Development of high content imaging methods for cell death detection in human pluripotent stem cell-derived cardiomyocytes

Journal of Cardiovascular Translational Research

Volumn 5, Issue 5, 2012, Pages 593-604

  Mioulane, Maxime ^a   Foldes, Gabor ^a   Ali, Nadire N ^a   Schneider, Michael D ^a   Harding, Sian E ^a  

^a NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

Author keywords

Automated assay; Cardiomyocytes apoptosis; High content microscopy; Methods; Necrosis; Stem cell

Indexed keywords

CASPASE 3; CASPASE 7; CHELERYTHRINE; TETRAMETHYLRHODAMINE METHYL ESTER; BENZOPHENANTHRIDINE DERIVATIVE; EFFECTOR CASPASE;

ANALYTIC METHOD; APOPTOSIS; ARTICLE; AUTOMATION; CARDIOTOXICITY; CELL DEATH; CELL DIFFERENTIATION; CELL MEMBRANE PERMEABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVATION; HEART MUSCLE CELL; HEART VENTRICLE REMODELING; HIGH CONTENT MICROSCOPY; HUMAN; HUMAN CELL; IMAGE ANALYSIS; MICROSCOPY; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRION; MOLECULAR IMAGING; PHENOTYPE; PLURIPOTENT STEM CELL; PREDICTIVE VALUE; PRIORITY JOURNAL; SIGNAL PROCESSING; STAINING; ANIMAL; CELL LINE; CELL TRACKING; COMPARATIVE STUDY; DOSE RESPONSE; DRUG EFFECT; FLUORESCENCE MICROSCOPY; HEART MITOCHONDRION; HIGH THROUGHPUT SCREENING; IMAGE PROCESSING; LABORATORY AUTOMATION; METABOLISM; METHODOLOGY; NECROSIS; NEWBORN; NUCLEAR SHAPE; PATHOLOGY; RAT; TIME;

RATTUS;

ANIMALS; ANIMALS, NEWBORN; APOPTOSIS; AUTOMATION, LABORATORY; BENZOPHENANTHRIDINES; CASPASES, EFFECTOR; CELL LINE; CELL MEMBRANE PERMEABILITY; CELL NUCLEUS SHAPE; CELL TRACKING; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME ACTIVATION; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; INDUCED PLURIPOTENT STEM CELLS; MEMBRANE POTENTIAL, MITOCHONDRIAL; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA, HEART; MYOCYTES, CARDIAC; NECROSIS; PHENOTYPE; RATS; TIME FACTORS;

EID: 84874172945     PISSN: 19375387     EISSN: 19375395     Source Type: Journal    
DOI: 10.1007/s12265-012-9396-1     Document Type: Article

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