Effect of simultaneous down-regulation of pectate lyase and endo-β-1,4-glucanase genes on strawberry fruit softening

Molecular Breeding

Volumn 31, Issue 2, 2013, Pages 313-322

  Youssef, Sabry M ^a,b   Amaya, Iraida ^c   López Aranda, José M ^c   Sesmero, Rafael ^c   Valpuesta, Victoriano ^a   Casadoro, Giorgio ^d   Blanco Portales, Rosario ^e   Pliego Alfaro, Fernando ^a   Quesada, Miguel A ^a   Mercado, José A ^a  

^a UNIVERSITY OF MÁLAGA   (Spain)

^b Ain Shams University   (Egypt)

^c IFAPA CENTRO EL TORUÑO   (Spain)

^d UNIVERSITY OF PADOVA   (Italy)

^e UNIVERSITY OF CÓRDOBA   (Spain)

Author keywords

Cell wall disassembly; Cellulases; Fragaria ananassa; Fruit firmness; Fruit texture; Hemicelluloses; Pectinases; Pectins

Indexed keywords

CELLULOSE; ENCODING (SYMBOLS); GENE EXPRESSION; GENE EXPRESSION REGULATION; PLANTS (BOTANY); SIGNAL ENCODING;

CELL WALL DISASSEMBLY; CELL WALLS; CELLULASE; FRAGARIA ANANASSA; FRUIT FIRMNESS; FRUIT TEXTURE; GLUCANASE; HEMICELLULOSE; PECTIN; PECTINASES;

FRUITS;

FRAGARIA; FRAGARIA X ANANASSA;

EID: 84873738182     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11032-012-9791-y     Document Type: Article

References (33)

1. Barceló M, El Mansouri I, Mercado JA, Quesada MA, Pliego-Alfaro F (1998) Regeneration and transformation via Agrobacterium tumefaciens of the strawberry cultivar Chandler. Plant Cell, Tissue Organ Cult 54: 29-36.
2. Benítez-Burraco A, Blanco-Portales R, Redondo-Nevado J, Bellido ML, Moyano E, Caballero JL, Muñoz-Blanco J (2003) Cloning and characterization of two ripening-related strawberry (Fragaria × ananassa cv. Chandler) pectate lyase genes. J Exp Bot 54: 633-645.
3. Brummell DA, Harpster MH, Civello PM, Palys JM, Bennett AB, Dunsmuir P (1999) Modification of expansin protein abundance in tomato fruit alters softening and cell wall polymer metabolism during ripening. Plant Cell 11: 2203-2216.
4. Brummell DA, Dal Cin V, Crisosto CH, Labavitch JM (2004) Cell wall metabolism during maturation, ripening and senescence of peach fruit. J Exp Bot 55: 2029-2039.
5. Cantu D, Vicente AR, Greve LC, Dewey FM, Bennett AB, Labavitch JM, Powell AL (2008) The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea. Proc Natl Acad Sci USA 105: 859-864.
6. Flors V, Leyva M, Vicedo B, Finiti I, Real MD, García-Agustin P, Bennett AB, González-Bosch C (2007) Absence of the endo-β-1, 4-glucanases Cel1 and Cel2 reduces susceptibility to Botrytis cinerea in tomato. Plant J 52: 1027-1040.
7. Goulao LF, Oliveira CM (2008) Cell wall modifications during fruit ripening: when a fruit is not the fruit. Trends Food Sci Technol 19: 4-25.
8. Huber DJ (1984) Strawberry fruit softening-the potential roles of polyuronides and hemicelluloses. J Food Sci 49: 1310-1315.
9. Jiménez-Bermúdez S, Redondo-Nevado J, Muñoz-Blanco J, Caballero JL, López-Aranda JM, Valpuesta V, Pliego-Alfaro F, Quesada MA, Mercado JA (2002) Manipulation of strawberry fruit softening by antisense expression of a pectate lyase gene. Plant Physiol 128: 751-759.
10. Lee YK, Kim I-J (2011) Modulation of fruit softening by antisense suppression of endo-β-1, 4-glucanase in strawberry. Mol Breed 27: 375-383.
11. Manning K (1991) Isolation of nucleic acids from plants by differential solvent precipitation. Anal Biochem 195: 45-50.
12. Marín-Rodríguez M, Orchard J, Seymour GB (2002) Pectate lyases, cell wall degradation and fruit softening. J Exp Bot 53: 2115-2119.
13. McQueen-Mason SJ, Cosgrove DJ (1994) Disruption of hydrogen bonding between plant cell wall polymers by proteins that induce wall extension. Proc Natl Acad Sci USA 91: 6574-6578.
14. Mercado JA, El Mansouri I, Jiménez-Bermúdez S, Pliego-Alfaro F, Quesada MA (1999) A convenient protocol for extraction and purification of DNA from Fragaria. In Vitro Cell Dev Biol Plant 35: 152-153.
15. Mercado JA, Trainotti L, Jiménez-Bermúdez S, Santiago-Doménech N, Posé S, Donolli R, Barceló M, Casadoro G, Pliego-Alfaro F, Quesada MA (2010) Evaluation of the role of the endo-β-(1, 4)-glucanase gene FaEG3 in strawberry fruit softening. Postharvest Biol Technol 55: 8-14.
16. Mercado JA, Pliego-Alfaro F, Quesada MA (2011) Fruit shelf life and potential for its genetic improvement. In: Jenks MA, Bebeli PJ (eds) Breeding for fruit quality. Wiley-Blackwell, New York, pp 81-104.
17. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497.
18. Murthy BNS, Murch SJ, Saxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Biol Plant 34: 267-275.
19. Nyman M, Wallin A (1992) Transient gene expression in strawberry (Fragaria × ananassa Duch.) protoplasts and the recovery of transgenic plants. Plant Cell Rep 11: 105-108.
20. Palomer X, Llop-Tous I, Vendrell M, Krens FA, Schaart JG, Boone MJ, van der Valk H, Salentijn EMJ (2006) Antisense down-regulation of strawberry endo-β-(1, 4)-glucanase genes does not prevent fruit softening during ripening. Plant Sci 171: 640-646.
21. Perkins-Veazie P (1995) Growth and ripening of strawberry fruit. Hort Rev 17: 267-297.
22. Posé S, García-Gago JA, Santiago-Doménech N, Pliego-Alfaro F, Quesada MA, Mercado JA (2011) Strawberry fruit softening: role of cell wall disassembly and its manipulation in transgenic plants. Genes Genomes Genomics 5: 40-48.
23. Powell ALT, Kalamaki MS, Kurien PA, Gurrieri S, Bennett AB (2003) Simultaneous transgenic suppression of LePG and LeExp1 influences fruit texture and juice viscosity in a fresh market tomato variety. J Agric Food Chem 51: 7450-7455.
24. Quesada MA, Blanco-Portales R, Posé S, García-Gago JA, Jiménez-Bermúdez S, Muñoz-Serrano A, Caballero JL, Pliego-Alfaro F, Mercado JA, Muñoz-Blanco J (2009) Antisense down-regulation of the FaPG1 gene reveals an unexpected central role for polygalacturonase in strawberry fruit softening. Plant Physiol 150: 1022-1032.
25. Santiago-Doménech N, Jiménez-Bemúdez S, Matas AJ, Rose JK, Muñoz-Blanco J, Mercado JA, Quesada MA (2008) Antisense inhibition of a pectate lyase gene supports a role for pectin depolymerization in strawberry fruit softening. J Exp Bot 59: 2769-2779.
26. Spolaore S, Trainotti L, Pavanello A, Casadoro G (2003) Isolation and promoter analysis of two genes encoding different endo-β-1, 4-glucanases in the non-climacteric strawberry. J Exp Bot 54: 271-277.
27. Trainotti L, Spolaore S, Pavanello A, Baldan B, Casadoro G (1999) A novel E-type endo-β-1, 4-glucanase with a putative cellulose-binding domain is highly expressed in ripening strawberry fruits. Plant Mol Biol 40: 323-332.
28. Urbanowicz BR, Bennett AB, del Campillo E, Catalá C, Hayashi T, Henrissat B, Höfte H, McQeen-Mason SJ, Patterson SE, Shoseyov O, Teeri TT, Rose JKC (2007a) Structural organization and a standardized nomenclature for plant endo-1, 4-β-glucanases of glycosyl hydrolase family 9. Plant Physiol 144: 1693-1696.
29. Urbanowicz BR, Catalá C, Irwin D, Wilson DB, Ripoll DR, Rose JKC (2007b) A tomato endo-β -1, 4-glucanase, SlCel9C1, represents a distinct subclass with a new family of carbohydrate binding modules (CBM49). J Biol Chem 282: 12066-12074.
30. Vancanneyt G, Schmidt R, O′Connor-Sanchez A, Willmitzer L, Rocha-Sosa M (1990) Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation. Mol Gen Genet 220: 245-250.
31. Vicente AR, Saladié M, Rose JKC, Labavitch JM (2007) The linkage between cell wall metabolism and fruit softening: looking to the future. J Sci Food Agric 87: 1435-1448.
32. Woolley LC, James DJ, Manning K (2001) Purification and properties of an endo-beta-1, 4-glucanase from strawberry and down-regulation of the corresponding gene, cel1. Planta 214: 11-21.
33. Youssef SM, Jiménez-Bermúdez S, Bellido ML, Martín-Pizarro C, Barceló M, Abdal-Aziz SA, Caballero JL, López-Aranda JM, Pliego-Alfaro F, Muñoz J, Quesada MA, Mercado JA (2009) Fruit yield and quality of strawberry plants transformed with a fruit specific strawberry pectate lyase gene. Sci Hort 119: 120-125.