Simple and sensitive HPLC method for the fluorometric determination of methotrexate and its major metabolites in human plasma by post-column photochemical reaction

Biomedical Chromatography

Volumn 26, Issue 1, 2012, Pages 76-80

  Uchiyama, Masanobu ^a   Matsumoto, Takanori ^b   Matsumoto, Taichi ^b   Jimi, Shiro ^b   Takamatsu, Yasushi ^a   Tamura, Kazuo ^a   Hara, Shuuji ^b  

^a FUKUOKA UNIVERSITY HOSPITAL   (Japan)

^b FUKUOKA UNIVERSITY   (Japan)

Author keywords

7 hydroxymethotrexate; Fluorometric detection; HPLC; Methotrexate

Indexed keywords

BIOMOLECULES; DEGRADATION; PATIENT TREATMENT; PHOTOCHEMICAL REACTIONS; PLASMA (HUMAN); TRICHLOROACETIC ACID;

7-HYDROXYMETHOTREXATE; FLUOROMETRIC DETECTION; FLUOROMETRIC DETERMINATION; HPLC; HPLC METHOD; HUMAN PLASMAS; METHOTREXATE; OH -; POST-COLUMN; SIMPLE++;

METABOLITES;

2,4 DIAMINO N10 METHYLPTEROIC ACID; 7 HYDROXYMETHOTREXATE; ACETONITRILE; BLOOD EXTRACT; METHOTREXATE; METHOTREXATE DERIVATIVE; TRICHLOROACETIC ACID; UNCLASSIFIED DRUG;

AGED; AQUEOUS SOLUTION; ARTICLE; CASE REPORT; DIAGNOSTIC TEST ACCURACY STUDY; DRUG DETERMINATION; DRUG MEGADOSE; HUMAN; LARGE CELL LYMPHOMA; LIMIT OF QUANTITATION; LIQUID LIQUID EXTRACTION; MALE; PHOTODEGRADATION; PHOTOLYSIS; PLASMA; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SENSITIVITY AND SPECIFICITY; SOLID PHASE EXTRACTION; SPECTROFLUOROMETRY; ULTRAVIOLET IRRADIATION;

EID: 83555163735     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.1628     Document Type: Article

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