics 14 (1998), 817–818.
37. Ray, S.M., Biological studies of Dermocystidium marinum. Rice Inst. Pamphlet 41 (1954), 1–114 (special issue).
38. Ray, S.M., A review of the culture method of detecting Dermocystidium marinum with suggested modifications and precautions. Proc. Natl. Shellfish Assoc. 54 (1966), 55–69.
39. Reece, K.S., Dungan, C.F., Burreson, E.M., Molecular epizootiology of Perkinsus marinus and P. chesapeaki infections among wild oysters and clams in Chesapeake Bay, USA. Dis. Aquat. Org. 82 (2008), 237–248.
40. Rios, E.C., Compendium of Brazilian Sea Shells. 2009, Ed Evangraf, Rio Grande.
41. Sabry, R.C., Rosa, R.D., Magalhães, A.R.M., Barracco, M.A., Gesteira, T.C.V., da Silva, P.M., First report of Perkinsus sp. infecting mangrove oysters Crassostrea rhizophorae from the Brazilian coast. Dis. Aquat. Org. 88 (2009), 13–23.
42. Sabry, R.C., da Silva, P.M., Gesteira, T.C.V., Pontinha, V.A., Magalhães, A.R.M., Pathological study of oysters Crassostrea gigas from culture and C. rhizophorae from natural stock of Santa Catarina Island, SC, Brazil. Aquaculture 60 (2011), 43–50.
43. Sabry, R.C., Gesteira, T.C.V., Magalhães, A.R.M., Barracco, M.A., Guertler, C., Ferreira, L.P., Vianna, R.T., da Silva, P.M., Parasitological survey of mangrove oyster, Crassostrea rhizophorae, in the Pacoti River Estuary, Ceará State, Brazil. J. Invertebr. Pathol. 112 (2013), 24–32.
44. Sanil, N.K., Suja, G., Lijo, J., Vijayan, K.K., First report of Perkinsus beihaiensis in Crassostrea madrasensis from the Indian subcontinent. Dis. Aquat. Org. 98 (2012), 209–220.
45. Swofford, D.L., PAUP 4.0: Phylogenetic Analysis Using Parsimony (and Other Methods). 2002, Sinauer Associates Inc., Sunderland, MA, USA.
46. Thompson, J.D., Higgins, D.G., Gibson, T.J., CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting. Position specific gap penalties and weight matrix choice. Nucleic Acids Res. 22 (1994), 4673–4680.
47. Villalba, A., Reece, K.S., Ordás, M.C., Casas, S.M., Figueras, A., Perkinsosis in molluscs: a review. Aquat. Living Resour. 17 (2004), 411–432.
48. Villalba, A., Gestal, C., Casas, S.M., Figueras, A., 2011. Perkinsosis en moluscos. In. Figueras, A., Novoa, B. (Eds.), Enfermedades de Moluscos Bivalvos de Interés en Acuicultura. Fundación Observatorio Español de Acuicultura, Madrid. pp. 181–242.