Clinical scale electroloading of mature dendritic cells with melanoma whole tumor cell lysate is superior to conventional lysate co-incubation in triggering robust in vitro expansion of functional antigen-specific CTL

International Immunopharmacology

Volumn 15, Issue 3, 2013, Pages 488-497

  Wolfraim, Lawrence A ^a   Takahara, Masashi ^b   Viley, Angelia M ^a   Shivakumar, Rama ^a   Nieda, Mie ^b   Maekawa, Ryuji ^b   Liu, Linda N ^a,c   Peshwa, Madhusudan V ^a  

^a MAXCYTE INC   (United States)

^b MEDINET Co Ltd   (Japan)

^c Amplimmune Inc   (United States)

Author keywords

DC vaccine; Dendritic cells; Electroporation; Enhanced potency; Tumor cell lysate

Indexed keywords

CANCER VACCINE; CYCLIC GMP; DENDRITIC CELL VACCINE; GAMMA INTERFERON;

ANTIGEN SPECIFICITY; ARTICLE; CD8+ T LYMPHOCYTE; CELL EXPANSION; CELL LYSATE; CELL STIMULATION; CONTROLLED STUDY; DENDRITIC CELL; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; IN VITRO STUDY; INCUBATION TIME; MATURITY; MELANOMA; NORMAL HUMAN; PRIORITY JOURNAL;

ANTIGEN PRESENTATION; ANTIGENS, CD8; ANTIGENS, NEOPLASM; CANCER VACCINES; CELL DIFFERENTIATION; CELL PROLIFERATION; CELLS, CULTURED; COCULTURE TECHNIQUES; DENDRITIC CELLS; ELECTROPORATION; FEASIBILITY STUDIES; HUMANS; IMMUNOTHERAPY, ADOPTIVE; INTERFERON-GAMMA; LYMPHOCYTE ACTIVATION; MELANOMA; T-LYMPHOCYTES, CYTOTOXIC;

EID: 84874635537     PISSN: 15675769     EISSN: 18781705     Source Type: Journal    
DOI: 10.1016/j.intimp.2013.01.009     Document Type: Article

References (28)

1. M. Nakamura, M. Iwahashi, M. Nakamori, K. Ueda, T. Ojima, and T. Naka Dendritic cells transduced with tumor-associated antigen gene elicit potent therapeutic antitumor immunity: comparison with immunodominant peptide-pulsed DCs Oncology 68 2005 163 170
2. C.J. Kim, D.R. Parkinson, and F. Marincola Immunodominance across HLA polymorphism: implications for cancer immunotherapy J Immunother 21 1998 1 16
3. J.M. Weiss, C. Allen, R. Shivakumar, S. Feller, L.H. Li, and L.N. Liu Efficient responses in a murine renal tumor model by electroloading dendritic cells with whole-tumor lysate J Immunother 28 2005 542 550
4. D. Kim, C. Jeon, J.H. Kim, M.S. Kim, C.H. Yoon, and I.S. Choi Cytoplasmic transduction peptide (CTP): new approach for the delivery of biomolecules into cytoplasm in vitro and in vivo Exp Cell Res 312 2006 1277 1288
5. A. Bonehill, C. Heirman, S. Tuyaerts, A. Michiels, K. Breckpot, and F. Brasseur Messenger RNA-electroporated dendritic cells presenting MAGE-A3 simultaneously in HLA class I and class II molecules J Immunol 172 2004 6649 6657
6. M. Dullaers, K. Breckpot, M.S. Van, A. Bonehill, S. Tuyaerts, and Michiels Side-by-side comparison of lentivirally transduced and mRNA-electroporated dendritic cells: implications for cancer immunotherapy protocols Mol Ther 10 2004 768 779
7. A. Michiels, S. Tuyaerts, A. Bonehill, J. Corthals, K. Breckpot, and C. Heirman Electroporation of immature and mature dendritic cells: implications for dendritic cell-based vaccines Gene Ther 12 2005 772 782
8. V.T. Van, P. Ponsaerts, F. Lardon, G. Nijs, M. Lenjou, and B.C. Van Highly efficient gene delivery by mRNA electroporation in human hematopoietic cells: superiority to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for tumor antigen loading of dendritic cells Blood 98 2001 49 56
9. S. Lohmann, K. Galle, J. Knop, and A.H. Enk CD83 + human dendritic cells transfected with tumor peptide cDNA by electroporation induce specific T-cell responses: a potential tool for gene immunotherapy Cancer Gene Ther 7 2000 605 614
10. + T cell responses in patients with metastatic prostate cancer J Immunol 174 2005 3798 3807
11. I. Strobel, S. Berchtold, A. Gotze, U. Schulze, G. Schuler, and A. Steinkasserer Human dendritic cells transfected with either RNA or DNA encoding influenza matrix protein M1 differ in their ability to stimulate cytotoxic T lymphocytes Gene Ther 7 2000 2028 2035
12. J.A. Kyte, G. Kvalheim, S. Aamdal, S. Saeboe-Larssen, and G. Gaudernack Preclinical full-scale evaluation of dendritic cells transfected with autologous tumor-mRNA for melanoma vaccination Cancer Gene Ther 12 2005 579 591
13. J. Harris, T. Monesmith, A. Ubben, M. Norris, J.H. Freedman, and I. Tcherepanova An improved RNA amplification procedure results in increased yield of autologous RNA transfected dendritic cell-based vaccine Biochim Biophys Acta 1724 2005 127 136
14. Z. Vegh, and A. Mazumder Generation of tumor cell lysate-loaded dendritic cells preprogrammed for IL-12 production and augmented T cell response Cancer Immunol Immunother 52 2003 67 79
15. P. Thumann, I. Moc, J. Humrich, T.G. Berger, E.S. Schultz, and G. Schuler Antigen loading of dendritic cells with whole tumor cell preparations J Immunol Methods 277 2003 1 16
16. J.M. Austyn New insights into the mobilization and phagocytic activity of dendritic cells J Exp Med 183 1996 1287 1292
17. C. Winzler, P. Rovere, M. Rescigno, F. Granucci, G. Penna, and L. Adorini Maturation stages of mouse dendritic cells in growth factor-dependent long-term cultures J Exp Med 185 1997 317 328
18. F. Granucci, E. Ferrero, M. Foti, D. Aggujaro, K. Vettoretto, and P. Ricciardi-Castagnoli Early events in dendritic cell maturation induced by LPS Microbes Infect 1 1999 1079 1084
19. G. Schuler, and R.M. Steinman Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro J Exp Med 161 1985 526 546
20. N. Schaft, J. Dorrie, P. Thumann, V.E. Beck, I. Muller, and E.S. Schultz Generation of an optimized polyvalent monocyte-derived dendritic cell vaccine by transfecting defined RNAs after rather than before maturation J Immunol 174 2005 3087 3097
21. L. Chapatte, M. Ayyoub, S. Morel, A.L. Peitrequin, N. Levy, and C. Servis Processing of tumor-associated antigen by the proteasomes of dendritic cells controls in vivo T-cell responses Cancer Res 66 2006 5461 5468
22. S. Tuyaerts, A. Michiels, J. Corthals, A. Bonehill, C. Heirman, and G.C. De Induction of influenza matrix protein 1 and MelanA-specific T lymphocytes in vitro using mRNA-electroporated dendritic cells Cancer Gene Ther 10 2003 696 706
23. + regulatory T cells by antigen-processing dendritic cells J Exp Med 198 2003 235 247
24. + regulatory T cells including suppressors of alloreactivity Immunol Rev 212 2006 314 329
25. + regulatory T cells from peripheral Foxp3 precursors Blood 110 2007 4293 4302
26. D.J. Chung, M. Rossi, E. Romano, J. Ghith, J. Yuan, and D.H. Munn Indoleamine 2,3-dioxygenase-expressing mature human monocyte-derived dendritic cells expand potent autologous regulatory T cells Blood 114 2009 555 563
27. P. Puccetti, and U. Grohmann IDO and regulatory T cells: a role for reverse signalling and non-canonical NF-kappaB activation Nat Rev Immunol 7 2007 817 823
28. S.W. Tas, M.J. Vervoordeldonk, N. Hajji, J.H. Schuitemaker, K.F. van der Sluijs, and May Noncanonical NF-kappaB signaling in dendritic cells is required for indoleamine 2,3-dioxygenase (IDO) induction and immune regulation Blood 110 2007 1540 1549