Molecular epizootiology of Perkinsus marinus and P. chesapeaki infections among wild oysters and clams in Chesapeake Bay, USA

Diseases of Aquatic Organisms

Volumn 82, Issue 3, 2008, Pages 237-248

  Reece, Kimberly S ^a   Dungan, Christopher F ^b   Burreson, Eugene M ^a  

^a VIRGINIA INSTITUTE OF MARINE SCIENCE   (United States)

^b MARYLAND DEPARTMENT OF NATURAL RESOURCES   (United States)

Author keywords

Diagnostic assays; Histology; In situ hybridization; In vitro isolates; Internal transcribed spacer; ITS; Large subunit ribosomal RNA; LSU rRNA; Parasite; PCR

Indexed keywords

ASSAY; BIVALVE; DINOFLAGELLATE; DISEASE PREVALENCE; DNA; EXPERIMENTAL STUDY; HISTOLOGY; HOST-PARASITE INTERACTION; INFECTIOUS DISEASE; POLYMERASE CHAIN REACTION; RNA; SYMPATRY;

ANIMAL; ARTICLE; BIVALVE; HOST PARASITE INTERACTION; ISOLATION AND PURIFICATION; PARASITOLOGY; PHYSIOLOGY; PROTOZOON;

ANIMALS; BIVALVIA; HOST-PARASITE INTERACTIONS; PROTOZOA;

CHESAPEAKE BAY; NORTH AMERICA; UNITED STATES;

BIVALVIA; CRASSOSTREA VIRGINICA; CYRTOPLEURA COSTATA; MACOMA BALTHICA; MOLLUSCA; MULINIA LATERALIS; MYA; MYA ARENARIA; OSTREIDAE; PERKINSUS; PERKINSUS CHESAPEAKI; PERKINSUS MARINUS; PERKINSUS SP.; RANGIA CUNEATA; TAGELUS PLEBEIUS;

EID: 58949085190     PISSN: 01775103     EISSN: 16161580     Source Type: Journal    
DOI: 10.3354/dao01997     Document Type: Article

References (36)

1. Andrews JD (1954) Notes on fungus parasites of bivalve mollusks in Chesapeake Bay. Proc Natl Shellfish Assoc 45:157-163
2. Audemard C, Reece KS, Burreson EM (2004) Real-time PCR for detection and quantification of the protistan parasite Perkinsus marinus in environmental waters. Appl Environ Microbiol 70:6611-6618
3. Burreson EM (2008) Misuse of PCR assay for diagnosis of mollusc protistan infections. Dis Aquat Org 80:81-83
4. Burreson EM, Reece KS, Dungan CF (2005) Molecular, morphological, and experimental evidence support the synonymy of Perkinsus chesapeaki and Perkinsus andrewsi. J Eukaryot Microbiol 52:258-270
5. Bush AO, Lafferty KD, Lotz JM, Shostak AW (1997) Parasitology meets ecology on its own terms: Margolis et al. revisited. J Parasitol 83:575-583
6. Bushek D, Landau B, Scarpa E (2008) Perkinsus chesapeaki in stout razor clams Tagelus plebeius from Delaware Bay. Dis Aquat Org 78:243-247
7. Carnegie RB, Burreson EM, Hine PM, Stokes NA, Audemard C, Bishop MJ, Peterson CH (2006) Bonamia perspora n. sp. (Haplosporidia), a parasite of the oyster Ostreola equestris, is the first Bonamia species known to produce spores. J Eukaryot Microbiol 53:232-245
8. Casas SM, Villalba A, Reece KS (2002) Study of perkinsosis in the carpet shell clam Tapes decussatus in Galicia (NW Spain). I. Identification of the aetiological agent and in vitro modulation of zoosporulation by temperature and salinity. Dis Aquat Org 50:51-63
9. Coss CA, Robledo JAF, Ruiz GM, Vasta GR (2001) Description of Perkinsus andrewsi n. sp. isolated from the Baltic clam (Macoma balthica) by characterization of the ribosomal RNA locus, and development of a species-specific PCR-based diagnostic assay. J Eukaryot Microbiol 48:52-61
10. Dungan CF, Hamilton RM, Hudson KL, McCollough CB, Reece KS (2002) Two epizootic diseases in Chesapeake Bay commercial clams Mya arenaria and Tagelus plebeius. Dis Aquat Org 50:67-78
11. Dungan CF, Hamilton RM, Reece KS, Burreson EM (2007) Experimental cross-infections by Perkinsus marinus and P. chesapeaki in three species of sympatric Chesapeake Bay oysters and clams. Dis Aquat Org 76:67-75
12. Elston RA, Dungan CF, Meyers TR, Reece KS (2004) Perkinsus sp. infection risk for Manila clams, Venerupis philippinarum (A. Adams and Reeve, 1850) on the Pacific coast of North and Central America. J Shellfish Res 23:101-105
13. Ford SE (2001) Pests, parasites, diseases, and defense mechanisms of the hard clam, Mercenaria mercenaria. In: Kraeuter JN, Castagna M (eds) Biology of the hard clam. Elsevier, Amsterdam, p 591-628
14. Gauthier JD, Miller CR, Wilbur AE (2006) Taqman MGB real-time PCR approach to quantification of Perkinsus marinus and Perkinsus spp. in oysters. J Shellfish Res 25:619-624
15. Kotob SI, McLaughlin SM, van Berkum P, Faisal M (1999) Characterization of two Perkinsus spp. from the softshell clam, Mya arenaria, using the small subunit ribosomal RNA gene. J Eukaryot Microbiol 46:439-444
16. La Peyre JF, Faisal M (1995) Improved method for the initiation of continuous cultures of the oyster pathogen Perkinsus marinus (Apicomplexa). Trans Am Fish Soc 124:144-146
17. La Peyre M, Casas S, La Peyre J (2006) Salinity effects on viability, metabolic activity, and cellular proliferation of three Perkinsus spp. Dis Aquat Org 71:59-74
18. Lenaers G, Maroteaux L, Michot B, Herzog M (1989) Dinoflagellates in evolution. A molecular phylogenetic analysis of large subunit ribosomal RNA. J Mol Evol 29:40-51
19. Mackin JG, Owen HM, Collier A (1950) Preliminary note on the occurrence of new protistan parasite, Dermocystidium marinum n. sp. in Crassostrea virginica (Gmelin). Science 111:328-329
20. Marsh AG, Gauthier JD, Vasta GR (1995) Semiquantitative PCR assay for assessing Perkinsus marinus infections in the eastern oyster, Crassostrea virginica. J Parasitol 81: 577-583
21. McCoy A, Baker SM, Wright AC (2007) Investigation of Perkinsus spp. in aquacultured hard clams (Mercenaria mercenaria) from the Florida Gulf coast. J Shellfish Res 26:1029-1033
22. McLaughlin SM, Faisal M (2000) Prevalence of Perkinsus spp. in Chesapeake Bay soft-shell clams, Mya arenaria Linneaeus, 1758 during 1990-1998. J Shellfish Res 19: 349-352
23. McLaughlin SM, Tall BD, Shaheen A, El Sayed EE, Faisal M (2000) Zoosporulation of a new Perkinsus species isolated from the gills of the softshell clam Mya arenaria. Parasite 7:115-122
24. Moss JA, Burreson EM, Reece KS (2006) Advanced Perkinsus marinus infections in Crassostrea ariakensis maintained under laboratory conditions. J Shellfish Res 25:65-72
25. Pecher WT, Alavi MR, Schott EJ, Feranadez-Robledo JA, Roth L, Berg ST, Vasta GR (2008) Assessment of the northern distribution range of selected Perkinsus species in eastern oysters (Crassostrea virginica) and hard clams (Mercenaria mercenaria) with the use of PCR-based detection assays. J Parasitol 94:410-422
26. Perkins FO (1996) The structure of Perkinsus marinus (Mackin, Owen and Collier, 1950) Levine, 1978 with comments on taxonomy and phytogeny of Perkinsus spp. J Shellfish Res 15:67-87
27. Ragone Calvo LM, Dungan CF, Roberson BS, Burreson EM (2003) Systematic evaluation of factors controlling Perkinsus marinus transmission dynamics in lower Chesapeake Bay. Dis Aquat Org 56:75-86
28. Ray SM (1952) A culture technique for the diagnosis of infections by Dermocystidium marinum Mackin, Owen, and Collier in oysters. Science 116:360-361
29. Ray SM (1954) Biological studies of Dermocystidium marinum, a fungus parasite of oysters. The Rice Institute, Houston, TX
30. Ray SM, Chandler AC (1955) Dermocystidium marinum, a parasite of oysters. Exp Parasitol 4:172-200
31. Reece KS, Stokes NA (2003) Molecular analysis of a haplosporidian parasite from cultured New Zealand abalone Haliotis iris. Dis Aquat Org 53:61-66
32. Robledo JAF, Gauthier JD, Coss CA, Wright AC, Vasta GR (1998) Species-specificity and sensitivity of a PCR-based assay for Perkinsus marinus in the eastern oyster, Crassostrea virginica: a comparison with the fluid thioglycollate assay. J Parasitol 84:1237-1244
33. Shaw BL, Battle HI (1957) The gross and microscopic anatomy of the digestive tract of the oyster Crassostrea virginica (Gmelin). Can J Zool 35:325-347
34. Stokes NA, Burreson EM (1995) A sensitive and specific DNA probe for the oyster pathogen Haplosporidium nelsoni. J Eukaryot Microbiol 42:350-357
35. Valiulis GA, Mackin JG (1969) Formation of sporangia and zoospores by Labyrinthomyxa sp. parasitic in the clam Macoma balthica. J Invertebr Pathol 14:268-270
36. Yarnall HA, Reece KS, Stokes NA, Burreson EM (2000) A quantitative competitive polymerase chain reaction assay for the oyster pathogen Perkinsus marinus. J Parasitol 86:827-837