Modulation of fruit softening by antisense suppression of endo-β-1,4-glucanase in strawberry

Molecular Breeding

Volumn 27, Issue 3, 2011, Pages 375-383

  Lee, Young Koung ^a   Kim, In Jung ^b  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Jeju National University   (South Korea)

Author keywords

Ascorbate peroxidase; Endo 1,4 glucanase; Firmness; Fragaria ananassa; Transgenic strawberry

Indexed keywords

PLANTS (BOTANY); POLYMERASE CHAIN REACTION; TISSUE CULTURE;

ANTISENSE; ASCORBATE PEROXIDASE; CAMV 35S PROMOTER; ENDO-Β-1,4-GLUCANASE; FIRMNESS; FRAGARIA ANANASSA; GLUCANASE; TRANSGENIC LINES; TRANSGENIC STRAWBERRY; TRANSGENICS;

FRUITS;

FRAGARIA; FRAGARIA X ANANASSA;

EID: 79952106101     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11032-010-9438-9     Document Type: Article

References (28)

1. Brummell DA, Hall BD, Bennett AB (1999) Antisense suppression of tomato endo-1, 4-beta-glucanase Cel2 mRNA accumulation increases the force required to break fruit abscission zones but does not affect fruit softening. Plant Mol Biol 40: 615-622.
2. Cass LG, Kirven KA, Christoffersen RE (1990) Isolation and characterization of a cellulase gene family member expressed during avocado fruit ripening. Mol Gen Genet 223: 76-86.
3. Christoffersen RE, Tucker ML, Laties GG (1984) Cellulase gene expression in ripening avocado fruit: the accumulation of cellulase mRNA and protein as demonstrated by cDNA hybridization and immunodetection. Plant Mol Biol 3: 385-391.
4. Davies C, Boss PK, Robinson SP (1997) Treatment of grape berries, a nonclimacteric fruit with a synthetic auxin, retards ripening and alters the expression of developmentally regulated genes. Plant Physiol 115: 1155-1161.
5. del Campillo E, Bennett AB (1996) Pedicel breakstrength and cellulase gene expression during tomato flower abscission. Plant Physiol 111: 813-820.
6. Ferrarese L, Trainotti L, Moretto P, Polverino de Laureto P, Rascio N, Casadoro G (1995) Differential ethylene-inducible expression of cellulase in pepper plants. Plant Mol Biol 29(4): 735-747.
7. Harpster MH, Brummell DA, Dunsmuir P (1998) Expression analysis of a ripening-specific, auxin-repressed endo-1, 4-beta-glucanase gene in strawberry. Plant Physiol 118: 1307-1316.
8. Holsters M, de Waele D, Depicker A, Messens E, van Montagu M, Schell J (1978) Transfection and transformation of Agrobacterium tumefaciens. Mol Gen Genet 163: 181-187.
9. Huber DJ (1984) Strawberry fruit softening: the potential roles of polyuronides and hemicelluloses. J Food Sci 49: 1310-1315.
10. Kim IJ, Chung WI (1998a) Molecular characterization of a cytosolic ascorbate peroxidase in strawberry fruit. Plant Sci 133: 69-77.
11. Kim IJ, Chung WI (1998b) Isolation of genomic DNA containing a cytosolic ascorbate peroxidase gene (ApxSC) from strawberry (Fragaria × ananassa). Biosci Biotechnol Biochem 62: 1358-1363.
12. Lashbrook CC, Gonzalez-Bosch C, Bennett AB (1994) Two divergent endo-beta-1, 4-glucanase genes exhibit overlapping expression in ripening fruit and abscising flowers. Plant Cell 6: 1485-1493.
13. Llop-Tous I, Domínguez-Puigjaner E, Palomer X, Vendrell M (1999) Characterization of two divergent endo-beta-1, 4-glucanase cDNA clones highly expressed in the nonclimacteric strawberry fruit. Plant Physiol 119: 1415-1422.
14. Nehra NS, Chibbar RN, Kartha KK, Datla RSS, Crosby WL, Stushnoff C (1990) Genetic transformation of strawberry by Agrobacterium tumefaciens using a leaf disk regeneration system. Plant Cell Rep 9: 293-298.
15. Nogata Y, Ohta H, Voragen AGJ (1993) Polygalacturonase in strawberry fruit. Phytochemistry 34: 617-620.
16. Park JI, Lee YK, Chung WI, Lee IH, Choi JH, Lee WM, Ezura H, Lee SP, Kim IJ (2006) Modification of sugar composition in strawberry fruit by antisense suppression of an ADP-glucose pyrophosphorylase. Mol Breed 17: 269-279.
17. Pear JR, Sanders RA, Summerfelt KR, Martineau B, Hiatt WR (1993) Simultaneous inhibition of two tomato fruit cell wall hydrolases, pectinmethylesterase and polygalacturonase, with antisense gene constructs. Antisense Res Dev 3: 181-190.
18. Quesada MA, Blanco-Portales R, Posé S, García-Gago JA, Jiménez-Bermúdez S, Muñoz-Serrano A, Caballero JL, Pliego-Alfaro F, Mercado JA, Muñoz-Blanco J (2009) Antisense down-regulation of the FaPG1 gene reveals an unexpected central role for polygalacturonase in strawberry fruit softening. Plant Physiol 150: 1022-1032.
19. Santiago-Doménech N, Jiménez-Bemúdez S, Matas AJ, Rose JKC, Muñoz-Blanco J, Mercado JA, Quesada MA (2008) Antisense inhibition of a pectate lyase gene supports a role for pectin depolymerization in strawberry fruit softening. J Exp Bot 59: 2769-2779.
20. Shani Z, Dekel M, Tsabary G, Shoseyov O (1997) Cloning and chracterization of elongation specific endo-β-1, 4-beta-glucanase (cell1) from Arabidopsis thaliana. Plant Mol Biol 34: 837-842.
21. Sheehy RE, Kramer M, Hiatt WR (1988) Reduction of polygalacturonase activity in tomato fruit by antisense RNA. Proc Natl Acad Sci USA 85: 8805-8809.
22. Smith CJ, Watson CF, Morris PC, Bird CR, Seymour GB, Gray JE, Arnold C, Tucker GA, Schuch W, Harding S (1990) Inheritance and effect on ripening of antisense polygalacturonase genes in transgenic tomatoes. Plant Mol Biol 14: 369-379.
23. Spolaore S, Trainotti L, Pavanelo A, Casadoro G (2003) Isolation and promoter analysis of two genes encoding different endo-beta-1, 4-glucanases in the non-climacteric strawberry. J Exp Bot 54: 271-277.
24. Trainotti L, Spolaore S, Ferrarese L, Casadoro G (1997) Characterization of ppEG1, a member of a multigene family which encodes endo-beta-1, 4-glucanase in peach. Plant Mol Biol 34: 791-802.
25. Trainotti L, Ferrarese L, Vecchia FD, Rascio N, Casadoro G (1999a) Two different Endo-β-1, 4 glucanase contribute to the softening of the strawberry fruit. J Plant Physiol 154: 355-362.
26. Trainotti L, Spolaore S, Pavanello A, Baldan B, Casadoro G (1999b) A novel E-type endo-beta-1, 4-glucanase with a putative cellulose-binding domain is highly expressed in ripening strawberry fruits. Plant Mol Biol 40: 323-332.
27. Tucker ML, Milligan SB (1991) Sequence analysis and comparison of avocado fruit and bean abscission cellulases. Plant Physiol 95: 928-933.
28. Zuo J, Niu QW, Nishizawa N, Wu Y, Kost B, Chua NH (2000) KORRIGAN, an Arabidopsis endo-1, 4-beta-glucanase, localizes to the cell plate by polarized targeting and is essential for cytokinesis. Plant Cell 12: 1137-1152.