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Volumn 1, Issue 8, 2012, Pages 954-959

Luminescent iridium(III)-containing block copolymers: Self-assembly into biotin-labeled micelles for biodetection assays

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EID: 84874277632     PISSN: None     EISSN: 21611653     Source Type: Journal    
DOI: 10.1021/mz3001644     Document Type: Article
Times cited : (33)

References (65)
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    • 1(b) Kimyonok, A.; et al. Chem. Mater. 2007, 19, 5602-5608.
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    • Donald, B., McCormick, L. D. W., Eds. Academic Press: New York
    • Bayer, E. A.; Skutelsky, E.; Wilchek, M. In Methods Enzymology; Donald, B., McCormick, L. D. W., Eds.; Academic Press: New York, 1979; Vol. 62, pp 308-315.
    • (1979) Methods Enzymology , vol.62 , pp. 308-315
    • Bayer, E.A.1    Skutelsky, E.2    Wilchek, M.3
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    • To determine the binding affinity of biotin-labelled polymers 7 and 8, we attempted to use the HABA displacement assay. In this commonly used assay, the small molecule HABA (2-(4-hydroxyphenylazo) benzoic acid) is added to streptavidin and shows a characteristic absorbance at 500 nm upon binding. When a biotinylated sample is added to the HABA-streptavidin solution, the strong biotin-streptavidin binding causes the expulsion of the more weakly bound HABA molecules into the aqueous medium, and a decrease in the absorbance at 500 nm is observed. Unfortunately, for these polymer samples the iridium complexes incorporated into the polymers demonstrated a small but noticeable absorbance at 500 nm which interfered with the assay
    • To determine the binding affinity of biotin-labelled polymers 7 and 8, we attempted to use the HABA displacement assay. In this commonly used assay, the small molecule HABA (2-(4-hydroxyphenylazo) benzoic acid) is added to streptavidin and shows a characteristic absorbance at 500 nm upon binding. When a biotinylated sample is added to the HABA-streptavidin solution, the strong biotin-streptavidin binding causes the expulsion of the more weakly bound HABA molecules into the aqueous medium, and a decrease in the absorbance at 500 nm is observed. Unfortunately, for these polymer samples the iridium complexes incorporated into the polymers demonstrated a small but noticeable absorbance at 500 nm which interfered with the assay.


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