Exon-Skipping Strategy by Ratio Modulation between Cytoprotective versus Pro-Apoptotic Clusterin Forms Increased Sensitivity of LNCaP to Cell Death

PLoS ONE

Volumn 8, Issue 2, 2013, Pages

  Essabbani, Abdellatif ^a,b   Garcia, Luis ^b,c   Zonetti, Maria Josè ^d   Fisco, Tommaso ^d   Pucci, Sabina ^d   Chiocchia, Gilles ^a,b  

^a INSTITUT COCHIN   (France)

^b LABORATOIRE DES SCIENCES DU CLIMAT ET DE L ENVIRONNEMENT   (France)

^c SORBONNE UNIVERSITÉ   (France)

^d TOR VERGATA UNIVERSITY HOSPITAL   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; CISPLATIN; CLUSTERIN; KU ANTIGEN; LENTIVIRUS VECTOR; PROTEIN BAX; RAD17 PROTEIN; UNCLASSIFIED DRUG;

ALTERNATIVE RNA SPLICING; APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CELL PROLIFERATION; CELL PROTECTION; CELL STRAIN LNCAP; CELLULAR DISTRIBUTION; CLU GENE; CONTROLLED STUDY; DOWN REGULATION; DRUG PROTEIN BINDING; DRUG SENSITIVITY; DRUG TARGETING; EXON SKIPPING; GENE EXPRESSION REGULATION; GENE FUNCTION; HUMAN; HUMAN CELL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; RADIATION EXPOSURE; RADIOSENSITIVITY; SENSITIVITY ANALYSIS; ULTRAVIOLET C RADIATION; VIRAL GENE DELIVERY SYSTEM;

ALTERNATIVE SPLICING; ANTINEOPLASTIC AGENTS; APOPTOSIS; CELL DEATH; CELL LINE, TUMOR; CELL NUCLEUS; CELL PROLIFERATION; CISPLATIN; CLUSTERIN; CYTOPROTECTION; EXONS; HUMANS; MALE; PROSTATIC NEOPLASMS; RADIATION, IONIZING; RNA SPLICING;

EID: 84873932545     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0054920     Document Type: Article

References (26)

1. Jones SE, Jomary C, (2002) Clusterin. Int J Biochem Cell Biol 34: 427-431.
2. Falgarone G, Chiocchia G, (2009) Chapter 8: Clusterin: A multifacet protein at the crossroad of inflammation and autoimmunity. Adv Cancer Res 104: 139-170.
3. Cagnard N, Letourneur F, Essabbani A, Devauchelle V, Mistou S, et al. (2005) Interleukin-32, CCL2, PF4F1 and GFD10 are the only cytokine/chemokine genes differentially expressed by in vitro cultured rheumatoid and osteoarthritis fibroblast-like synoviocytes. Eur Cytokine Netw 16: 289-292.
4. Essabbani A, Margottin-Goguet F, Chiocchia G, (2010) Identification of clusterin domain involved in NF-kappaB pathway regulation. J Biol Chem 285: 4273-4277.
5. Humphreys D, Hochgrebe TT, Easterbrook-Smith SB, Tenniswood MP, Wilson MR, (1997) Effects of clusterin overexpression on TNFalpha- and TGFbeta-mediated death of L929 cells. Biochemistry 36: 15233-15243.
6. Sensibar JA, Sutkowski DM, Raffo A, Buttyan R, Griswold MD, et al. (1995) Prevention of cell death induced by tumor necrosis factor alpha in LNCaP cells by overexpression of sulfated glycoprotein-2 (clusterin). Cancer Res 55: 2431-2437.
7. Miyake H, Hara I, Gleave ME, Eto H, (2004) Protection of androgen-dependent human prostate cancer cells from oxidative stress-induced DNA damage by overexpression of clusterin and its modulation by androgen. Prostate 61: 318-323.
8. French LE, Wohlwend A, Sappino AP, Tschopp J, Schifferli JA, (1994) Human clusterin gene expression is confined to surviving cells during in vitro programmed cell death. J Clin Invest 93: 877-884.
9. Park IS, Che YZ, Bendayan M, Kang SW, Min BH, (1999) Up-regulation of clusterin (sulfated glycoprotein-2) in pancreatic islet cells upon streptozotocin injection to rats. J Endocrinol 162: 57-65.
10. Zhang H, Kim JK, Edwards CA, Xu Z, Taichman R, et al. (2005) Clusterin inhibits apoptosis by interacting with activated Bax. Nat Cell Biol 7: 909-915.
11. Leskov KS, Klokov DY, Li J, Kinsella TJ, Boothman DA, (2003) Synthesis and functional analyses of nuclear clusterin, a cell death protein. J Biol Chem 278: 11590-11600.
12. Markopoulou S, Kontargiris E, Batsi C, Tzavaras T, Trougakos I, et al. (2009) Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin. Febs J 276: 3784-3799.
13. Yang CR, Yeh S, Leskov K, Odegaard E, Hsu HL, et al. (1999) Isolation of Ku70-binding proteins (KUBs). Nucleic Acids Res 27: 2165-2174.
14. Caccamo AE, Scaltriti M, Caporali A, D'Arca D, Scorcioni F, et al. (2003) Nuclear translocation of a clusterin isoform is associated with induction of anoikis in SV40-immortalized human prostate epithelial cells. Ann N Y Acad Sci 1010: 514-519.
15. Caccamo AE, Scaltriti M, Caporali A, D'Arca D, Corti A, et al. (2005) Ca2+ depletion induces nuclear clusterin, a novel effector of apoptosis in immortalized human prostate cells. Cell Death Differ 12: 101-104.
16. Trougakos IP, So A, Jansen B, Gleave ME, Gonos ES, (2004) Silencing expression of the clusterin/apolipoprotein j gene in human cancer cells using small interfering RNA induces spontaneous apoptosis, reduced growth ability, and cell sensitization to genotoxic and oxidative stress. Cancer Res 64: 1834-1842.
17. Trougakos IP, Gonos ES, (2004) Functional analysis of clusterin/apolipoprotein J in cellular death induced by severe genotoxic stress. Ann N Y Acad Sci 1019: 206-210.
18. Gleave M, Miyake H, (2005) Use of antisense oligonucleotides targeting the cytoprotective gene, clusterin, to enhance androgen- and chemo-sensitivity in prostate cancer. World J Urol 23: 38-46.
19. Rizzi F, Bettuzzi S, (2008) Targeting Clusterin in prostate cancer. J Physiol Pharmacol 59Suppl 9: 265-274.
20. Cartegni L, Chew SL, Krainer AR, (2002) Listening to silence and understanding nonsense: exonic mutations that affect splicing. Nat Rev Genet 3: 285-298.
21. Goyenvalle A, Vulin A, Fougerousse F, Leturcq F, Kaplan JC, et al. (2004) Rescue of dystrophic muscle through U7 snRNA-mediated exon skipping. Science 306: 1796-1799.
22. Benchaouir R, Meregalli M, Farini A, D'Antona G, Belicchi M, et al. (2007) Restoration of human dystrophin following transplantation of exon-skipping-engineered DMD patient stem cells into dystrophic mice. Cell Stem Cell 1: 646-657.
23. Lee CH, Jin RJ, Kwak C, Jeong H, Park MS, et al. (2002) Suppression of clusterin expression enhanced cisplatin-induced cytotoxicity on renal cell carcinoma cells. Urology 60: 516-520.
24. Chung J, Kwak C, Jin RJ, Lee CH, Lee KH, et al. (2004) Enhanced chemosensitivity of bladder cancer cells to cisplatin by suppression of clusterin in vitro. Cancer Lett 203: 155-161.
25. Zellweger T, Chi K, Miyake H, Adomat H, Kiyama S, et al. (2002) Enhanced radiation sensitivity in prostate cancer by inhibition of the cell survival protein clusterin. Clin Cancer Res 8: 3276-3284.
26. Gentile M, Latonen L, Laiho M, (2003) Cell cycle arrest and apoptosis provoked by UV radiation-induced DNA damage are transcriptionally highly divergent responses. Nucleic Acids Res 31: 4779-4790.