Methods: Implementation of in vitro and ex vivo phagocytosis and respiratory burst function assessments in safety testing

Journal of Immunotoxicology

Volumn 10, Issue 1, 2013, Pages 106-117

  Freebern, Wendy J ^a   Bigwarfe, Tammy J ^b   Price, Karen D ^a   Haggerty, Helen G ^a  

^a BRISTOL MYERS SQUIBB   (United States)

^b BOEHRINGER INGELHEIM PHARMACEUTICALS INC   (United States)

Author keywords

Immunotoxicology; Innate immune function; Phagocytosis; Respiratory burst

Indexed keywords

4 (4 FLUOROPHENYL) 2 (4 METHYLSULFINYLPHENYL) 5 (4 PYRIDYL)IMIDAZOLE; BMS 582949; MITOGEN ACTIVATED PROTEIN KINASE P38 INHIBITOR; ROTTLERIN; UNCLASSIFIED DRUG; WORTMANNIN;

ARTICLE; CONTROLLED STUDY; EX VIVO STUDY; EXPERIMENTAL DESIGN; FEMALE; FLOW CYTOMETRY; HAPLORHINI; IMMUNOCOMPETENT CELL; IN VITRO STUDY; INNATE IMMUNITY; MALE; MICROTITER PLATE ASSAY; MONOCYTE; NEUTROPHIL; NONHUMAN; OPPORTUNISTIC INFECTION; PHAGOCYTOSIS; PRIORITY JOURNAL; RAT; RESPIRATORY BURST; RESPIRATORY FUNCTION;

ACETOPHENONES; ANDROSTADIENES; ANIMALS; BENZOPYRANS; CELLS, CULTURED; DISEASE MODELS, ANIMAL; DOGS; DRUG EVALUATION, PRECLINICAL; HAPLORHINI; HUMANS; IMIDAZOLES; IMMUNITY, INNATE; IMMUNOLOGIC TESTS; MICE; MONOCYTES; NEUTROPHILS; PHAGOCYTOSIS; PYRIDINES; PYRROLES; RATS; RESPIRATORY BURST; TRIAZINES;

EID: 84874295174     PISSN: 1547691X     EISSN: None     Source Type: Journal    
DOI: 10.3109/1547691X.2012.736427     Document Type: Article

References (29)

1. Burleson, G. R., Dean, J. H., and Munson, A. E. (Eds.) 1995. Methods in Immunotoxicology. Chapter 2. In: Isolation of Alveolar Macrophages, Peritoneal Macrophages, and Kupffer Cells. Vol. 2. (Lewis, J. G., Ed.). New York, NY: Wiley-Liss, Inc., pp. 15-26.
2. Butcher, S. K., Chahal, H., Nayak, L., Sinclair, A., Henriquez, N. V., Sapey, E., O'Mahony, D., and Lord, J. M. 2001. Senescence in innate immune responses: Reduced neutrophil phagocytic capacity and CD16 expression in elderly humans. J. Leukocyte Biol. 70: 881-886.
3. Butcher, S. K., Killampalli, V., Chahal, H., Kaya Alpar, E., and Lord, J. M. 2003. Effect of age on susceptibility to post-traumatic infection in the elderly. Biochem. Soc. Trans. 31: 449-451.
4. Cathcart, R., Schwiers, E., and Ames, B. N. 1983. Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein assay. Anal. Biochem. 134: 111-116.
5. Chan, H., Kedzierska, K., O'Mullane, J., Crowe, S. J., and Jaworowski, A. 2001. Quantifying complement-mediated phagocytosis by human monocyte-derived macrophages. Immunol. Cell Biol. 79: 429-435.
6. Coxon, P. Y, Rane, M. J., Uriarte, S., Powell, D. W., Singh, S., Butt, W., Chen, Q., and McLeish, K. R. 2003. MAPK-activated protein kinase-2 participates in p38 MAPK-dependent and ERK-dependent functions in human neutrophils. Cell Signal. 15: 993-1001.
7. Dzik, J. M., Zielinski, Z., Cielsla, J., and Walajtys-Rode, E. 2010. Trichinella spiralis infection enhances protein kinase C phosphorylation in guinea pig alveolar macrophages. Parasite Immunol. 32: 209-220.
8. Elliot, J. A., and Winn, W. C. 1986. Treatment of alveolar macrophages with cytochalasin D inhibits uptake and subsequent growth of Legionella pneumophila. Infect. Immun. 51: 31-36.
9. Foukas, L. C., Katsoulas, H. L., Paraskevopoulou, N., Metheniti, A., Lambropoulou, M., and Marmaras, V. 1998. Phagocytosis of Escherichia coli by insect hemocytes requires both activation of the Ras/mitogen-activated protein kinase signal transduction pathway for attachment and β3 integrin for internalization. J. Biol. Chem. 273: 14813-14818.
10. Hell, K., Hegener, K., Schnell, R., Mansmann, G., Oberhser, F., Staib, P., Diehl, V., and Engert, A. 1999. Suppressed neutrophil function as a risk factor for severe infection after cytotoxic chemotherapy in patients with acute non-lymphocytic leukemia. J. Ann. Hematol. 78: 73-77.
11. Jackson, J. K., Lauener, R., Duronio, V., and Burt, H. M. 1997. The involvement of phosphatidylinositol 3-kinase in crystal induced human neutrophil activation. J. Rheumatol. 24: 341-348.
12. Klaus, G. G. 1973. Cytochalasin B. Dissociation of pinocytosis and phagocytosis by peritoneal macrophages. Exp. Cell Res. 79: 73-78.
13. Lehmann, D. M., Seneviratne, A. M. P. B., and Smrcka, A. V. 2007. Small molecule disruption of G-protein subunit signaling inhibits neutrophil chemotaxis and inflammation. Mol. Pharmacol. 73: 410-418.
14. Price, K. D. 2010. Bacterial infections in cynomolgus moneys given small molecule immunomodulatory antagonists. J. Immunotoxicol. 7: 128-137.
15. Rosales, C., and Brown, E. J. 1991. Two mechanisms for IgG Fc-receptormediated phagocytosis by human neutrophils. J. Immunol. 146: 3937-3944.
16. Rosenkranz, A. R., Schmaldienst, S., Stuhlmeier, K. M., Chen, W., Knapp, W., and Zlabinger, G. J. 1992. A microplate assay for the detection of oxidative products using 2',7'-dichlorofluorescin diacetate. J. Immunol. Meth. 156: 39-45.
17. Schmaldienst, S., and Horl, W. H. 1996. Bacterial infections during immunosuppression-immunosuppressive agents interfere not only with immune response, but also with polymorphonuclear cell function. Nephrol. Dial. Transplant. 11: 1243.
18. Schnyder, B., Meunier, P. C, and Car, B. D. 1998. Inhibition of kinases impairs neutrophil activation and killing of Staphylococcus aureus. Biochem. J. 331: 489-495.
19. Schopfer, K., and Douglas, S. D. 1976. Neutrophil function in children with kwashiorkor. J. Lab. Clin. Med. 88: 450-461.
20. Takeshige, K., and Minakami, S. 1981. Involvement of calmodulin in phagocytotic respiratory burst of leukocytes. Biochem. Biophys. Res. Com. 99: 484-490.
21. Torres, M., Hall, F. L., and O'Neill, K. 1993. Stimulation of human neutrophils with formyl-methionyl-leucyl-phenylalanine induces tyrosine phosphorylation and activation of two distinct mitogenactivated protein-kinases. J. Immunol. 150: 1563-1577.
22. van der Vliet, A. 2008. NADPH oxidases in lung biology and pathology, host defense enzymes and more. Free Radic. Biol. Med. 44: 938-955.
23. Voloshina, E. V., Prasol, E. A., Grachev, S. V., and Prokhorenko, I. R. 2009. Effect of cytochalasin D on the respiratory burst of primed neutrophils activated with a secondary stimulus. Dokl Biochem. Biophys. 424: 13-15.
24. Wan, C. P., Park, C. S., and Lau, B. H. 1993. A rapid and simple microfluorometric phagocytosis assay. J. Immunol. Meth. 162: 1-7.
25. Wang, R., Town, T., Gokarn, V., Flavell, R. A., and Chandawarkar, R. Y. 2006a. HSP70 enhances macrophage phagocytosis by interaction with lipid raft-associated TLR-7 and up-regulating p38 MAPK and PI3K pathways. J. Surg. Res. 136: 58-69.
26. Wang, X. M., Kim, H. P., Song, R., and Choi, A. M. K. 2006b. Caveolin-1 confers anti-inflammatory effects in murine macrophages via the MKK3/p38 MAPK pathway. Am. J. Resp. Cell Mol. Biol. 34: 434-442.
27. Ward, R. A., Nakamura, M., and McLeish, K. R. 2000. Priming of the neutrophil respiratory burst involves p38 mitogen-activated protein kinase-dependent exocytosis of flavocytochrome b558-containing granules. J. Biol. Chem. 275: 36713-36719.
28. Weinstein, R. J., and Young, L. S. 1976. Neutrophil function in gram-negative rod bacteremia: The interaction between phagocytic cells, infecting organisms, and humoral factors. J. Clin. Invest. 58: 8190-8199.
29. Yona, S., Lin, H., Dri, P., Davies, J. Q., Hayhoe, R. P. G., Lewis, S. M., Heinsbroek, S. E., Brown, K. A., Perretti, M., Hamann, J., Treacher, D. F., Gordon, S., and Stacey, M. 2008. Ligation of the adhesion-GPCR EMR2 regulates human neutrophil function. FASEB J. 22: 741-751.