Small Hydrophobic Protein of Human Metapneumovirus Does Not Affect Virus Replication and Host Gene Expression In Vitro

PLoS ONE

Volumn 8, Issue 3, 2013, Pages

  de Graaf, Miranda ^a,b   Herfst, Sander ^a   Aarbiou, Jamil ^a   Burgers, Peter C ^a   Zaaraoui Boutahar, Fatiha ^a   Bijl, Maarten ^a   van IJcken, Wilfred ^a   Schrauwen, Eefje J A ^a   Osterhaus, Albert D M E ^a   Luider, Theo M ^a   Scholte, Bob J ^a   Fouchier, Ron A M ^a   Andeweg, Arno C ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

SMALL HYDROPHOBIC PROTEIN; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ARTICLE; CELL CULTURE; CELL LINE; CELL SPREADING; CONTROLLED STUDY; DELETION MUTANT; EPITHELIUM CELL; GENE EXPRESSION; GENETIC ANALYSIS; HUMAN; HUMAN CELL; HUMAN METAPNEUMOVIRUS; HUMAN METAPNEUMOVIRUS INFECTION; HUMAN PRIMARY BRONCHIAL EPITHELIAL CELL; HUMAN TISSUE; IN VITRO STUDY; INTERPHASE; MASS SPECTROMETRY; MICROARRAY ANALYSIS; NONHUMAN; OPEN READING FRAME; PHENOTYPE; PROTEIN EXPRESSION; PROTEIN FUNCTION; VIRUS CELL INTERACTION; VIRUS REPLICATION;

BLOTTING, WESTERN; BRONCHI; CELL LINE; EPITHELIAL CELLS; GENE DELETION; GENE EXPRESSION REGULATION; HUMANS; MASS SPECTROMETRY; METAPNEUMOVIRUS; MICROARRAY ANALYSIS; RETROVIRIDAE PROTEINS, ONCOGENIC; VIRUS REPLICATION;

EID: 84874608475     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0058572     Document Type: Article

References (45)

1. van den Hoogen BG, de Jong JC, Groen J, Kuiken T, de Groot R, et al. (2001) A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med 7: 719-724.
2. van den Hoogen BG, Doornum GJJv, Fockens JC, Cornelissen JJ, Beyer WEP, et al. (2003) Prevalence and clinical symptoms of human metapneumovirus infection in hospitalized patients. J Infect Dis 188.
3. Williams JV, Harris PA, Tollefson SJ, Halburnt-Rush LL, Pingsterhaus JM, et al. (2004) Human metapneumovirus and lower respiratory tract disease in otherwise healthy infants and children. N Engl J Med 350: 443-450.
4. Kahn JS, (2006) Epidemiology of human metapneumovirus. Clin Microbiol Rev 19: 546-557.
5. Schildgen V, van den Hoogen B, Fouchier R, Tripp RA, Alvarez R, et al. (2011) Human Metapneumovirus: lessons learned over the first decade. Clin Microbiol Rev 24: 734-754.
6. van den Hoogen BG, Herfst S, Sprong L, Cane PA, Forleo E, et al. (2004) Antigenic and genetic variability of human metapneumoviruses. Emerg Infect Dis 10: 658-666.
7. Herfst S, de Graaf M, Schickli JH, Tang RS, Kaur J, et al. (2004) Recovery of human metapneumovirus genetic lineages a and B from cloned cDNA. J Virol 78: 8264-8270.
8. Biacchesi S, Skiadopoulos MH, Tran KC, Murphy BR, Collins PL, et al. (2004) Recovery of human metapneumovirus from cDNA: optimization of growth in vitro and expression of additional genes. Virology 321: 247-259.
9. van den Hoogen BG, Bestebroer TM, Osterhaus AD, Fouchier RA, (2002) Analysis of the genomic sequence of a human metapneumovirus. Virology 295: 119-132.
10. Fearns R, Collins PL, (1999) Role of the M2-1 transcription antitermination protein of respiratory syncytial virus in sequential transcription. J Virol 73: 5852-5864.
11. Buchholz UJ, Biacchesi S, Pham QN, Tran KC, Yang L, et al. (2005) Deletion of M2 gene open reading frames 1 and 2 of human metapneumovirus: effects on RNA synthesis, attenuation, and immunogenicity. J Virol 79: 6588-6597.
12. Biacchesi S, Skiadopoulos MH, Yang L, Lamirande EW, Tran KC, et al. (2004) Recombinant human Metapneumovirus lacking the small hydrophobic SH and/or attachment G glycoprotein: deletion of G yields a promising vaccine candidate. J Virol 78: 12877-12887.
13. Teng MN, Whitehead SS, Collins PL, (2001) Contribution of the respiratory syncytial virus G glycoprotein and its secreted and membrane-bound forms to virus replication in vitro and in vivo. Virology 289: 283-296.
14. Bao X, Kolli D, Liu T, Shan Y, Garofalo RP, et al. (2008) Human metapneumovirus small hydrophobic protein inhibits NF-kappaB transcriptional activity. J Virol 82: 8224-8229.
15. Yunus AS, Govindarajan D, Huang Z, Samal SK, (2003) Deduced amino acid sequence of the small hydrophobic protein of US avian pneumovirus has greater identity with that of human metapneumovirus than those of non-US avian pneumoviruses. Virus Res 93: 91-97.
16. Whitehead SS, Bukreyev A, Teng MN, Firestone CY, St Claire M, et al. (1999) Recombinant respiratory syncytial virus bearing a deletion of either the NS2 or SH gene is attenuated in chimpanzees. J Virol 73: 3438-3442.
17. Bukreyev A, Whitehead SS, Murphy BR, Collins PL, (1997) Recombinant respiratory syncytial virus from which the entire SH gene has been deleted grows efficiently in cell culture and exhibits site-specific attenuation in the respiratory tract of the mouse. J Virol 71: 8973-8982.
18. Biacchesi S, Pham QN, Skiadopoulos MH, Murphy BR, Collins PL, et al. (2005) Infection of nonhuman primates with recombinant human metapneumovirus lacking the SH, G, or M2-2 protein categorizes each as a nonessential accessory protein and identifies vaccine candidates. J Virol 79: 12608-12613.
19. Perez M, Garcia-Barreno B, Melero JA, Carrasco L, Guinea R, (1997) Membrane permeability changes induced in Escherichia coli by the SH protein of human respiratory syncytial virus. Virology 235: 342-351.
20. Gan SW, Tan E, Lin X, Yu D, Wang J, et al. (2012) The small hydrophobic protein of the human respiratory syncytial virus forms pentameric ion channels. J Biol Chem.
21. Wilson RL, Fuentes SM, Wang P, Taddeo EC, Klatt A, et al. (2006) Function of small hydrophobic proteins of paramyxovirus. J Virol 80: 1700-1709.
22. Fuentes S, Tran KC, Luthra P, Teng MN, He B, (2007) Function of the respiratory syncytial virus small hydrophobic protein. J Virol 81: 8361-8366.
23. Lin Y, Bright AC, Rothermel TA, He B, (2003) Induction of apoptosis by paramyxovirus simian virus 5 lacking a small hydrophobic gene. J Virol 77: 3371-3383.
24. He B, Lin GY, Durbin JE, Durbin RK, Lamb RA, (2001) The SH integral membrane protein of the paramyxovirus simian virus 5 is required to block apoptosis in MDBK cells. J Virol 75: 4068-4079.
25. de Graaf M, Herfst S, Schrauwen EJ, van den Hoogen BG, Osterhaus AD, et al. (2007) An improved plaque reduction virus neutralization assay for human metapneumovirus. J Virol Methods 143: 169-174.
26. Kuiken T, Van Den Hoogen BG, Van Riel DA, Laman JD, Van Amerongen G, et al. (2004) Experimental Human Metapneumovirus Infection of Cynomolgus Macaques (Macaca fascicularis) Results in Virus Replication in Ciliated Epithelial Cells and Pneumocytes with Associated Lesions throughout the Respiratory Tract. Am J Pathol 164: 1893-1900.
27. van Wetering S, Zuyderduyn S, Ninaber DK, van Sterkenburg MA, Rabe KF, et al. (2007) Epithelial differentiation is a determinant in the production of eotaxin-2 and-3 by bronchial epithelial cells in response to IL-4 and IL-13. Mol Immunol 44: 803-811.
28. Jin H, Zhou H, Cheng X, Tang R, Munoz M, et al. (2000) Recombinant respiratory syncytial viruses with deletions in the NS1, NS2, SH, and M2-2 genes are attenuated in vitro and in vivo. Virology 273: 210-218.
29. Zhang L, Peeples ME, Boucher RC, Collins PL, Pickles RJ, (2002) Respiratory syncytial virus infection of human airway epithelial cells is polarized, specific to ciliated cells, and without obvious cytopathology. J Virol 76: 5654-5666.
30. Bao X, Sinha M, Liu T, Hong C, Luxon BA, et al. (2008) Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis. Virology 374: 114-127.
31. Brasier AR, Jamaluddin M, Casola A, Duan W, Shen Q, et al. (1998) A promoter recruitment mechanism for tumor necrosis factor-alpha-induced interleukin-8 transcription in type II pulmonary epithelial cells. Dependence on nuclear abundance of Rel A, NF-kappaB1, and c-Rel transcription factors. J Biol Chem 273: 3551-3561.
32. Garofalo R, Sabry M, Jamaluddin M, Yu RK, Casola A, et al. (1996) Transcriptional activation of the interleukin-8 gene by respiratory syncytial virus infection in alveolar epithelial cells: nuclear translocation of the RelA transcription factor as a mechanism producing airway mucosal inflammation. J Virol 70: 8773-8781.
33. Skiba M, Mettenleiter TC, Karger A, (2008) Quantitative whole-cell proteome analysis of pseudorabies virus-infected cells. J Virol 82: 9689-9699.
34. Biacchesi S, Murphy BR, Collins PL, Buchholz UJ, (2007) Frequent frameshift and point mutations in the SH gene of human metapneumovirus passaged in vitro. J Virol 81: 6057-6067.
35. Herfst S, de Graaf M, Schrauwen EJ, Sprong L, Hussain K, et al. (2008) Generation of temperature-sensitive human metapneumovirus strains that provide protective immunity in hamsters. J Gen Virol 89: 1553-1562.
36. Buchholz UJ, Finke S, Conzelmann KK, (1999) Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter. J Virol 73: 251-259.
37. Reed LJ, Muench H, (1938) A simple method of estimating fifty percent end points. J Hyg 27: 493-497.
38. Pear WS, Nolan GP, Scott ML, Baltimore D, (1993) Production of high-titer helper-free retroviruses by transient transfection. Proc Natl Acad Sci U S A 90: 8392-8396.
39. Olsen JV, de Godoy LM, Li G, Macek B, Mortensen P, et al. (2005) Parts per million mass accuracy on an Orbitrap mass spectrometer via lock mass injection into a C-trap. Mol Cell Proteomics 4: 2010-2021.
40. Gautier L, Cope L, Bolstad BM, Irizarry RA, (2004) affy--analysis of Affymetrix GeneChip data at the probe level. Bioinformatics 20: 307-315.
41. Smyth GK, (2004) Linear models and empirical bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol 3: Article3.
42. Benjamini Y, Hochberg Y, (1995) Controlling the False Discovery Rate: a Practical and Powerful Approach to Multiple Testing. J R Statist Soc B 51: 289-300.
43. Dekker LJ, Burgers PC, Guzel C, Luider TM, (2007) FTMS and TOF/TOF mass spectrometry in concert: identifying peptides with high reliability using matrix prespotted MALDI target plates. J Chromatogr B Analyt Technol Biomed Life Sci 847: 62-64.
44. Mustafa DA, Burgers PC, Dekker LJ, Charif H, Titulaer MK, et al. (2007) Identification of glioma neovascularization-related proteins by using MALDI-FTMS and nano-LC fractionation to microdissected tumor vessels. Mol Cell Proteomics 6: 1147-1157.
45. Titulaer MK, Siccama I, Dekker LJ, van Rijswijk AL, Heeren RM, et al. (2006) A database application for pre-processing, storage and comparison of mass spectra derived from patients and controls. BMC Bioinformatics 7: 403.