Taguchi optimization of duplex PCR for simultaneous identification of Staphylococcus aureus and Clostridium perfringens alpha toxins

FEMS Microbiology Letters

Volumn 340, Issue 2, 2013, Pages 93-100

  Ramakrishna, Uppalapati S ^a   Kingston, Joseph J ^a   Harishchandra Sripathi, Murali ^a   Batra, Harsh V ^a  

^a DEFENCE FOOD RESEARCH LABORATORY   (India)

Author keywords

Alpha toxin; Clostridium perfringens; Staphylococcus aureus; Taguchi method

Indexed keywords

ALPHA TOXIN; BACTERIAL DNA; BACTERIAL TOXIN; GENOMIC DNA;

ANALYTIC METHOD; BACTERIAL GENE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; CLOSTRIDIUM PERFRINGENS; COLONY FORMING UNIT; CONTROLLED STUDY; CPA GENE; CROSS REACTION; DIAGNOSTIC TEST ACCURACY STUDY; DNA PURIFICATION; HLA GENE; LETTER; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; RELIABILITY; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS AUREUS; TAGUCHI METHOD;

BACTERIAL TOXINS; CLOSTRIDIUM INFECTIONS; CLOSTRIDIUM PERFRINGENS; DNA PRIMERS; HUMANS; POLYMERASE CHAIN REACTION; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS;

CLOSTRIDIUM PERFRINGENS; STAPHYLOCOCCUS AUREUS;

EID: 84874213968     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/1574-6968.12070     Document Type: Letter

References (30)

1. Bernheimer AW, Kim KS, Remsen CC, Antanavage J & Watson SW (1972) Factors affecting interaction of staphylococcal α-toxin with membranes. Infect Immun 6: 636-642.
2. Bhakdi S & Tranum-Jensen J (1991) Alpha-toxin of Staphylococcus aureus. Microbiol Rev 55: 733-751.
3. Bowler PG, Duerden BI & Armstrong DG (2001) Wound microbiology and associated approaches to wound management. Clin Microbiol Rev 14: 244-269.
4. Cobb BD & Clarkson JM (1994) A simple procedure for optimising the polymerase chain reaction (PCR) using modified Taguchi methods. Nucleic Acids Res 22: 3801-3805.
5. Dinges MM, Orwin PM & Schlievert PM (2000) Exotoxins of Staphylococcus aureus. Clin Microbiol Rev 13: 16-34.
6. Franklin D & Lowy MD (1998) Staphylococcus aureus infections. N Engl J Med 339: 520-532.
7. Gracias KS & McKillip JL (2004) A review of conventional detection and enumeration methods for pathogenic bacteria in food. Can J Microbiol 50: 883-890.
8. Harrington AT, Mahlen SD & Clarridge JE (2010) Significantly larger numbers of methicillin-resistant Staphylococcus aureus bacteria are recovered from polymicrobial respiratory and wound sites by use of chromogenic primary media than by use of conventional culture. J Clin Microbiol 48: 1350-1353.
9. Heggers JP (1998) Defining infection in chronic wounds: does it matter? J Wound Care 7: 389-392.
10. Hoorfar J, Cook N, Malorny B, Wagner M, Medici DD, Abdulmawjood A & Fach P (2003) Making internal implication control mandatory for diagnostic PCR. J Clin Microbiol 41: 5835.
11. Hoorfar J, Malorny B, Abdulmawjood A, Cook N, Wagner M & Fach P (2004) Internal amplification controls for practical considerations in design of diagnostic PCR assays. J Clin Microbiol 42: 1863-1868.
12. Lavery LA, Harkless LB, Felder-Johnson K & Mundine S (1994) Bacterial pathogens in infected puncture wounds in adults with diabetes. J Foot Ankle Surg 33: 91-97.
13. Le Loir Y, Baron F & Gautier M (2003) Staphylococcus aureus and food poisoning. Genet Mol Res 2: 63-76.
14. MacFarlane MG & Knight BC (1941) The biochemistry of bacterial toxins: the lecithinase activity of Clostridium welchii toxins. Biochem J 35: 884-902.
15. Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3: 208-218.
16. Morgan E (1991) Chemometrics: Experimental Design. John Wiley and Sons, New York.
17. Nagahama M, Okagawa Y, Nakayama T, Nishioka E & Sakurai J (1995) Sitedirected mutagenesis of histidine residues in Clostridium perfringens alpha-toxin. J Bacteriol 177: 1179-1185.
18. Niens M, Spijker GT, Diepstra A & te Meerman GJ (2005) A factorial experiment for optimizing the PCR conditions in routine genotyping. Biotechnol Appl Biochem 42: 157-162.
19. Raahave D, Friis-Moller A, Bjerre-Jespen K, Thiis-Knudsen J & Rasmussen LB (1986) The infective dose of aerobic and anaerobic bacteria in postoperative wound sepsis. Arch Surg 121: 924-929.
20. Ribeiro MG, Lara GHB, Bicudo SD, Souza AVG, Salerno T, Siqueira AK & Geraldo JS (2007) An unusual gangrenous goat mastitis caused by Staphylococcus aureus, Clostridium perfringens and Escherichia coli co-infection. Arq Bras Med Vet Zootec 59: 810-812.
21. Rood JI (1998) Virulence genes of Clostridium perfringens. Annu Rev Microbiol 52: 333-360.
22. Sambrook J, Fritsch EF & Maniatis T (1989) Molecular Cloning: A Laboratory Manual, Vol. 1, 2nd edn, Cold Spring Harbor Laboratory Press, New York.
23. Siebert PD & Larrick JW (1992) Competitive PCR. Nature 359: 557-558.
24. Siqueira FF, Almeida MO, Barroca TM, Horta CC, Carmo AO, Silva RO, Pires PS, Lobato FC & Kalapothakis E (2012) Characterization of polymorphisms and isoforms of the Clostridium perfringens phospholipase C gene (plc) reveals high genetic diversity. Vet Microbiol 159: 397-405.
25. Sun YSmith E, Wolcott R & Dowd SE (2009) Propagation of anaerobic bacteria within an aerobic multi-species chronic wound biofilm model. J Wound Care 18: 426-431.
26. Thanakiatkrai P & Welch L (2012) Using the Taguchi method for rapid quantitative PCR optimization with SYBR Green I. Int J Legal Med 126: 161-165.
27. Titball RW, Naylor CE & Basak AK (1999) The Clostridium perfringens alpha toxin. Anaerobe 5: 51-64.
28. Titball RW, Rood JI & Sussman M (2002) Clostridium perfringens: wound infections. Molecular Med Microbiol 1-3: 1875-1903.
29. Uppalapati SR, Kingston JJ, Murali HS & Batra HV (2012) Generation and characterization of an inter-generic bivalent alpha domain fusion protein αCS from Clostridium perfringens and Staphylococcus aureus for concurrent diagnosis and therapeutic applications. J Appl Microbiol 113: 448-458.
30. Wu VC (2008) A review of microbial injury and recovery methods in food. Food Microbiol 25: 735-744.