A high-throughput-compatible fluorescence anisotropy-based assay for competitive inhibitors of Escherichia coli UDP-N-acetylglucosamine acyltransferase (LpxA)

Journal of Biomolecular Screening

Volumn 18, Issue 3, 2013, Pages 341-347

  Shapiro, Adam B ^a   Ross, Philip L ^a   Gao, Ning ^a   Livchak, Stephania ^a   Kern, Gunther ^a   Yang, Wei ^a   Andrews, Beth ^a   Thresher, Jason ^a  

^a ASTRAZENECA   (United Kingdom)

Author keywords

anti infective drugs; enzyme assays or enzyme kinetics; fluorescence methods; high throughput screening

Indexed keywords

3 HYDROXYMYRISTIC ACID; ACYL CARRIER PROTEIN; ACYLTRANSFERASE; ACYLTRANSFERASE INHIBITOR; FLUORESCENT DYE; LPXA ENZYME; MYRISTIC ACID DERIVATIVE; PEPTIDE 920; PEPTIDE DERIVATIVE; UDP 3 O ( 3 HYDROXYMYRISTOYL) N ACETYLGLUCOSAMINE; UDP N ACETYLGLUCOSAMINE ACYLTRANSFERASE INHIBITOR; UNCLASSIFIED DRUG; URIDINE DERIVATIVE; URIDINE DIPHOSPHATE N ACETYLGALACTOSAMINE;

ANISOTROPY; ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; CRYSTAL STRUCTURE; DRUG DETERMINATION; ESCHERICHIA COLI; FLUORESCENCE POLARIZATION IMMUNOASSAY; HIGH THROUGHPUT SCREENING; IC 50; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN PURIFICATION;

ACYL CARRIER PROTEIN; ACYLTRANSFERASES; BINDING, COMPETITIVE; CATALYTIC DOMAIN; DRUG EVALUATION, PRECLINICAL; ENZYME INHIBITORS; ESCHERICHIA COLI; FLUORESCENCE POLARIZATION; HIGH-THROUGHPUT SCREENING ASSAYS; INHIBITORY CONCENTRATION 50; LIGANDS; LIPID A; MYRISTIC ACIDS; PEPTIDES; URIDINE DIPHOSPHATE N-ACETYLGLUCOSAMINE;

EID: 84873651173     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057112462062     Document Type: Article

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