Ex vivo electroporation of retinal cells: A novel, high efficiency method for functional studies in primary retinal cultures

Experimental Eye Research

Volumn 109, Issue , 2013, Pages 40-50

  Vergara, M Natalia ^a   Gutierrez, Christian ^a   O'Brien, David R ^a   Canto Soler, M Valeria ^a  

^a JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

Chick; Culture; Electroporation; Gain of function; In vitro; Loss of function; Photoreceptor; Retina

Indexed keywords

ETHIDIUM; HOMODIMER; TRANSCRIPTION FACTOR PAX6; VISININ;

ANIMAL CELL; CELL DIFFERENTIATION; CELL SURVIVAL; CELL VIABILITY; CHICK; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DEVELOPMENTAL STAGE; ELECTRODE; ELECTROPORATION; EX VIVO STUDY; GENETIC TRANSFECTION; HIGH THROUGHPUT SCREENING; HISTOPATHOLOGY; IMMUNOCYTOCHEMISTRY; INCUBATION TIME; LOSS OF FUNCTION MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHOTORECEPTOR; PHOTORECEPTOR CELL; PIGMENT EPITHELIUM; PRIORITY JOURNAL; PROTEIN BLOOD LEVEL; PROTEIN EXPRESSION; PROTEIN FUNCTION; REAL TIME POLYMERASE CHAIN REACTION; RETINA CELL; RNA INTERFERENCE; SHORT SURVEY; UPREGULATION; WESTERN BLOTTING;

ANIMALS; CELL COUNT; CHICK EMBRYO; CHICKENS; ELECTROPORATION; EYE PROTEINS; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GREEN FLUORESCENT PROTEINS; HOMEODOMAIN PROTEINS; PAIRED BOX TRANSCRIPTION FACTORS; PLASMIDS; PRIMARY CELL CULTURE; REPRESSOR PROTEINS; RETINA; TRANSFECTION;

EID: 84874754697     PISSN: 00144835     EISSN: 10960007     Source Type: Journal    
DOI: 10.1016/j.exer.2013.01.010     Document Type: Short Survey

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