Upgrading fungal gene expression on demand: Improved systems for doxycycline-dependent silencing in Aspergillus fumigatus

Applied and Environmental Microbiology

Volumn 79, Issue 5, 2013, Pages 1751-1754

  Helmschrott, Christoph ^a   Sasse, Anna ^b   Samantaray, Sweta ^a   Krappmann, Sven ^b,c   Wagener, Johannes ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

^c UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ASPERGILLUS FUMIGATUS; FUNCTIONAL ELEMENTS; FUNGAL GENES; INTRAMOLECULAR RECOMBINATION; ON DEMANDS;

GENE EXPRESSION;

ASPERGILLUS;

ANTIINFECTIVE AGENT; DOXYCYCLINE;

FUNCTIONAL GROUP; FUNGUS; GENE EXPRESSION; MICROBIAL COMMUNITY; MOLECULAR ANALYSIS;

ARTICLE; ASPERGILLUS FUMIGATUS; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; METHODOLOGY; MICROBIAL GENETICS; MOLECULAR BIOLOGY;

ANTI-BACTERIAL AGENTS; ASPERGILLUS FUMIGATUS; DOXYCYCLINE; GENE EXPRESSION REGULATION, FUNGAL; GENETICS, MICROBIAL; MOLECULAR BIOLOGY;

EID: 84874676006     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.03626-12     Document Type: Article

References (17)

1. Hu W, Sillaots S, Lemieux S, Davison J, Kauffman S, Breton A, Linteau A, Xin C, Bowman J, Becker J, Jiang B, Roemer T. 2007. Essential gene identification and drug target prioritization in Aspergillus fumigatus. PLoS Pathog. 3:e24. doi:10.1371/journal.ppat.0030024.
2. Corbel SY, Rossi FMV. 2002. Latest developments and in vivo use of the Tet system: ex vivo and in vivo delivery of tetracycline-regulated genes. Curr. Opin. Biotechnol. 13:448-452.
3. Sprengel R, Hasan MT. 2007. Tetracycline-controlled genetic switches. Handb. Exp. Pharmacol. 2007:49-72.
4. Bertram R, Hillen W. 2008. The application of Tet repressor in prokaryotic gene regulation and expression. Microb. Biotechnol. 1:2-16.
5. Urlinger S, Baron U, Thellmann M, Hasan MT, Bujard H, Hillen W. 2000. Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity. Proc. Natl. Acad. Sci. U.S.A. 97:7963-7968.
6. Vogt K, Bhabhra R, Rhodes JC, Askew DS. 2005. Doxycycline-regulated gene expression in the opportunistic fungal pathogen Aspergillus fumigatus. BMC Microbiol. 5:1. doi:10.1186/1471-2180-5-1.
7. Meyer V, Wanka F, van Gent J, Arentshorst M, van den Hondel CAMJJ, Ram AFJ. 2011. Fungal gene expression on demand: an inducible, tunable, and metabolism-independent expression system for Aspergillus niger. Appl. Environ. Microbiol. 77:2975-2983.
8. Dichtl K, Helmschrott C, Dirr F, Wagener J. 2012. Deciphering cell wall integrity signalling in Aspergillus fumigatus: identification and functional characterization of cell wall stress sensors and relevant Rho GTPases. Mol. Microbiol. 83:506-519.
9. De Lucas JR, Gregory S, Turner G. 1994. Analysis of the regulation of the Aspergillus nidulans acuD gene, encoding isocitrate lyase, by construction of a hybrid promoter. Mol. Gen. Genet. 243:654-659.
10. Turner G, Brown J, Kerry-Williams S, Bailey AM, Ward M, Punt PJ, van den Hondel CAMJJ. 1989. Analysis of the oliC promoter of Aspegillus nidulans. Found. Biotechnol. Ind. Ferment. Res. 6:101-109.
11. Upshall A, Kumar AA, Bailey MC, Parker MD, Favreau MA, Lewison KP, Joseph ML, Maraganore JM, McKnight GL. 1987. Secretion of active human tissue plasminogen activator from the filamentous fungus Aspergillus nidulans. Biotechnology 5:1301-1304.
12. Krappmann S, Sasse C, Braus GH. 2006. Gene targeting in Aspergillus fumigatus by homologous recombination is facilitated in a nonhomologous end-joining-deficient genetic background. Eukaryot. Cell 5:212-215.
13. Roemer T, Jiang B, Davison J, Ketela T, Veillette K, Breton A, Tandia F, Linteau A, Sillaots S, Marta C, Martel N, Veronneau S, Lemieux S, Kauffman S, Becker J, Storms R, Boone C, Bussey H. 2003. Large-scale essential gene identification in Candida albicans and applications to antifungal drug discovery. Mol. Microbiol. 50:167-181.
14. Henry C, Mouyna I, Latgé J-P. 2007. Testing the efficacy of RNA interference constructs in Aspergillus fumigatus. Curr. Genet. 51:277-284.
15. Yamada O, Ikeda R, Ohkita Y, Hayashi R, Sakamoto K, Akita O. 2007. Gene silencing by RNA interference in the koji mold Aspergillus oryzae. Biosci. Biotechnol. Biochem. 71:138-144.
16. Brakhage AA, Langfelder K. 2002. Menacing mold: the molecular biology of Aspergillus fumigatus. Annu. Rev. Microbiol. 56:433-455.
17. Krappmann S. 2006. Tools to study molecular mechanisms of Aspergillus pathogenicity. Trends Microbiol. 14:356-364.