Discrimination of viable from non-viable Gram-negative bacterial pathogens in airborne particles using propidium monoazide-assisted qPCR

Science of the Total Environment

Volumn 449, Issue , 2013, Pages 237-243

  Kaushik, Rajni ^a   Balasubramanian, Rajasekhar ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Airborne particulate matter; Live pathogens; Microbial quality; PMA; QPCR

Indexed keywords

AEROMONAS HYDROPHILA; AIRBORNE PARTICLE; AIRBORNE PARTICULATE MATTERS; BACTERIAL PATHOGENS; BIOMASS-BURNING; COMPLEX MATRICES; CULTURE-BASED METHODS; DNA EXTRACTION; ENVIRONMENTAL SAMPLE; INHIBITION EFFECT; INTERCALATING DYE; KLEBSIELLA PNEUMONIAE; MICROBIAL QUALITY; NON-VIABLE; P-VALUES; PMA; PROPIDIUM; PSEUDOMONAS AERUGINOSA; QPCR; QUANTITATIVE METHOD; REAL-TIME PCR (QPCR); VIABLE BUT NON-CULTURABLE;

BACTERIA; ESCHERICHIA COLI; POLYMERASE CHAIN REACTION;

PATHOGENS;

AZIDE; PROPIDIUM MONOAZIDE; UNCLASSIFIED DRUG;

BACTERIUM; DNA; INHIBITION; OPTIMIZATION; PARTICULATE MATTER; PATHOGEN; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS;

AEROMONAS HYDROPHILA; AIRBORNE PARTICLE; ARTICLE; BACTERIAL VIABILITY; BACTERIUM CULTURE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE DOSAGE; KLEBSIELLA PNEUMONIAE; LIMIT OF DETECTION; NONHUMAN; PARTICULATE MATTER; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PSEUDOMONAS AERUGINOSA; QUANTITATIVE ANALYSIS; RNA EXTRACTION; STATISTICAL SIGNIFICANCE; VALIDATION PROCESS; VIABLE BUT NON CULTURABLE;

AIR MICROBIOLOGY; AZIDES; GRAM-NEGATIVE BACTERIA; POLYMERASE CHAIN REACTION; PROPIDIUM;

EID: 84874396441     PISSN: 00489697     EISSN: 18791026     Source Type: Journal    
DOI: 10.1016/j.scitotenv.2013.01.065     Document Type: Article

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