The Xenopus oocyte: A single-cell model for studying Ca2+ signaling

Cold Spring Harbor Protocols

Volumn 8, Issue 3, 2013, Pages 185-191

  Lin Moshier, Yaping ^a   Marchant, Jonathan S ^a  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; CALCIUM CHANNEL; MESSENGER RNA;

ANIMAL; ARTICLE; BIOLOGICAL MODEL; CALCIUM SIGNALING; CYTOLOGY; HETEROLOGOUS EXPRESSION; METABOLISM; METHODOLOGY; OOCYTE; OOCYTE DEVELOPMENT; PHYSIOLOGY; RNA TRANSLATION; SIGNAL TRANSDUCTION; SINGLE CELL ANALYSIS; XENOPUS; XENOPUS LAEVIS;

ANIMALS; CALCIUM; CYTOLOGICAL TECHNIQUES; METABOLISM; METHODS; OOCYTES; PHYSIOLOGY; SIGNAL TRANSDUCTION; XENOPUS;

EID: 84874704380     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.top066308     Document Type: Article

References (91)

1. 2X receptor from Schistosoma mansoni. J Biol Chem 279: 41650-41657.
2. Anderson DM, Smith LD. 1978. Patterns of synthesis and accumulation of heterogeneous RNA in lampbrush stage oocytes of Xenopus laevis (Daudin). Dev Biol 67: 274-285.
3. Anderson DM, Richter JD, Chamberlin ME, Price DH, Britten RJ, Smith LD, Davidson EH. 1982. Sequence organization of the poly(A) RNA synthesized and accumulated in lampbrush chromosome stage Xenopus laevis oocytes. J Mol Biol 155: 281-309.
4. Antoine AF, Montpellier C, Cailliau K, Browaeys-Poly E, Vilain JP, Dubuisson J. 2007. The alphavirus 6K protein activates endogenous ionic conductances when expressed in Xenopus oocytes. J Membr Biol 215: 37-48.
5. Au S, Cohen S, Pante N. 2010. Microinjection of Xenopus laevis oocytes as a system for studying nuclear transport of viruses. Methods 51: 114-120.
6. Barnard EA, Miledi R, Sumikawa K. 1982. Translation of exogenous messengerRNAcoding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes. Proc R Soc Lond B Biol Sci 215: 241-246.
7. Beene DL, Dougherty DA, Lester HA. 2003. Unnatural amino acid mutagenesis in mapping ion channel function. Curr Opin Neurobiol 13: 264-270.
8. Bianchi L, Gerstbrein B, Frokjaer-Jensen C, Royal DC, Mukherjee G, Royal MA, Xue J, Schafer WR, Driscoll M. 2004. The neurotoxic MEC-4(d) DEG/ENaC sodium channel conducts calcium: implications for necrosis initiation. Nat Neurosci 7: 1337-1344.
9. Bocquet N, Prado de Carvalho L, Cartaud J, Neyton J, Le Poupon C, Taly A, Grutter T, Changeux JP, Corringer PJ. 2007. A prokaryotic protongated ion channel from the nicotinic acetylcholine receptor family. Nature 445: 116-119.
10. Boulter E, Grall D, Cagnol S, Van Obberghen-Schilling E. 2006. Regulation of cell-matrix adhesion dynamics and Rac-1 by integrin linked kinase. FASEB J 20: 1489-1491.
11. Boulware MJ, Marchant JS. 2005. IP3 receptor activity is differentially regulated in endoplasmic reticulum subdomains during oocyte maturation. Curr Biol 15: 765-770.
12. 2+ signaling competency. J Physiol (Lond) 586: 2873-2888.
13. Bowes JB, Snyder KA, Segerdell E, Jarabek CJ, Azam K, Zorn AM, Vize PD. 2010. Xenbase: gene expression and improved integration. Nucleic Acids Res 38: D607-612.
14. Brailoiu E, Hooper R, Cai X, Brailoiu GC, Keebler MV, Dun NJ, Marchant JS, Patel S. 2009. An ancestral deuterostome family of two-pore channels mediates nicotinic acid adenine dinucleotide phosphate-dependent calcium release from acidic organelles. J Biol Chem 285: 2897-2901.
15. Brown DD. 2004. A tribute to the Xenopus laevis oocyte and egg. J Biol Chem 279: 45291-45299.
16. Brown DD, Dawid IB. 1968. Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA. Science 160: 272-280.
17. Callen JC, Tourte M, Dennebouy N, Mounolou JC. 1980. Mitochondrial development in oocytes of Xenopus laevis. Biol Cell 38: 13-18.
18. Camacho P, Lechleiter JD. 1993. Increased frequency of calcium waves in Xenopus laevis oocytes that express a calcium-ATPase. Science 260: 226-229.
19. Chase JW, Dawid IB. 1972. Biogenesis of mitochondria during Xenopus laevis development. Dev Biol 27: 504-518.
20. Choi SB, Kim JU, Joo H, Min CK. 2010. Identification and characterization of a novel bacterial ATP-sensitive K+ channel. J Microbiol 48: 325-330.
21. Cordes VC, Reidenbach S, FrankeWW.1995. High content of a nuclear pore complex protein in cytoplasmic annulate lamellae of Xenopus oocytes. Eur J Cell Biol 68: 240-255.
22. Demuro A, Parker I. 2006. Imaging single-channel calcium microdomains. Cell Calcium 40: 413-422.
23. Dumont JN. 1972. Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals. J Morphol 136: 153-180.
24. Eckstein-Ludwig U, Webb RJ, Van Goethem ID, East JM, Lee AG, Kimura M, O'Neill PM, Bray PG, Ward SA, Krishna S. 2003. Artemisinins target the SERCA of Plasmodium falciparum. Nature 424: 957-961.
25. Fletcher JS, Lockyer NP, Vaidyanathan S, Vickerman JC. 2007. TOF-SIMS 3D biomolecular imaging of Xenopus laevis oocytes using buckminsterfullerene (C60) primary ions. Anal Chem 79: 2199-2206.
26. Fujioka A, Terai K, Itoh RE, Aoki K, Nakamura T, Kuroda S, Nishida E, Matsuda M. 2006. Dynamics of the Ras/ERK MAPK cascade as monitored by fluorescent probes. J Biol Chem 281: 8917-8926.
27. Geib S, Sandoz G, Carlier E, Cornet V, Cheynet-Sauvion V, De Waard M. 2001. A novel Xenopus oocyte expression system based on cytoplasmic coinjection of T7-driven plasmids and purified T7-RNA polymerase. Receptors Channels 7: 331-343.
28. Girard S, Clapham DE. 1993. Acceleration of intracellular calcium waves in Xenopus oocytes by calcium influx. Science 260: 229-232.
29. Goldin AL. 2006. Expression of ion channels in Xenopus oocytes. In Expression and analysis of recombinant ion channels (ed. Clare JJ, Trezise DJ), pp. 1-25. Wiley-VCH.
30. Gurdon JB, Lane CD, Woodland HR, Marbaix G. 1971. Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells. Nature 233: 177-182.
31. Hallberg RL, Smith DC. 1975. Ribosomal protein synthesis in Xenopus laevis oocytes. Dev Biol 42: 40-52.
32. Halley-Stott RP, Pasque V, Astrand C, Miyamoto K, Simeoni I, Jullien J, Gurdon JB. 2010. Mammalian nuclear transplantation to germinal vesicle stage Xenopus oocytes-A method for quantitative transcriptional reprogramming. Methods 51: 56-65.
33. Harland RM, Grainger RM. 2011. Xenopus research: Metamorphosed by genetics and genomics. Trends Genet 27: 507-515.
34. Izumi-Nakaseko H, Yamaguchi S, Ohtsuka Y, Ebihara T, Adachi-Akahane S, Okamura Y. 2003. DHP-insensitive L-type-like Ca channel of ascidian acquires sensitivity to DHP with single amino acid change in domain III P-region. FEBS Lett 549: 67-71.
35. Jeziorski MC, Greenberg RM, Clark KS, Anderson PA. 1998. Cloning and functional expression of a voltage-gated calcium channel α1 subunit from jellyfish. J Biol Chem 273: 22792-22799.
36. Jouaville LS, Ichas F, Holmuhamedov EL, Camacho P, Lechleiter JD. 1995. Synchronization of calcium waves by mitochondrial substrates in Xenopus laevis oocytes. Nature 377: 438-441.
37. Kimura T, Kubo T. 2002. Functional identification of a cloned squid presynaptic voltage-dependent calcium channel. Neuroreport 13: 2389-2393.
38. Kume S, Muto A, Inoue T, Suga K, Okano H, Mikoshiba K. 1997. Role of the inositol 1,4,5-trisphosphate receptor in ventral signaling in Xenopus embryos. Science 278: 1940-1943.
39. Lan WZ, Wang W, Wang SM, Li LG, Buchanan BB, Lin HX, Gao JP, Luan S. 2010. A rice high-affinity potassium transporter (HKT) conceals a calcium-permeable cation channel. Proc Natl Acad Sci 107: 7089-7094.
40. Le Caherec F, Bron P, Verbavatz JM, Garret A, Morel G, Cavalier A, Bonnec G, Thomas D, Gouranton J, Hubert JF. 1996. Incorporation of proteins into (Xenopus) oocytes by proteoliposome microinjection: Functional characterization of a novel aquaporin. J Cell Sci 109: 1285-1295.
41. Lechleiter JD, Girard S, Clapham D, Peralta E. 1991a. Subcellular patterns of calcium release determined by G protein-specific residues of muscarinic receptors. Nature 350: 505-508.
42. Lechleiter JD, Girard S, Peralta E, Clapham D. 1991b. Spiral calcium wave propagation and annihilation in Xenopus laevis oocytes. Science 252: 123-126.
43. Lee SC, Cho JH, Mietchen D, Kim YS, Hong KS, Lee C, Kang D, Park KD, Choi BS, Cheong C. 2006. Subcellular in vivo 1H MR spectroscopy of Xenopus laevis oocytes. Biophys J 90: 1797-1803.
44. Legros F, Malka F, Frachon P, Lombes A, Rojo M. 2004. Organization and dynamics of human mitochondrial DNA. J Cell Sci 117: 2653-2662.
45. Lenart P, Ellenberg J. 2003. Nuclear envelope breakdown in oocytes: from germinal vesicle breakdown to mitosis. Curr Opin Cell Biol 15: 88-95.
46. Lesage F, Guillemare E, Fink M, Duprat F, Lazdunski M, Romey G, Barhanin J. 1996. A pH-sensitive yeast outward rectifier K+ channel with two pore domains and novel gating properties. J Biol Chem 271: 4183-4187.
47. 2+ signals in Xenopus oocytes. Cold Spring Harb Protoc doi: 10.1101/pdb.prot072785.
48. Lin-Moshier Y, Marchant JS. 2013b. A rapid western blotting protocol for the Xenopus oocyte. Cold Spring Harb Protoc doi: 10.1101/pdb. prot072793.
49. Lubbert H, Hoffman BJ, Snutch TP, van Dyke T, Levine AJ, Hartig PR, Lester HA, Davidson N. 1987. cDNA cloning of a serotonin 5-HT1C receptor by electrophysiological assays of mRNA-injected Xenopus oocytes. Proc Natl Acad Sci USA 84: 4332-4336.
50. 2X receptors. J Biol Chem 284: 35227-35239.
51. Mak D-OD, Foskett JK. 1994. Single channel inositol 1,4,5-trisphosphate receptor currents revealed by patch clamp of isolated Xenopus oocyte nuclei. J Biol Chem 269: 29375-29378.
52. Maksaev G, Haswell ES. 2011. Expression and characterization of the bacterial mechanosensitive channel MscS in Xenopus laevis oocytes. J Gen Physiol 138: 641-649.
53. 2+ oscillations. EMBO J 20: 65-76.
54. 2+ signalling. Br J Pharmacol 132: 1396-1410.
55. Marinos E. 1985. The number of mitochondria in Xenopus laevis ovulated oocytes. Cellular Differentiation 16: 139-143.
56. Markovich D. 2008. Expression cloning and radiotracer uptakes in Xenopus laevis oocytes. Nature Protocols 3: 1975-1980.
57. Miledi R, Parker I. 1984. Chloride current induced by injection of calcium into Xenopus oocytes. J Physiol (Lond) 357: 173-183.
58. Miledi R, Parker I, Sumikawa K. 1983. Recording of single gamma-aminobutyrate-and acetylcholine-activated receptor channels translated by exogenous mRNA in Xenopus oocytes. Proc R Soc Lond B Biol Sci 218: 481-484.
59. Moar VA, Gurdon JB, Lane CD, Marbaix G. 1971. Translational capacity of living frog eggs and oocytes, as judged by messenger RNA injection. J Mol Biol 61: 93-103.
60. Mosimann M, Goshima S, Wenzler T, Luscher A, Uozumi N, Maser P. 2010. A Trk/HKT-type K+ transporter from Trypanosoma brucei. Eukaryotic Cell 9: 539-546.
61. Nagel G, Szellas T, Huhn W, Kateriya S, Adeishvili N, Berthold P, Ollig D, Hegemann P, Bamberg E. 2003. Channelrhodopsin-2, a directly lightgated cation-selective membrane channel. Proc Natl Acad Sci 100: 13940-13945.
62. Nielsen PJ, Thomas G, Maller JL. 1982. Increased phosphorylation of ribosomal protein S6 during meiotic maturation of Xenopus oocytes. Proc Natl Acad Sci U S A 79: 2937-2941.
63. Ottolia M, Philipson KD, John S. 2007. Xenopus oocyte plasma membrane sheets for FRET analysis. Am J Physiol Cell Physiol 292: C1519-1522.
64. Palma E, Trettel F, Fucile S, Renzi M, Miledi R, Eusebi F. 2003. Microtransplantation of membranes from cultured cells to Xenopus oocytes: A method to study neurotransmitter receptors embedded in native lipids. Proc Natl Acad Sci 100: 2896-2900.
65. Papke RL, Stokes C. 2010. Working with OpusXpress: Methods for high volume oocyte experiments. Methods 51: 121-133.
66. Parker I, Yao Y. 1991. Regenerative release of calcium from functionally discrete subcellular stores by inositol trisphosphate. Proc R Soc Lond B Biol Sci 246: 269-274.
67. 2+ liberation in Xenopus oocytes: Hot spots, puffs and blips. Cell Calcium 20: 105-121.
68. Parys JB, Sernett SW, DeLisle S, Snyder PM, Welsh MJ, Campbell KP. 1992. Isolation, characterization and localization of the inositol 1,4,5-trisphosphate receptor protein in Xenopus laevis oocytes. J Biol Chem 267: 18776-18782.
69. Pearl EJ, Grainger RM, Guille M, Horb ME. 2012. Development of Xenopus resource centers: The national Xenopus resource and the European Xenopus resource center. Genesis 50: 155-163.
70. Peters R. 2006. Use of Xenopus laevis oocyte nuclei and nuclear envelopes in nucleocytoplasmic transport studies. Methods Mol Biol 322: 259-272.
71. Roy SJ, Gilliham M, Berger B, Essah PA, Cheffings C, Miller AJ, Davenport RJ, Liu LH, Skynner MJ, Davies JM, et al. 2008. Investigating glutamate receptor-like gene co-expression in Arabidopsis thaliana. Plant, Cell Environ 31: 861-871.
72. Saneyoshi T, Kume S, Amasaki Y, Mikoshiba K. 2002. The Wnt/calcium pathway activates NF/AT and promotes ventral cell fate in Xenopus embryos. Nature 417: 295-299.
73. Schroeder BC, Cheng T, Jan YN, Jan LY. 2008. Expression cloning of TMEM16A as a calcium-activated chloride channel subunit. Cell 134: 1019-1029.
74. 2+ concentration in rat heart mitochondria. J Physiol 518(Pt 2): 577-584.
75. Sigel E. 1990. Use of Xenopus oocytes for the functional expression of plasma membrane proteins. J Membr Biol 117: 201-221.
76. Sims CE, Allbritton NL. 2007. Analysis of single mammalian cells on-chip. Lab Chip 7: 423-440.
77. Sindelka R, Sidova M, Svec D, Kubista M. 2010. Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography. Methods 51: 87-91.
78. Sobczak K, Bangel-Ruland N, Leier G, Weber WM. 2010. Endogenous transport systems in the Xenopus laevis oocyte plasma membrane. Methods 51: 183-189.
79. Sonnleitner A, Mannuzzu LD, Terakawa S, Isacoff EY. 2002. Structural rearrangements in single ion channels detected optically in living cells. Proc Natl Acad Sci 99: 12759-12764.
80. Soreq H, Seidman S. 1992. Xenopus oocyte microinjection: From gene to protein. Methods Enzymol 207: 225-265.
81. Steinman RM, Brodie SE, Cohn ZA. 1976. Membrane flow during pinocytosis. A stereologic analysis. J Cell Biol 68: 665-687.
82. Stuhmer W, Parekh AB. 1995. Electrophysiological recordings from Xenopus oocytes. In Single-channel recording (ed. Sakmann B, Neher E), pp. 341-356. Plenum Press, New York.
83. Tompkins-Macdonald GJ, Gallin WJ, Sakarya O, Degnan B, Leys SP, Boland LM. 2009. Expression of a poriferan potassium channel: Insights into the evolution of ion channels in metazoans. J Exp Biol 212: 761-767.
84. Wall DA, Meleka I. 1985. An unusual lysosome compartment involved in vitellogenin endocytosis by Xenopus oocytes. J Cell Biol 101: 1651-1664.
85. Webb AC, Smith LD. 1977. Accumulation of mitochondrial DNA during oogenesis in Xenopus laevis. Dev Biol 56: 219-225.
86. Wolf SF, Schlessinger D. 1977. Nuclear metabolism of ribosomal RNA in growing, methionine-limited, and ethionine-treated HeLa cells. Biochemistry (Mosc) 16: 2783-2791.
87. Yu F, Sun L, Machaca K. 2009. Orai1 internalization and STIM1 clustering inhibition modulate SOCE inactivation during meiosis. Proc Natl Acad Sci 106: 17401-17406.
88. Zampighi GA, Loo DDF, Kreman M, Eskandari S, Wright EM. 1999. Functional and morphological correlates of Connexin50 expressed in Xenopus laevis oocytes. J Gen Physiol 113: 507-523.
89. Zhang D, Boulware MJ, Pendleton MR, Nogi T, Marchant JS. 2007. The inositol (1,4,5) trisphosphate receptor (Itpr) gene family in Xenopus: Identification of type 2 and type 3 IP3 receptor subtypes. Biochem J 404: 383-391.
90. Zhang T, Liu Z, Song W, Du Y, Dong K. 2011. Molecular characterization and functional expression of the DSC1 channel. Insect Biochem Mol Biol 41: 451-458.
91. Zhou W, Chung I, Liu Z, Goldin AL, Dong K. 2004. A voltage-gated calcium-selective channel encoded by a sodium channel-like gene. Neuron 42: 101-112.