Measuring methotrexate polyglutamates in red blood cells: A new LC-MS/MS-based method

Analytical and Bioanalytical Chemistry

Volumn 405, Issue 5, 2013, Pages 1673-1681

  Den Boer, E ^a   Meesters, R J W ^b   Van Zelst, B D ^a   Luider, T M ^a   Hazes, J M W ^a   Heil, S G ^a   De Jonge, R ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

^b UNIVERSIDAD DE LOS ANDES   (Colombia)

Author keywords

Erythrocytes; Methotrexate; Methotrexate polyglutamates; Rheumatoid arthritis

Indexed keywords

AMMONIUM BICARBONATE; CELLS; CYTOLOGY; DISEASES; ELECTROSPRAY IONIZATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY;

COEFFICIENT OF VARIATION; ERYTHROCYTES; FOOD AND DRUG ADMINISTRATION; LIQUID CHROMATOGRAPHY ELECTROSPRAY; LOWER LIMIT OF QUANTITATIONS; METHOTREXATE; METHOTREXATE POLYGLUTAMATES; RHEUMATOID ARTHRITIS;

BLOOD;

EID: 84873743490     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-012-6581-7     Document Type: Article

References (26)

1. Strand V, Cohen S, Schiff M, Weaver A, Fleischmann R, Cannon G, Fox R, Moreland L, Olsen N, Furst D, Caldwell J, Kaine J, Sharp J, Hurley F, Loew-Friedrich I (1999) Treatment of active rheumatoid arthritis with leflunomide compared with placebo and methotrexate. Leflunomide Rheumatoid Arthritis Investigators Group. Arch Intern Med 159(21):2542-2550
2. Chabner BA, Allegra CJ, Curt GA, Clendeninn NJ, Baram J, Koizumi S, Drake JC, Jolivet J (1985) Polyglutamation of methotrexate. Is methotrexate a prodrug? J Clin Invest 76(3):907-912. doi: 10.1172/JCI112088
3. Jolivet J, Schilsky RL, Bailey BD, Drake JC, Chabner BA (1982) Synthesis, retention, and biological activity of methotrexate polyglutamates in cultured human breast cancer cells. J Clin Invest 70(2):351-360
4. Dervieux T, Orentas Lein D, Marcelletti J, Pischel K, Smith K, Walsh M, Richerson R (2003) HPLC determination of erythrocyte methotrexate polyglutamates after low-dose methotrexate therapy in patients with rheumatoid arthritis. Clin Chem 49(10):1632-1641
5. van Haandel L, Becker ML, Leeder JS, Williams TD, Stobaugh JF (2009) A novel high-performance liquid chromatography/mass spectrometry method for improved selective and sensitive measurement of methotrexate polyglutamation status in human red blood cells. Rapid Commun Mass Spectrom 23(23):3693-3702. doi: 10.1002/rcm.4300
6. Dervieux T, Furst D, Lein DO, Capps R, Smith K, Caldwell J, Kremer J (2005) Pharmacogenetic and metabolite measurements are associated with clinical status in patients with rheumatoid arthritis treated with methotrexate: results of a multicentred cross sectional observational study. Ann Rheum Dis 64(8):1180-1185. doi: 10.1136/ard.2004.033399
7. Dervieux T, Greenstein N, Kremer J (2006) Pharmacogenomic and metabolic biomarkers in the folate pathway and their association with methotrexate effects during dosage escalation in rheumatoid arthritis. Arthritis Rheum 54(10):3095-3103. doi: 10.1002/art.22129
8. Hroch M, Tukova J, Dolezalova P, Chladek J (2009) An improved high-performance liquid chromatography method for quantification of methotrexate polyglutamates in red blood cells of children with juvenile idiopathic arthritis. Biopharm Drug Dispos 30(3):138-148. doi: 10.1002/bdd.654
9. Becker ML, Gaedigk R, van Haandel L, Thomas B, Lasky A, Hoeltzel M, Dai H, Stobaugh J, Leeder JS (2011) The effect of genotype on methotrexate polyglutamate variability in juvenile idiopathic arthritis and association with drug response. Arthritis Rheum 63(1):276-285. doi: 10.1002/art.30080
10. Becker ML, van Haandel L, Gaedigk R, Lasky A, Hoeltzel M, Stobaugh J, Leeder JS (2010) Analysis of intracellular methotrexate polyglutamates in patients with juvenile idiopathic arthritis: effect of route of administration on variability in intracellular methotrexate polyglutamate concentrations. Arthritis Rheum 62(6):1803-1812. doi: 10.1002/art.27434
11. Cheng HL, Liao YM, Chiou SS, Wu SM (2008) On-line stacking capillary electrophoresis for analysis of methotrexate and its eight metabolites in whole blood. Electrophoresis 29(17):3665-3673. doi: 10.1002/elps.200800029
12. Hayashi H, Fujimaki C, Tsuboi S, Matsuyama T, Daimon T, Itoh K (2008) Application of fluorescence polarization immunoassay for determination of methotrexate-polyglutamates in rheumatoid arthritis patients. Tohoku J Exp Med 215(1):95-101
13. Inoue S, Hashiguchi M, Kawai S, Mochizuki M (2009) Erythrocyte methotrexate-polyglutamate assay using fluorescence polarization immunoassay technique: application to the monitoring of patients with rheumatoid arthritis. Yakugaku Zasshi 129(8):1001-1005
14. Al-Turkmani MR, Law T, Narla A, Kellogg MD (2010) Difficulty measuring methotrexate in a patient with high-dose methotrexate-induced nephrotoxicity. Clin Chem 56(12):1792-1794. doi: 10.1373/clinchem.2010.144824
15. Albertioni F, Rask C, Eksborg S, Poulsen JH, Pettersson B, Beck O, Schroeder H, Peterson C (1996) Evaluation of clinical assays for measuring high-dose methotrexate in plasma. Clin Chem 42(1):39-44
16. van Haandel L, Becker ML, Williams T, Leeder JS, Stobaugh JF (2011) Measurement of methotrexate polyglutamates in human erythrocytes by ion-pair UPLC-MS/MS. Bioanalysis 3(24):2783-2796. doi: 10.4155/bio.11.288
17. Meesters RJ, den Boer E, de Jonge R, Lindemans J, Luider TM (2011) Assessment of intracellular methotrexate and methotrexate-polyglutamate metabolite concentrations in erythrocytes by ultrafast matrix-assisted laser desorption/ionization triple quadrupole tandem mass spectrometry. Rapid Commun Mass Spectrom 25(20):3063-3070. doi: 10.1002/rcm.5202
18. CDER (2001) Center for Drug Evaluation and Research (CDER) Guidance for industry: bioanalytical method validation. CDER, Silver Spring, MD
19. Matuszewski BK, Constanzer ML, Chavez-Eng CM (2003) Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS. Anal Chem 75(13):3019-3030
20. Broughton PM (1984) Carry-over in automatic analysers. J Automat Chem 6(2):94-95. doi: 10.1155/S1463924684000201
21. Vogeser M, Seger C (2010) Pitfalls associated with the use of liquid chromatography-tandem mass spectrometry in the clinical laboratory. Clin Chem 56(8):1234-1244. doi: 10.1373/clinchem.2009.138602
22. Claessen SJ, Hazes JM, Huisman MA, van Zeben D, Luime JJ, Weel AE (2009) Use of risk stratification to target therapies in patients with recent onset arthritis; design of a prospective randomized multicenter controlled trial. BMC Musculoskelet Disord 10:71. doi: 10.1186/1471-2474-10-71
23. de Jong PH, Hazes JM, Barendregt PJ, Huisman M, van Zeben D, van der Lubbe PA, Gerards AH, de Jager MH, de Sonnaville PB, Grillet BA, Luime JJ, Weel AE (2012) Induction therapy with a combination of DMARDs is better than methotrexate monotherapy: first results of the tREACH trial. Ann Rheum Dis. doi: 10.1136/annrheumdis-2011-201162
24. NCCLS (1986) Evaluation of the linearity of quantitative analytical methods, NCCLS document EP6-P. NCCLS 6 (18)
25. Uchiyama M, Matsumoto T, Jimi S, Takamatsu Y, Tamura K, Hara S (2011) Simple and sensitive HPLC method for the fluorometric determination of methotrexate and its major metabolites in human plasma by post-column photochemical reaction. Biomed Chromatogr. doi: 10.1002/bmc.1628
26. Stamp LK, O'Donnell JL, Chapman PT, Zhang M, Frampton C, James J, Barclay ML (2009) Determinants of red blood cell methotrexate polyglutamate concentrations in rheumatoid arthritis patients receiving long-term methotrexate treatment. Arthritis Rheum 60(8):2248-2256. doi: 10.1002/art.24653