Evaluation of the role of the endo-β-(1,4)-glucanase gene FaEG3 in strawberry fruit softening

Postharvest Biology and Technology

Volumn 55, Issue 1, 2010, Pages 8-14

  Mercado, José A ^a   Trainotti, Livio ^b   Jiménez Bermúdez, Lara ^c   Santiago Doménech, Nieves ^a   Posé, Sara ^a   Donolli, Rosa ^a   Barceló, Marta ^c   Casadoro, Giorgio ^b   Pliego Alfaro, Fernando ^a   Quesada, Miguel A ^a  

^a UNIVERSITY OF MÁLAGA   (Spain)

^b UNIVERSITY OF PADOVA   (Italy)

^c IFAPA CENTRO EL TORUÑO   (Spain)

Author keywords

Antisense transformation; Cell wall; Cellulases; EGases; Fragaria ananassa; Fruit ripening

Indexed keywords

FRAGARIA; FRAGARIA X ANANASSA;

EID: 70349733392     PISSN: 09255214     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.postharvbio.2009.08.004     Document Type: Article

References (35)

1. Abeles F.B., and Takeda F. Cellulase activity and ethylene in ripening strawberry and apple fruits. Sci. Hortic. 42 (1990) 269-275
2. Barceló M., El-Mansouri I., Mercado J.A., Quesada M.A., and Pliego-Alfaro F. Regeneration and transformation via Agrobacterium tumefaciens of the strawberry cultivar Chandler. Plant Cell Tissue Organ Cult. 54 (1998) 29-36
3. Brummell D.A. Cell wall disassembly in ripening fruit. Funct. Plant Biol. 33 (2006) 103-119
4. Brummell D.A., and Harpster M.H. Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants. Plant Mol. Biol. 47 (2001) 311-340
5. Brummell D.A., Hall B.D., and Bennett A.B. Antisense suppression of tomato endo-1,4-β-glucanase Cel2 mRNA accumulation increases the force required to break fruit abscission zones but does not affect fruit softening. Plant Mol. Biol. 40 (1999) 615-622
6. Dubois M., Gilles K.A., Hamilton J.K., and Smith F. Colorimetric method for determination of sugars and related substances. Anal. Biochem. 28 (1956) 350-356
7. Dumas B., Bottin A., Gaulin E., and Esquerré-Tugayé M.-T. Cellulose-binding domains: cellulose associated-defensive sensing partners?. Trends Plant Sci. 13 (2008) 160-164
8. Durbin M.L., and Lewis L.N. Cellulases in Phaseolus vulgaris. Methods Enzymol. 160 (1988) 342-351
9. Ferrarese L., Trainotti L., Moretto P., Polverino de Laureto P., Rascio N., and Casadoro G. Differential ethylene-inducible expression of cellulase in pepper plants. Plant Mol. Biol. 29 (1995) 735-747
10. Flors V., Leyva M.O., Vicedo B., Finiti I., Real M.D., García-Agustín P., Bennett A.B., and González-Bosch C. Absence of the endo-β-1,4-glucanases Cel1 and Cel2 reduces susceptibility to Botrytis cinerea in tomato. Plant J. 52 (2007) 1027-1040
11. Gross K.C. A rapid and sensitive spectrophotometric method for assaying polygalacturonase using 2-cyanoacetamide. HortScience 17 (1982) 933-934
12. Harpster M.H., Brummell D.A., and Dunsmuir P. Expression analysis of a ripening-specific, auxin-repressed endo-1,4-β-glucanase gene in strawberry. Plant Physiol. 118 (1998) 1307-1316
13. Harpster M.H., Brummell D.A., and Dunsmuir P. Suppression of a ripening-related endo-1,4-β-glucanase in transgenic pepper fruit does not prevent depolymerization of cell wall polysaccharides during ripening. Plant Mol. Biol. 50 (2002) 345-355
14. Harpster M.H., Dawson D.M., Nevins D.J., Dunsmuir P., and Brummell D.A. Constitutive overexpression of a ripening-related pepper endo-1,4-β-glucanase in transgenic tomato fruit does not increase xyloglucan depolymerization or fruit softening. Plant Mol. Biol. 50 (2002) 357-369
15. Henrissat B., Coutinho P.M., and Davies G.J. A census of carbohydrate-active enzymes in the genome of Arabidopsis thaliana. Plant Mol. Biol. 47 (2001) 55-72
16. Höfgen R., and Willmitzer L. Biochemical and genetic analysis of different patatin isoforms expressed in various organs of potato (Solanum tuberosum). Plant Sci. 66 (1990) 221-230
17. Huber D.J. Strawberry fruit softening-the potential roles of polyuronides and hemicelluloses. J. Food Sci. 49 (1984) 1310-1315
18. Jiménez-Bermúdez S., Redondo-Nevado J., Muñoz-Blanco J., Caballero J.L., López-Aranda J.M., Valpuesta V., Pliego-Alfaro F., Quesada M.A., and Mercado J.A. Manipulation of strawberry fruit softening by antisense expression of a pectate lyase gene. Plant Physiol. 128 (2002) 751-759
19. 13C NMR characterization of cell walls of ripening strawberries. Can. J. Bot. 75 (1997) 1957-1964
20. Llop-Tous I., Domínguez-Puigjaner E., Palomer X., and Vendrell M. Characterization of two divergent endo-β-1,4-glucanase cDNA clones highly expressed in the nonclimateric strawberry fruit. Plant Physiol. 119 (1999) 1415-1421
21. Medina-Escobar N., Cardenas J., Moyano E., Caballero J.L., and Muñoz-Blanco J. Cloning, molecular characterization and expression pattern of a strawberry ripening-specific cDNA with sequence homology to pectate lyase from higher plants. Plant Mol. Biol. 34 (1997) 867-877
22. Nehra N.S., Kartha K.K., Stushnoff C., and Giles K.L. The influence of plant growth regulator concentrations and callus age on somaclonal variation in callus culture regenerants of strawberry. Plant Cell Tissue Organ Cult. 29 (1992) 257-268
23. O'Donoghue E.M., and Huber D.J. Modification of matrix polysaccharides during avocado (Persea americana) fruit ripening: an assessment of the role of Cx-cellulase. Physiol. Plant. 86 (1992) 33-42
24. Palomer X., Llop-Tous I., Vendrell M., Krens F.A., Schaart J.G., Boone M.J., van der Valk E., and Salentijn E.M.J. Antisense down-regulation of strawberry endo-β-(1,4)-glucanase genes does not prevent fruit softening during ripening. Plant Sci. 171 (2006) 640-646
25. Quesada M.A., Blanco-Portales R., Posé S., García-Gago J.A., Jiménez-Bermúdez S., Muñoz-Serrano A., Caballero J.L., Pliego-Alfaro F., Mercado J.A., and Muñoz-Blanco J. Antisense down-regulation of the FaPG1 gene reveals an unexpected central role for polygalacturonase in strawberry fruit softening. Plant Physiol. 150 (2009) 1022-1032
26. Rosli H.G., Civello P.M., and Martinez G.A. Changes in cell wall composition of three Fragaria × ananassa cultivars with different softening rate during ripening. Plant Physiol. Biochem. 42 (2004) 823-831
27. Santiago-Doménech N., Jiménez-Bermúdez S., Matas A.J., Rose J.K.C., Muñoz-Blanco J., Mercado J.A., and Quesada M.A. Antisense inhibition of a pectate lyase gene supports a role for pectin depolymerization in strawberry fruit softening. J. Exp. Bot. 59 (2008) 2769-2779
28. Schaart J.G., Salentijn E.M.J., and Krens F.A. Tissue-specific expression of a β-glucuronidase reporter gene in transgenic strawberry (Fragaria × ananassa) plants. Plant Cell Rep. 21 (2002) 313-319
29. Spolaore S., Trainotti L., Pavanello A., and Casadoro G. Isolation and promoter analysis of two genes encoding different endo-β-1,4-glucanases in the non-climacteric strawberry. J. Exp. Bot. 54 (2003) 271-277
30. Trainotti L., Ferrarese L., Dalla Vecchia F., Rascio N., and Casadoro G. Two divergent endo-β-1,4-glucanases contribute to the softening of the strawberry fruits. J. Plant Physiol. 154 (1999) 355-362
31. Trainotti L., Spolaore S., Pavanello A., Baldan B., and Casadoro G. A novel E-type endo-β-1,4-glucanase with a putative cellulose-binding domain is highly expressed in ripening strawberry fruits. Plant Mol. Biol. 40 (1999) 323-332
32. Urbanowicz B.R., Bennett A.B., del Campillo E., Catalá C., Hayashi T., Henrissat B., Höfte H., McQeen-Mason S.J., Patterson S.E., Shoseyov O., Teeri T.T., and Rose J.K.C. Structural organization and a standardized nomenclature for plant endo-1,4-β-glucanases of glycosyl hydrolase family 9. Plant Physiol. 144 (2007) 1693-1696
33. Urbanowicz B.R., Catalá C., Irwin D., Wilson D.B., Ripoll D.R., and Rose J.K.C. A tomato endo-β-1,4-glucanase, SlCel9C1, represents a distinct subclass with a new family of carbohydrate binding modules (CBM49). J. Biol. Chem. 282 (2007) 12066-12074
34. Woolley L.C., James D.J., and Manning K. Purification and properties of an endo-β-1,4-glucanase from strawberry and down-regulation of the corresponding gene, cel1. Planta 214 (2001) 11-21
35. Youssef S.M., Jiménez-Bermúdez S., Luz Bellido M., Martín-Pizarro C., Barceló M., Abdal-Aziz S.A., Caballero J.L., López-Aranda J.M., Pliego-Alfaro F., Muñoz J., Quesada M.A., and Mercado J.A. Fruit yield and quality of strawberry plants transformed with a fruit specific strawberry pectate lyase gene. Sci. Hortic. 119 (2009) 120-125